The Impact of Different Alkylation Quenching Methods on Tryptic Activity and Protein Identification in Proteomics Sample Preparation [0.03%]
用于蛋白质组学样本制备的甲基化淬灭方法对胰蛋白酶活性和蛋白质鉴定的影响
Yuan Gao,Min Wang,Lulu Wang et al.
Yuan Gao et al.
The reduction and alkylation steps are crucial in shotgun proteomics sample preparation to ensure efficient protein digestion and prevent the reformation of artefactual disulfide bonds following proteolysis. Excessive alkylation reagents ca...
Development of a Rapid LC-MS/MS Method and Its Application for the Pharmacokinetic Analysis of Pacritinib in Rats [0.03%]
帕克利替尼在大鼠体内快速液相色谱-质谱分析方法的建立及其药代动力学研究
Ya Meng Wu,Kai Zheng,Luo Yi Huang et al.
Ya Meng Wu et al.
Pacritinib is a novel medication with certain limitations and unknowns in therapeutic applications. Due to the increased frequency of side effects such as fungal infections, nausea, and vomiting, the potential for drug interactions is signi...
Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method Development and Validation to Quantify Simultaneously Six Urinary DIALKYL Phosphate Metabolites of Organophosphorus Pesticides [0.03%]
超高效液相色谱-串联质谱法定量同时测定六种有机磷农药尿样中二烷基磷酸酯代谢产物的方法学研究及验证
Ngo Kien Đuc,Truong Nhat Khanh,Phan Nguyen Truong Thang et al.
Ngo Kien Đuc et al.
The exposure to chemical pesticides is one of the world's concerns, especially in Vietnam, which is becoming a key player in global agriculture. The chronic long-term exposure to pesticides, especially organophosphorus groups poses increase...
Rapid Identification of Chemical Compounds in Danzhi Jiangtang Capsule Using Ultra-Performance Liquid Chromatography Quadrupole Time-of-Flight Mass Spectrometry Combined With Multiple Data Processing Techniques [0.03%]
基于UPLC-Q-TOF-MS和多种数据处理技术的丹栀降糖胶囊快速鉴定方法
Xiaojie Fu,Junting Zhou,Jindong Zhao et al.
Xiaojie Fu et al.
Danzhi Jiangtang capsule (DJC) is a traditional Chinese medicine prescription that has been clinically used to treat Type 2 diabetes mellitus and its complications. However, research on the chemical compounds present in DJC remains limited....
Spatially Resolved Metabolomics Reveals Metabolic Heterogeneity Among Pulmonary Fibrosis [0.03%]
空间分辨代谢组学揭示肺纤维化中的代谢异质性
Shengxi Li,Cong Li,Wei Sun et al.
Shengxi Li et al.
Pulmonary fibrosis (PF) is a chronic and progressive lung disease with fatal consequences. The study of PF is challenging due to the complex mechanism involved, the need to understand the heterogeneity and spatial organization within lung t...
Identification of Botanicals Based on Their Mass Spectrum Fingerprints Using Ultra-Performance Liquid Chromatography-Mass Spectrometry [0.03%]
基于超高效液相色谱质谱指纹图的中药识别研究
Akhilesh Kumar,Dipak Kumar Mishra,Sanjeev Kanojiya
Akhilesh Kumar
In the current scenario, herbal raw materials are identified via morphotaxonomy, microscopic pharmacognosy, or DNA barcoding. However, these methods do not reveal their chemical integrity, while plant raw materials play a crucial role in th...
Sulfuric/Sulfurous Acids Induce Self-Protection of Phospholipids Against Air-Water Interfacial Ozonolysis [0.03%]
硫酸和亚硫酸对磷脂在空气-水界面上抗臭氧化的诱导自保护作用
Hong Zhang,Yuqing Niu,Jiaqi Xing et al.
Hong Zhang et al.
Sulfides are ubiquity in atmosphere and can convert to be H2SO3/H2SO4, which could affect the inflammatory responses induced by ozone. However, the mixing effect and mechanism of H2SO3/H2SO4 and ozone at molecular-level on the lung surface ...
Multi-Omic Evaluation of PLK1 Inhibitor-Onvansertib-In Colorectal Cancer Spheroids [0.03%]
PLK1抑制剂Onvansertib在结直肠癌类器官中的多组学评估
Brian D Fries,Emily R Sekera,Joseph H Holbrook et al.
Brian D Fries et al.
Polo-like kinase 1 (Plk1) is a serine/threonine kinase involved in regulating the cell cycle. It is activated by aurora kinase B along with the cofactors Borealin, INCE, and survivin. Plk1 is involved in the development of resistances to ch...
Automated and Fully Validated High-Throughput LC-MS/MS Assay for Analyzing Multiple Drugs of Abuse in Oral Fluids Using Novel Features in Sample Preparation and Chromatographic Conditions [0.03%]
一种用于分析口腔液体中多种滥用药物的自动化和完全验证的高通量LC-MS/MS测定方法，采用样品处理和色谱条件的新功能
Joakim Tan,Alexia Rylski,Anders Bergqvist et al.
Joakim Tan et al.
Oral fluid sampling offers advantages over other biological matrices, mainly due to its noninvasive procedure avoiding privacy intrusion. The fully automated sample preparation procedure is based on salting-out assisted liquid-liquid extrac...
Comparison of Dip-it-DART-Orbitrap-MS With Nitrogen Plasma to HPLC/Orbitrap-MS in Profiling Aromatic Glycoconjugation in White Grapes [0.03%]
比较Dip-it DART Orbitrap MS与氮等离子体HPLC Orbitrap MS在白葡萄芳香糖蛋白特征分析中的应用
Mariusz Dziadas,Henryk Jeleń
Mariusz Dziadas
Direct analysis of aromatic glycosidic precursors in plants has posed an analytical challenge for decades. Traditional techniques, such as SPE-GC/MS, primarily provided information on volatile aglycones released through hydrolysis. However,...
Comparative Study
Journal of mass spectrometry : JMS. 2025 May;60(5):e5130. DOI:10.1002/jms.5130 2025