Aging differentially affects the uptake of methionine by select rat tissues [0.03%]
老龄化对大鼠选择性组织甲硫氨酸摄取的的影响不同
Juan A Azcona,Anja S Wacker,Thomas M Jeitner et al.
Juan A Azcona et al.
Experimental and epidemiological studies indicate that significant reductions in dietary methionine intake profoundly impact both health and life spans. Methionine levels also decrease in the blood of mammals as they age. Here, we report th...
Comparing digital and real-time PCR platforms for detecting residual iPSCs and virus-producing cells in manufacturing [0.03%]
用于制造过程中检测残留的iPSC和病毒生成细胞的数字PCR与实时PCR平台比较研究
Un Na Koh,Si-Keun Lim
Un Na Koh
Digital PCR (dPCR) enables absolute nucleic acid quantification and has been widely adopted for quality control (QC) applications in cell therapy manufacturing. Ensuring patient safety during cell therapy manufacturing requires reliable det...
Engineering GFP-NanoLuc Fusion Substrates for Sensitive, Quantitative Detection of Protease Activity via BRET [0.03%]
基于生物发光共振能量转移的蛋白酶活性定量检测新技术
Mitsuki Nakamura,Masafumi Sakono
Mitsuki Nakamura
Proteases play essential roles in diverse biological processes and are closely associated with various diseases, making them important targets for diagnostics and therapeutic development. Conventional protease activity assays often rely on ...
Establishing the Plasma Proteomic Baseline of Crotalus durissus terrificus for Health Monitoring [0.03%]
基于Crotalus durissus terrificus的等离子蛋白基线建立及其在健康监测中的应用研究
Isabela Tokuhara Makida,Amanda Almeida Resende,Letícia Gomes de Pontes et al.
Isabela Tokuhara Makida et al.
Snakebite envenoming remains a major public health concern worldwide, while the maintenance of captive snakes is essential for conservation, venom production, and biomedical research. However, little is known about the plasma proteome of sn...
Fluorescence-based assay for rapid screening of GABAA receptor modulating steroid antagonists (GAMSA) [0.03%]
基于荧光的快速筛选γ-氨基丁酸A型受体调节类固醇拮抗剂(GAMSA)的试验方法
Jan Voldřich,Hana Zemková,Markéta Šmídková et al.
Jan Voldřich et al.
GABAA receptor modulating steroid antagonists (GAMSA) represent a promising therapeutic class by selectively inhibiting the effects of positive allosteric modulators (PAMs) at GABAA receptors without affecting GABA-evoked currents alone. Th...
Oxidative interference in immunoturbidimetric assays for the quantification of immunoglobulins and complement proteins [0.03%]
氧化干扰在免疫浊度法测定免疫球蛋白和补体蛋白定量中的作用
Bruna Garlet Rossato,Rafael Noal Moresco
Bruna Garlet Rossato
Immunoturbidimetric assays are widely used to quantify immunoglobulins and complement proteins but are susceptible to interference. This study evaluated the effects of oxidative agents, including the Fenton reaction and hypochlorous acid (H...
Recent advances in ZnO-based electrochemical sensors for food safety monitoring [0.03%]
基于ZnO的食品传感检测用电化学传感器研究进展
Md Maruf Ahmed,Qin Xu
Md Maruf Ahmed
Food safety is a paramount worldwide issue that directly affects human health and well-being, underscoring the necessity for rapid and accurate detection strategies. Conventional analytical techniques, including chromatography, mass spectro...
FTIR Spectroscopy Combined with Machine Learning Reveals Molecular Signatures Distinguishing three Phenotypes of Endometriosis [0.03%]
基于傅里叶变换红外光谱结合机器学习鉴别三种子宫内膜异位症表型的分子标志研究
Piotr Olcha,Wiesław Paja,Michał Kępski et al.
Piotr Olcha et al.
Endometriosis is a chronic inflammatory disorder in which endometrial tissue grows outside the uterus, leading to pelvic pain and infertility. It remains a major challenge in women's health due to delayed diagnosis and limited therapeutic o...
A high-performance PdO@SWCNTs/NiO nanocomposite-based electrochemical sensor for sensitive and selective detection of hydroquinone [0.03%]
一种高性能的PdO@SWCNTs/NiO纳米复合材料基电化学传感器用于对间苯二酚的选择性灵敏检测
M Sabbir Hossain,M Hafizur Rahman,Md A Rashed et al.
M Sabbir Hossain et al.
In this study, a highly sensitive electrochemical sensor with low cost is designed for determining Hydroquinone (HQ) by modifying glassy carbon (GC) electrodes with PdO@SWCNTs/NiO nanocomposite. Herein, a semiconductor metal oxide (NiO) is ...
Mining bacterial (meta)genomes for enzymes active in aerobic, mesophilic conditions [0.03%]
挖掘细菌基因组和宏基因组以获得在好氧温和条件下的活性酶
Rodrigo Campos-Silva,Fahimeh Rahimi,Jaya Joshi et al.
Rodrigo Campos-Silva et al.
Enzyme biochemistry can now draw on hundreds of thousands of prokaryotic genomes and metagenomes to identify orthologous genes for research, biocatalysis, and metabolic engineering. In many applications, adaptation to O2 and mild temperatur...