Establishing biosynthetic pathways for the production of nicotinic acid and trigonelline in Escherichia coli [0.03%]
在大肠杆菌中建立生物合成途径以生产烟酸和三磷酸腺苷葡萄糖 pyrophosphatase enzymedeficiency
Tong Wang,Liangyu Lu,Xiaolei Wang et al.
Tong Wang et al.
Microbial cell factories offer a sustainable platform for the production of natural products, but their development is often constrained by inefficient or poorly characterized native biosynthetic pathways. Nicotinic acid (NA) and its N-meth...
Metabolic engineering of Corynebacterium glutamicum for the production of the low-caloric natural sweetener D-allulose via phosphorylated intermediates [0.03%]
通过磷酸化中间体代谢工程化谷氨酸棒杆菌生产非糖式甜味剂D-阿洛糖
Alexander Lehnert,Maja Deditius,Astrid Wirtz et al.
Alexander Lehnert et al.
D-Allulose is a natural, low-calorie sweetener providing ∼70% of the sweetness of D-sucrose but ≤10% of its caloric value, making it an attractive alternative to conventional sugars. Recently, a phosphorylation-dephosphorylation pathway f...
Exploring shikimate pathway enzyme diversity for engineering of glycopeptide antibiotic biosynthesis [0.03%]
探索希波马酸途径酶的多样性以用于糖肽抗生素生物合成工程研究
Jens-Peter Rodler,Oleksandr Yushchuk,Athina Gavriilidou et al.
Jens-Peter Rodler et al.
Supply of pathway precursors often limits the biosynthesis of specialized metabolites. Type I-IV glycopeptide antibiotics (GPAs) contain non-proteinogenic aromatic amino acids, requiring a sustained flux through the shikimate pathway. To se...
Adaptive evolution of engineered Saccharomyces cerevisiae in favored and unusual chemical environments [0.03%]
模式酵母工程菌在理想及非传统环境中的适应性进化研究
Natalia Kakko von Koch,An Nguyen,Olli Lohilahti et al.
Natalia Kakko von Koch et al.
Using engineered microbial cells for chemical production from renewable resources could replace oil-based chemistry. However, it is underexplored how the chemical production by engineered microbial cells is affected by them being exposed to...
Engineering the robustness of Meyerozyma guilliermondii for efficient 2-phenylethanol biosynthesis [0.03%]
高效合成苯乙醇的毕赤酵母耐久性工程菌株的构建与优化
Feng Guo,Wenqi Ma,Zijian Wan et al.
Feng Guo et al.
Microbial cell factories have enabled the sustainable synthesis of numerous chemicals, yet the production of cytotoxic compounds remains restricted by limited understanding of toxicity mechanisms and intrinsic tolerance. Here, by integratin...
Amino acid deaminase outperforms transaminase in α-keto acid production from amino acids [0.03%]
氨基酸脱氨基酶在α-酮酸从头合成中优于转氨酶
Ryo Nasuno,Hisashi Kudo,Keiji Fushimi et al.
Ryo Nasuno et al.
α-Keto acids are central metabolic intermediates that serve as precursors for a broad range of valuable compounds, including alcohols, organic acids, and alkanes. Therefore, efficient biosynthesis of α-keto acids from α-amino acids is es...
A database of over 15,000 strain design publications reveals a conserved set of metabolic engineering targets across microbial hosts and products [0.03%]
一个超过15000篇菌株设计文献的数据库揭示了微生物宿主和产物中一致的代谢工程目标体系
Elisa Márquez-Zavala,Francesca Di Bartolomeo,Daniel Machado
Elisa Márquez-Zavala
Microbial biotechnology has the potential to address several societal issues through the sustainable production of industrially relevant compounds. Despite decades of successful cases, rational engineering of microbial metabolism is still a...
Pseudomonas vancouverensis as a platform for one-carbon (C1) assimilation and polyhydroxyalkanoate accumulation [0.03%]
青岛希瓦氏菌是一碳(C1)同化和聚羟基烷酸积累的平台
Emil C Ørsted,Meg Walsh,Kevin O&#x;Connor et al.
Emil C Ørsted et al.
Metabolic engineering calls for new bacterial chassis that combine robust metabolic and physiological traits with genetic tractability. We developed Pseudomonas vancouverensis DhA-51, a native one-carbon (C1)-trophic strain, into a platform...
Rational engineering to enhance C8-fatty acid biosynthesis in Picosynechococcus sp. PCC 7002 [0.03%]
理性改造提高原绿球菌C8脂肪酸生物合成能力
Baltazar E Zuniga,Joshua P Abraham,Juthamas Jaroensuk et al.
Baltazar E Zuniga et al.
Medium-chain fatty acids (e.g., C8-C14) are important biofuel precursors that can be produced from CO2 by cyanobacteria and other photoautotrophs. However, cyanobacteria naturally direct a relatively small fraction of fixed carbon to lipid ...
Systematic study of genomic loci in Escherichia coli B and K12 for genomic integration: application in plasmid-free astaxanthin production [0.03%]
系统研究大肠杆菌B和K12基因组位点的基因组整合：无质粒虾青素生产中的应用
Ngoc-Phuong-Thao Nguyen,Leonard Ong,Congqiang Zhang
Ngoc-Phuong-Thao Nguyen
Escherichia coli is a major workhorse for industrial biotechnology, yet reliance on plasmids is hindered by intrinsic instability. While genomic integration offers superior stability, it remains challenging for integrating large DNA fragmen...