Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 level [0.03%]
碳酐酶敲低提高雨生红球藻在高CO2水平下的生产力
Li Wei,Chen Shen,Mohamed El Hajjami et al.
Li Wei et al.
Improving acid tolerance is pivotal to the development of microalgal feedstock for converting flue gas to biomass or oils. In the industrial oleaginous microalga Nannochloropsis oceanica, transcript knockdown of a cytosolic carbonic anhydra...
Caleb Walker,Seunghyun Ryu,Cong T Trinh
Caleb Walker
Green organic solvents such as ionic liquids (ILs) have versatile use but are inhibitory to microbes even at low concentrations of 0.5-1.0% (v/v) ILs. We discovered the oleaginous yeast Yarrowia lipolytica can grow in 10% (v/v) of 1-ethyl-3...
Metabolic engineering of Chinese hamster ovary cells towards reduced biosynthesis and accumulation of novel growth inhibitors in fed-batch cultures [0.03%]
代谢工程改造中国仓鼠卵巢细胞以减少悬浮培养中未知生长抑制物质的生物合成和累积
Bhanu Chandra Mulukutla,Jeffrey Mitchell,Pauline Geoffroy et al.
Bhanu Chandra Mulukutla et al.
Chinese hamster ovary (CHO) cells in fed-batch cultures are known to consume large amounts of nutrients and divert significant portion of them towards the formation of byproducts, some of which, including lactate and ammonia, are known to b...
Balancing the non-linear rosmarinic acid biosynthetic pathway by modular co-culture engineering [0.03%]
模块化共培养工程技术用于平衡非线性路径的罗勒烯酸生物合成路径
Zhenghong Li,Xiaonan Wang,Haoran Zhang
Zhenghong Li
Pathway balancing is a critical and common challenge for microbial biosynthesis using metabolic engineering approaches. Non-linear biosynthetic pathways, such as diverging and converging pathways, are particularly difficult for bioproductio...
Annette Petersen,Christoph Crocoll,Barbara Ann Halkier
Annette Petersen
Microbial production of plant specialised metabolites is challenging as the biosynthetic pathways are often complex and can contain enzymes, which function is not supported in traditional production hosts. Glucosinolates are specialised met...
GC-MS-based 13C metabolic flux analysis resolves the parallel and cyclic glucose metabolism of Pseudomonas putida KT2440 and Pseudomonas aeruginosa PAO1 [0.03%]
基于GC-MS的13C代谢流分析解析了Pseudomonas putida KT2440和Pseudomonas aeruginosa PAO1中并行且循环的葡萄糖代谢
Michael Kohlstedt,Christoph Wittmann
Michael Kohlstedt
The genus Pseudomonas comprises approximately 200 species with numerous isolates that are common inhabitants of soil, water, and vegetation and has been of particular interest for more than one hundred years. Here, we present a novel approa...
Substrate promiscuity of enzymes from the sesquiterpene biosynthetic pathways from Artemisia annua and Tanacetum parthenium allows for novel combinatorial sesquiterpene production [0.03%]
来自黄花蒿和款冬属植物的倍半萜生物合成途径中的底物通用酶允许产生新的组合型倍半萜类化合物
Arman Beyraghdar Kashkooli,Alexander R van der Krol,Patrick Rabe et al.
Arman Beyraghdar Kashkooli et al.
The therapeutic properties of complex terpenes often depend on the stereochemistry of their functional groups. However, stereospecific chemical synthesis of terpenes is challenging. To overcome this challenge, metabolic engineering can be e...
Glycine decarboxylase regulates the maintenance and induction of pluripotency via metabolic control [0.03%]
丝氨酸脱羧酶通过代谢调节维持和诱导多能性
Phil Jun Kang,Jie Zheng,Gilju Lee et al.
Phil Jun Kang et al.
Reprogramming of 'adult' differentiated somatic cells to 'embryonic' pluripotent stem cells accompanied by increased rate of glycolysis. Conversely, glycolysis triggers accumulation of advanced glycation end products (AGEs), a potential cau...
Glyco-recoded Escherichia coli: Recombineering-based genome editing of native polysaccharide biosynthesis gene clusters [0.03%]
基因重组工程改造的糖基重构的大肠杆菌菌株：对天然多糖生物合成基因簇进行基因组编辑
Laura E Yates,Aravind Natarajan,Mingji Li et al.
Laura E Yates et al.
Recombineering-based redesign of bacterial genomes by adding, removing or editing large segments of genomic DNA is emerging as a powerful technique for expanding the range of functions that an organism can perform. Here, we describe a glyco...
Development of 3-hydroxypropionic-acid-tolerant strain of Escherichia coli W and role of minor global regulator yieP [0.03%]
具有3-羟基丙酸抗性的大肠杆菌W菌株的开发及其全局调控因子yieP的作用研究
Thuan Phu Nguyen-Vo,Yunxiao Liang,Mugesh Sankaranarayanan et al.
Thuan Phu Nguyen-Vo et al.
3-Hydroxypropionic acid (3-HP) is an important platform chemical, but its toxic effect at high concentrations (> 200 mM) is a serious challenge for commercial production. In this study, a highly 3-HP-tolerant strain of Escherichia coli W (t...