Agnieszka Czarnocka-Cieciura,Natalia Gumińska
Agnieszka Czarnocka-Cieciura
In this study, we introduce a novel approach for analysing long, repetitive genomic sequences. Our methods significantly advance research on rDNA polymorphism. First, we describe a technique for isolating high-molecular-weight DNA from indi...
Twenty-Five Years of the Environmental Stress Response and the Enduring Power of Yeast in Stress Biology [0.03%]
二十年的环境压力响应及酵母在压力生物学中的持久力
Audrey P Gasch
Audrey P Gasch
All organisms must be able to sense and respond to adverse environments, especially those that threaten cellular integrity. The age of genomics clarified the breadth and specificity of cellular stress responses, including in free-living mic...
Yeast at Forty [0.03%]
酿酒酵母的四十年研究历程
Stephen G Oliver
Stephen G Oliver
Edward Archer,Roy S K Walker,Paige E Erpf et al.
Edward Archer et al.
The evolution of the yeast, Saccharomyces cerevisiae, from a genetically tractable model organism to a chassis for genome-scale engineering represents one of the most influential trajectories in eukaryotic biology. The Synthetic Yeast Genom...
The Role of Mannose in Saccharomyces pastorianus Flocculation During Industrial Brewing Fermentations [0.03%]
葡萄糖基酵母 flocculation 的作用机制研究
Devanshu V Mehta,Mary T Serviss,Keith R Schneider et al.
Devanshu V Mehta et al.
Yeast flocculation is a phenomenon in which yeast zymolectins bind to mannose on adjacent cells resulting in aggregation within fermentation media. Flocculation has been extensively studied and is often assumed to occur only after media sug...
EL222-Based Optogenetic Gene Regulation in Methylotrophic Yeasts: Mechanisms, Applications, and Future Directions [0.03%]
基于EL222的甲基营养型酵母光遗传学基因调控：机制、应用及未来展望
Riya Joon,Rhythm Phutela,Prashant Khare
Riya Joon
Methylotrophic yeasts such as Pichia pastoris are widely used for heterologous protein production because they contain strong and tightly regulated promoters. However, the use of methanol as an inducer presents several practical challenges,...
A New Set of Optogenetic Switches in Yeast Based on the BcWCL1 Photoreceptor [0.03%]
基于BcWCL1光受体的酵母新型.optogenetic开关体系
Matías Guerrero,Josefa Oyarzún,Diego Ruiz et al.
Matías Guerrero et al.
Optogenetic switches are molecular systems enabling light-controlled gene expression. These systems are based on the reconstitution of chimeric Transcription Factors (TFs) including a DNA-binding Domain (DBD), a photoreceptor domain, and an...
Schizosaccharomyces Orthogroup (SOG) Resource: A Web Platform for Exploring Gene Conservation in Fission Yeasts [0.03%]
裂殖酵母直系同源群(SOG)资源：探索裂殖酵母中基因保守性的网络平台
Guo-Song Jia,Fang Suo,Ambre Noly et al.
Guo-Song Jia et al.
The fission yeast Schizosaccharomyces pombe is a prominent model organism widely used to investigate fundamental cellular mechanisms. In addition to S. pombe, the genus Schizosaccharomyces includes six other species-S. octosporus, S. japoni...
Glucose Restriction Extends Chronological Lifespan of Budding Yeast by Avoiding Commonly Used Auxotrophic Marker Nutrient Starvation [0.03%]
通过避免常用的 auxotrophic 标记营养匮乏来延长芽殖酵母的生物学寿命
Yukinori Ozaki,Taku Ozaki,Qiu-Mei Zhang-Akiyama
Yukinori Ozaki
Dietary restriction (DR) is a robust lifespan-extending intervention across species. While budding yeast is a fundamental model for DR, results from glucose restriction (GR) often show inconsistencies. This may stem from auxotrophic markers...
CRISPR/Cas9 Genome Engineering in Non-Conventional Oleaginous Yeasts: Applications, Challenges, and Prospects [0.03%]
基于CRISPR/Cas9基因编辑的非常见产油酵母遗传操作：应用、挑战与前景
Rodrigo Gonçalves Dias,Fernanda Pinheiro Moreira Freitas,Eduardo Luís Menezes de Almeida et al.
Rodrigo Gonçalves Dias et al.
Given the biotechnological potential of yeast-derived oils for oleochemical production, genes encoding lipid metabolism enzymes are key targets for metabolic engineering. Genetic engineering tools such as Clustered Regularly Interspaced Sho...