Consumption of the degradation products of nylon 6 and nylon 6,6 as the sole carbon and nitrogen source by engineered Acinetobacter baylyi [0.03%]
工程菌芽胞杆菌属巴尔氏酵母（Acinetobacter baylyi）以尼龙6和尼龙6，6降解产物作为唯一碳源和氮源的消耗研究
Mirna Kheir Gouda,Christopher A Voigt
Mirna Kheir Gouda
Nylons (polyamides) are the second most-produced synthetic fiber globally, but they are not biodegradable and even their monomeric constituents are difficult for microbes to consume. The breakdown of nylon 6,6 and nylon 6 leads to a mixture...
Boosting L-homoserine production by non-auxotrophic E. coli strain via iterative rational design and tolerance engineering [0.03%]
通过迭代的理性设计和耐受性工程提高非营养缺陷型大肠杆菌菌株L-羟丝氨酸产量
Jie Zheng,Bo Yu
Jie Zheng
L-Homoserine is a non-proteinogenic amino acid utilized as a precursor in the biosynthesis of various value-added chemicals. In this study, we developed a new L-homoserine-producing strain by integrating rational re-engineering with toleran...
Development, optimization, and application of an episomal plasmid system for Rhodotorula toruloides [0.03%]
用于Rhodotorula toruloides的表型质粒系统的开发、优化及应用研究
Longyuan Shi,Hao Xu,Huimin Zhao
Longyuan Shi
Rhodotorula toruloides is an emerging oleaginous yeast with strong potential as a microbial cell factory for the production of acetyl-CoA-derived bioproducts. However, engineering of this organism has been limited by the absence of a functi...
Genome-based optimization of psilocybin and N,N-dimethyltryptamine biosynthetic pathways in E. coli using CRISPR-associated transposases [0.03%]
基于基因组的优化利用CRISPR相关转座酶在大肠杆菌中优化裸盖菇素和N,N-二甲基脱水角旋苔碱生物合成途径
Zachary N Abrahms,Mohammad Majdi,Siena M Madsen et al.
Zachary N Abrahms et al.
Stable, high-level biosynthesis of complex natural products requires precise control of heterologous pathway expression, yet transcriptional architectures optimized on plasmids often fail when transferred to the chromosome. Here, we present...
Development of a Noncanonical Redox Cofactor Platform in Saccharomyces cerevisiae [0.03%]
在酿酒酵母中开发非经典的红ox辅因子平台
William B Black,Kristina McKee,Kelly N Richardson et al.
William B Black et al.
Saccharomyces cerevisiae is a keystone host for biomanufacturing, yet its metabolic engineering is often complicated by the crosstalk between heterologous pathways and native metabolism. In particular, allocating and balancing the universal...
MAPS: a marker-free genome integration system for Komagataella phaffii enabling co-production of natural pigments and industrial enzymes [0.03%]
一种无标记基因组整合系统MAPS用于实现天然色素和工业酶的协同生产
Sarah Schmid,Charis Wardell,Anna Maria Hatzl et al.
Sarah Schmid et al.
Marker-free genome engineering remains an important objective in the metabolic engineering of Komagataella phaffii, particularly for the construction of stable and regulatory-compliant industrial production strains. Here, we present MAPS, a...
Multi-lab, multi-enzyme study demonstrates the versatility of bacterial microcompartment shells as a modular platform for confined biocatalysis [0.03%]
多实验室、多种酶研究表明细菌微室壳的多功能性使其可作为限定空间生物催化的模块化平台
Sreeahila Retnadhas,Nicholas M Tefft,Yali Wang et al.
Sreeahila Retnadhas et al.
Bacterial microcompartments (BMCs) are proteinaceous organelles that spatially organize metabolic reactions in bacteria and represent an attractive scaffold for pathway engineering. Here, we present a proof-of-concept in vitro study demonst...
Deep learning unlocks sequence-divergent synthetic promoters to empower Streptomyces natural product engineering [0.03%]
深度学习解锁序列发散的合成启动子以增强链霉菌天然产物工程化
Qun Zhou,Ye Wang,Jixin Dong et al.
Qun Zhou et al.
Streptomyces are renowned for their unparalleled capacity to produce bioactive natural products, making them prime candidates for industrial antibiotic manufacturing. However, Streptomyces lags behind E. coli and yeast in genetic tool devel...
Production of redox-active organic electrolyte candidates using the oleaginous yeast Yarrowia lipolytica as a heterologous cell factory [0.03%]
以类酵母假丝酵母为异源细胞工厂生产具有氧化还原活性的有机电解质候选物
Mihaela Bejenari,Katharina Røhrt Bech,Klaus Ringsborg Westphal et al.
Mihaela Bejenari et al.
Fungal quinone polyketides are promising bio-electrolyte candidates for aqueous organic redox flow batteries (AORFBs) but are difficult to produce efficiently and at scale in native filamentous fungi. Here, we engineered Yarrowia lipolytica...
Engineering complex phenotypes in Halomonas bluephagenesis TD01 via large-fragment manipulation and multiplex base editing [0.03%]
通过大片段操作和多重碱基编辑在Halomonas bluephagenesis TD01中工程化复杂的表型
Yu-Hang Zhang,Yuan Yuan,Bai-Tao Chen et al.
Yu-Hang Zhang et al.
Halomonas bluephagenesis is a representative platform strain of next generation industrial biotechnology (NGIB), enabling contamination-resistant open fermentation due to inherent tolerance to high salinity and alkalinity. However, progress...