13C metabolic flux analysis and gene expression analysis of perfusion culture reveal metabolic bottlenecks for stable glycosylation profiles of biologics [0.03%]
基于灌流培养的代谢通量分析与基因表达分析揭示生物药稳定糖基化模式的代谢瓶颈
Sarah A Sacco,Tiffany Tang,Sean Engels et al.
Sarah A Sacco et al.
Perfusion Chinese hamster ovary (CHO) cell culture offers potential benefits over fed-batch cultures, including higher productivity and a reduced production footprint. However, maintaining a stable profile of product quality attributes (PQA...
De novo biosynthesis of the polymethoxylated flavones eupatilin and jaceosidin in Saccharomyces cerevisiae [0.03%]
从头合成新补加茋和菊塞定在酿酒酵母中的聚合甲氧基黄酮类化合物
Jingyan Liu,Tianzhen Sun,Zhongjun Liu et al.
Jingyan Liu et al.
Eupatilin (EUP) and jaceosidin (JAC) are bioactive polymethoxylated flavones (PMFs) derived from Artemisia argyi. However, their sustainable production is hindered by the low abundance of this plant and the challenges of regioselective chem...
Metabolic rewiring overcomes physiological constraints in Sphingobium lignivorans SYK-6 for valorization of industrial lignin streams [0.03%]
代谢重构克服了Sphingobium lignivorans SYK-6生理限制以实现工业木质素的高值化转换
Akito Mizuide,Ryo Kato,Masaya Fujita et al.
Akito Mizuide et al.
Lignin-rich industrial streams represent an abundant but underutilized source of renewable aromatic carbon. Efficient biological conversion requires microbial hosts capable of metabolizing chemically diverse lignin-derived aromatic compound...
An Nguyen,Merja Penttilä,Alexander D Frey et al.
An Nguyen et al.
Biopolymer chitin has properties such as biocompatibility, strength, chemical functionality, and biodegradability that are attractive for applications. It could be biotechnologically produced using fungi, but the economical feasibility depe...
Programmable and controllable sexual life cycle for improved evolution in Komegataella phaffii [0.03%]
可编程和可控有性生活周期在毕赤酵母Komegataellaphaffii进化中的应用
Lili Song,Yanyan Wu,Li Hua et al.
Lili Song et al.
The sexual life cycle represents a natural engine for promoting genetic diversity in yeast. However, harnessing it for controllable, rapid evolution in industrial yeast strains remains a big challenge. Here, we developed quantitative mating...
Evolution-guided high yield production of potent Gαq/11-signalling inhibitors FR900359 and YM-254890 [0.03%]
进化导向的高效生产强力Gαq/11信号抑制剂FR900359和YM-254890
Yiwen Zhou,Xinyi Zhao,Xingyu Zhao et al.
Yiwen Zhou et al.
Heterotrimeric G proteins modulate a wide range of important intracellar effectors and play indispensable roles in diverse human physiological processes. The cyclic depsipeptides FR900359 and YM-254890 have emerged as potent and selective i...
Engineering a microbial platform for the biosynthesis of anthranilic acid and its derivatives [0.03%]
工程微平台生物合成芥喃酸及其衍生物
Wen Zhang,Xiaxia Zhang,Liangyu Lu et al.
Wen Zhang et al.
Anthranilic acid (ANTA) and its derivatives, such as N-methylanthranilic acid (NMAA) and 3-hydroxyanthranilic acid (3-HAA), are valuable aromatic chemicals with wide applications in the pharmaceutical and chemical industries due to their an...
Metabolic engineering strategies for producing decanoic acid and related oleochemicals: 1-decanol, 2-nonanone, and poly(3-hydroxydecanoate) in Escherichia coli [0.03%]
用于生产癸酸和相关脂肪化工产品的代谢工程策略：在大肠杆菌中合成月桂醇、壬酮和聚癸二酰乙二醇酯
Shivangi Mishra,Yun Su,Shufang Huang et al.
Shivangi Mishra et al.
Medium chain oleochemicals are an important class of renewable chemicals with broad industrial applications; however, their sustainable microbial production remains challenging. In this study we developed a versatile metabolic engineering a...
Reconstitution of Human Milk Oligosaccharide Biosynthesis in Cultured Mammalian Cells [0.03%]
在培养的哺乳动物细胞中重建人类乳糖聚糖生物合成路径
Fuki Noda,Aika Ohno,Aruto Nakajima et al.
Fuki Noda et al.
Human milk oligosaccharides (HMOs) are complex sugars that play crucial roles in infant health by supporting the development of the gut microbiota and the immune system. HMOs are structurally based on lactose, which serves as the core disac...
A Plug-and-Play Cytochrome P450 System Enables De Novo Biosynthesis of 2-Phenylethanol Derivatives [0.03%]
一种即插即用的细胞色素P450系统可实现从头合成2-苯基乙醇衍生物
Yuanqing Wu,Yunxiao Wang,Yan Lv et al.
Yuanqing Wu et al.
The sustainable bioproduction of high-value aromatic compounds is a significant challenge in synthetic biology, often constrained by the complexity of multi-step metabolic pathways. To address this, we developed a cytochrome P450-driven plu...