Heterodimeric aptamer improves the limit of detection of sandwich ELASA and dot blot assay [0.03%]
异源二聚体适配子可提高夹心ELISA和点印迹法的检出限
Tzi Shien Yeoh,Marimuthu Citartan
Tzi Shien Yeoh
Heterodimerization, which is an amalgamation of discrete aptamers into a single functional module is a viable strategy to augment the binding strength of aptamers. In this study, we sought to derive several heterodimeric aptamers using the ...
Munem Shahriar Shoyshob,Kusay Faisal Al-Tabatabaie,Lway Faisal Abdulrazak et al.
Munem Shahriar Shoyshob et al.
Autophagy is an important cell process that may be critical for various physiological activities as well as maintenance of the cellular bioenergetic and metabolic homeostasis. Identifying the proteins involved in autophagy is essential for ...
High-Throughput Protein A Chromatography Platform for Accurate Antibody Quantification in IV Admixtures [0.03%]
高效蛋白质A亲和色谱平台在静脉滴注混合物中准确定量抗体的能力
Tse-Hong Chen,Xiaoyang Liu,Shao-Chun Wang et al.
Tse-Hong Chen et al.
Antibodies hold significant therapeutic potential, but accurate quantification of them in complex intravenous (IV) admixtures is critical for ensuring therapeutic efficacy and patient safety. Traditional reversed-phase liquid chromatography...
Real-Time Electrochemical Detection of Ascorbic Acid in Mouse Brain Homogenate Using SCN-Wrapped Co@NiO Nanosensor [0.03%]
基于包裹SCN的Co@NiO纳米传感器实时检测鼠脑匀浆中的抗坏血酸
Shahid Habib Ansari,Sana Amjad,Tehreem Ul Wara et al.
Shahid Habib Ansari et al.
Oxidative stress, a major key factor to neurological disorders such as Parkinson's, Alzheimer's, and Huntington's disease. Ascorbic acid (AA), a vital brain antioxidant, protects neurons by scavenging reactive oxygen species, and its fluctu...
qPCR-based method to measure plasmid internalization efficiency of non-viral nanoparticle carriers in adherent cell model [0.03%]
一种基于qPCR的方法，用于测量非病毒纳米颗粒载体在贴壁细胞模型中质粒内化的效率
Priscila Tonon Baschirotto,Samara Valeria López Ramírez,Randall Loaiza-Montoya
Priscila Tonon Baschirotto
Non-viral nanoparticle-mediated transfection systems have gained wide acceptance in modern pharmaceutical and biotechnological applications, including RNA delivery for COVID-19 vaccines. However, these systems may show low transfection and ...
Biosynthesised reduced graphene oxide/CuO based nanocomposite using 'Cordia dichotoma' leaf extract for 'nitrobenzene' determination [0.03%]
采用 Cordia dichotoma 叶提取物生物合成还原氧化石墨烯/铜复合纳米材料以检测硝基苯
Devkumari Patel,Robind Kumar,Sanju Yadav et al.
Devkumari Patel et al.
Herein, plant-assisted synthesis of copper oxide nanoparticles (CuO NPs)CD and reduced graphene oxide (CDrGO) was performed individually using the leaf extract of 'Cordia dichotoma' plant and they were associated with electrochemically acti...
Mapping transposon insertion sites within bacterial genomes by direct Sanger sequencing [0.03%]
直接Sanger测序法绘制细菌基因组中的转座元件插入位点
Scott W Herke,Linda M Heffernan,William N Beavers et al.
Scott W Herke et al.
Microbial biomedical research frequently involves mutagenesis by insertion of transposons into the genome. Currently, transposon insertion locations are typically elucidated by Next Generation Sequencing or by Sanger sequencing of PCR produ...
Easy reference-guided assembly of nanopore whole plasmid sequencing datasets [0.03%]
基于简单参考基因组的纳米孔质粒测序数据拼接组装
Vinita Sharma,Naiyu Jiang,Yukihiro Nagashima et al.
Vinita Sharma et al.
Whole plasmid sequencing (WPS) using Nanopore long read sequencing has emerged as a cost-effective alternative for dideoxy sequencing methods. De novo sequence assembly for large plasmids, however, are not always successful and may produce ...
PreRBP: Interpretable Deep Learning for RNA-Protein Binding Site Prediction with Attention Mechanism [0.03%]
基于注意力机制的RNA-蛋白质结合位点预测的可解释深度学习方法
Huixian Chen,Yun Zuo,Xiangrong Liu et al.
Huixian Chen et al.
In the complex process of gene expression and regulation, RNA-binding proteins occupy a pivotal position for RNA. Accurate prediction of RNA-protein binding sites can help researchers better understand RNA-binding proteins and their related...
Exploration of endoplasmic reticulum stress-related gene markers in amyotrophic lateral sclerosis: a comprehensive analysis of bioinformatics and machine learning [0.03%]
基于生物信息学和机器学习的肌萎缩侧索硬化内质网应激相关基因标志物研究
Jing Wang,Xinmin Li,Fangjie Yang et al.
Jing Wang et al.
This study aimed to investigate potential biomarkers related to Endoplasmic reticulum (ER) stress in Amyotrophic lateral sclerosis (ALS) through a comprehensive bioinformatic approach. The gene expression profiles of ALS patients and health...