Proteome constrained metabolic modeling of Sus scrofa muscle stem cells for cultured meat production [0.03%]
基于猪肌肉干细胞蛋白质组的培养肉代谢模型构建与优化
Sizhe Qiu,Eliska Kratochvilova,Wei E Huang et al.
Sizhe Qiu et al.
Cultured meat has recently emerged as a sustainable alternative to traditional livestock farming and gained attention as a promising future protein source. Herein, the Sus scrofa muscle stem cell is a commonly used cell source in the cell p...
Biosynthetic platform for orsellinic acid-derived meroterpenoids in Escherichia coli [0.03%]
基于金丝桃酸的茋-倍半萜杂合生物碱的合成代谢工程平台菌株构建
Itsuki Tomita,Takahiro Bamba,Takanobu Yoshida et al.
Itsuki Tomita et al.
Orsellinic acid (OSA)-derived meroterpenoids, that have an OSA backbone, are plant-derived natural products that have attracted considerable attention as pharmaceutical precursors because of their diverse pharmacological activities. Therefo...
An automated platform for accelerating and focusing adaptive laboratory evolution [0.03%]
一种加速和聚焦适应性实验室进化的自动化平台
Peter Ruppen,Maximilian Ole Bahls,Michael Sebastian Gerlt et al.
Peter Ruppen et al.
The rate of change in adaptive laboratory evolution (ALE), in which a population of microorganisms is continuously cultivated under a specific selective pressure, is controlled by the cellular mutagenesis rate and the randomness of where in...
Systematic metabolic engineering of an industrial Penicillium citrinum for one-step pravastatin production [0.03%]
系统地对工业黄青霉进行代谢工程改造以一步法生产普伐他汀
Mengyi Xiong,Zhiqiang Du,Zehao Fan et al.
Mengyi Xiong et al.
Pravastatin is a widely prescribed cholesterol-lowering drug known for its superior water solubility and favorable pharmacokinetics. However, its industrial production remains constrained by an inefficient two-step fermentation process, par...
Engineering Escherichia coli for the production of saturated archaeal lipids [0.03%]
工程改造大肠杆菌用于合成饱和古菌脂质
Jiayi Jiang,Mirthe Hoekzema,Ruben Andringa et al.
Jiayi Jiang et al.
Archaeal membrane phospholipids have a different chemical composition than the phospholipids found in bacteria and eukaryotes. Typically, in archaea, phospholipids consist of saturated isoprenoid chains that are ether-bonded to glycerol 1-p...
Machine learning-driven optimization of metabolic balance for β-carotene production [0.03%]
基于机器学习的代谢平衡优化以增强β-胡萝卜素生产效能
Weiming Tu,Jiabao Xu,Yongshuo Ma et al.
Weiming Tu et al.
Balancing metabolic pathways is critical for engineering microbial platforms to efficiently and robustly synthesize value-added bioproducts. In the oleaginous yeast Yarrowia lipolytica engineered for β-carotene production, lipid synthesis ...
PET-FBA: A lightweight enzyme allocation and thermodynamics-constrained flux analysis approach to explore Escherichia coli metabolic adaptation to intracellular acidification [0.03%]
一种轻量级的酶分配和热力学约束通量分析方法用于探索大肠杆菌对细胞内酸化的代谢适应性
Chao Wu,Jeffrey N Law,Onyeka Onyenemezu et al.
Chao Wu et al.
Escherichia coli employs diverse strategies to adapt to acidic environments that disrupt enzyme activity and the thermodynamic feasibility of essential reactions. To understand the impact of pH stress on cell metabolism, we present the PET-...
Lacto-N-tetraose biosynthesis from lactose via metabolically rewired Escherichia coli [0.03%]
通过代谢重组的大肠杆菌从乳糖合成乳糖四糖(Lnt)
Dileep Sai Kumar Palur,Shannon R Pressley,Alex McGill et al.
Dileep Sai Kumar Palur et al.
Human milk oligosaccharides (HMOs), such as lacto-N-tetraose (LNT), play critical roles in infant health by shaping gut microbiota and modulating immune function. While LNT is already produced at industrial scales, efficient microbial route...
Metabolic flux and resource balance in the oleaginous yeast Rhodotorula toruloides [0.03%]
红球固氮酵母(Rhodotorula toruloides)的代谢流和资源平衡状况分析
Eric J Mooney,Patrick F Suthers,Wheaton L Schroeder et al.
Eric J Mooney et al.
The yeast Rhodotorula toruloides is a promising bioproduction organism due to its high lipid yields and ability to grow on cheap and abundant substrates. Quantitative, systems-level assessment of its metabolic activity is accordingly merite...
Pilot production of P(3HB-co-4HB) by engineered Halomonas bluephagenesis harboring an endogenous plasmid grown on glucose [0.03%]
葡萄糖培养含内源质粒的工程假单胞醋酸钙菌蓝噬菌体株生产P(3HB-co-4HB)小试研究
Rou Wen,Yiling Chen,Jiale Wang et al.
Rou Wen et al.
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB) or P34HB) is a promising biopolyester for applications in food packaging, medical sutures, drug delivery, and tissue engineering due to its tunable thermomechanical properties. How...