Synergistic Co-utilization of the Prenol and Methylerythritol Phosphate Pathways for Maximizing cis-Abienol Biosynthesis in Escherichia coli [0.03%]
在大肠杆菌中协同利用戊糖和甲基赤藓糖醇磷酸途径最大化顺式阿倍烯醇生物合成
Xinyi Zhang,Sudha Shukal,Xiaohui Lim et al.
Xinyi Zhang et al.
Cis-abienol is a high-value diterpenoid and a key precursor in the fragrance industry. However, its limited natural availability has shifted focus toward efficient microbial production. In this study, we developed a systematic metabolic eng...
Metabolic rewiring and biosensor-assisted screening for high-level putrescine production from xylose in Escherichia coli [0.03%]
基于肠道细菌的尸胺代谢重构和生物传感筛选生产菌株
Haolin Han,Runjia Xie,Guangqi Shan et al.
Haolin Han et al.
Lignocellulosic biomass represents an abundant and renewable feedstock for sustainable biomanufacturing, with its major constituent xylose offering a cost-effective carbon source for producing value-added chemicals. Putrescine is an importa...
Expanding the scope of redox-balance growth coupling techniques with a carbon co-feeding strategy [0.03%]
基于碳辅助呼吸的技术扩大了氧化还原平衡生长耦合的范围
Aidan E Cowan,Bridgie Cawthon,Mason Hillers et al.
Aidan E Cowan et al.
Metabolic engineering to produce molecules not naturally synthesized by the host often requires directed evolution to improve pathway enzyme performance. Growth-coupled selection can dramatically increase directed-evolution throughput, and ...
The state of metabolic engineering in the versatile bacterial cell factory Cupriavidus necator H16 [0.03%]
柄杆菌属底盘细胞工厂的代谢工程化改造及其应用研究进展——聚焦希瓦氏菌H16
Neha Bansal,Tytti Jämsä,Suzan Yilmaz et al.
Neha Bansal et al.
Cupriavidus necator strain H16 has emerged as a versatile microbial chassis for sustainable bioproduction due to its metabolic flexibility, enabling growth on a wide range of substrates, including H2 + CO2, formate, and organic carbon (wast...
Systems metabolic engineering overcomes bottlenecks for high-level L-cysteine production [0.03%]
系统代谢工程克服高效L-半胱氨酸生产的瓶颈问题
Mingli Zhao,Teng Chen,Zhaolin Huang et al.
Mingli Zhao et al.
The sustainable biomanufacturing of L-cysteine, a sulfur-containing amino acid with extensive industrial applications, is hindered by interconnected metabolic bottlenecks including stringent regulation mechanisms, product cytotoxicity, and ...
Engineering the intracellular lifetime of pathway enzymes to enhance microbial myo-inositol production [0.03%]
工程化合成途径酶的胞内半衰期以增强微生物肌醇产量
Shanshan Zhang,Yu Zhang,Muhammad Hassan Butt et al.
Shanshan Zhang et al.
The continuous turnover of pathway enzymes imposes a significant metabolic burden on engineered cells and often limits production efficiency. However, enzyme lifetime is rarely considered a fundamental parameter in pathway design and optimi...
De novo biosynthesis of halogenated benzoxazinoids in Nicotiana benthamiana [0.03%]
烟草中从头合成卤代苯并恶唑烷酮化合物
Susan Schlüter,Matilde Florean,Yoko Nakamura et al.
Susan Schlüter et al.
Halogenation of natural products can improve their bioactivity, pharmacokinetics, and commercial value, yet sustainable production of halogenated small molecules remains challenging. Benzoxazinoids (BXDs) are indole-derived plant specialize...
Multifaceted metabolic engineering of Streptomyces hygroscopicus for milbemycins overproduction [0.03%]
多重代谢工程改造束状芽孢杆菌高产米尔倍美林类抗生素
Jiafeng Xu,Guosong Zheng,Bin Ni et al.
Jiafeng Xu et al.
Milbemycins, 16-membered macrolide antibiotics structurally similar to avermectins, have significant applications in agricultural and veterinary pest control. However, their industrial production is hindered by low fermentation yields. To a...
Enzyme engineering for optimizing biosynthesis of 2,4-dihydroxybutyric acid via the synthetic threose-dependent glycolaldehyde assimilation (STEGA) pathway [0.03%]
通过合成赤藓糖依赖型甘醛同化(STEGA)途径优化2,4-二羟基丁酸生物合成的酶工程
Linxuan Wen,Alrik Titze,Christopher M Topham et al.
Linxuan Wen et al.
Ethylene glycol is a potential feedstock for next-generation biorefineries, as it can be derived from both plastic waste and carbon dioxide. The synthetic D-threose-dependent glycolaldehyde assimilation (STEGA) pathway, which is orthogonal ...
Protein phase separation for enhanced production of 3-hydroxypropionate and polyhydroxybutyrate by Halomonas [0.03%]
盐单胞菌中蛋白质相分离增强3-羟基丙酸和聚羟基丁酸生产的能力研究
Xueqing Yi,Ge Zhang,Zhongnan Zhang et al.
Xueqing Yi et al.
Microbial cell factories for sustainable bioproduction often suffer from trade-offs between cell growth and product synthesis. Spatiotemporal regulation of metabolic pathways offers a potential solution to this challenge. In this study, we ...