Wei Wang,Qing Chen
Wei Wang
FcγR binding characterization is one of the critical attributes during the development of therapeutic antibodies. Here, we report a novel assay format to characterize IgG-FcγR interaction in the presence of antigen using Surface plasmon r...
Simultaneous quantification of 26 NAD-related metabolites in plasma, blood, and liver tissue using UHPLC-MS/MS [0.03%]
使用UHPLC-MS/MS同时量化血浆、血液和肝组织中26种与NAD相关的代谢物
Hartmut Cuny,Esther Kristianto,Mark P Hodson et al.
Hartmut Cuny et al.
Nicotinamide adenine dinucleotide (NAD) is a key metabolic intermediate found in all cells and involved in numerous cellular functions. Perturbances in the NAD metabolome are linked to various diseases such as diabetes and schizophrenia, an...
Quantitation of thrombin-activatable fibrinolysis inhibitor in human plasma by isotope dilution mass spectrometry [0.03%]
同位素稀释质谱法定量人血浆中的凝血酶活化纤溶抑制因子
Jun X Wheeler,Craig Thelwell,Peter Rigsby et al.
Jun X Wheeler et al.
Measurement of Thrombin-activatable fibrinolysis inhibitor (TAFI) in human plasma is dependent on reproducible assays. To date, standards for measuring TAFI are frequently calibrated relative to pooled normal human plasma and arbitrarily as...
Fifteen years of urea cycle disorders brain research: Looking back, looking forward [0.03%]
十五年尿素循环障碍脑研究的回顾与展望
Kuntal Sen,Matthew Whitehead,Carlos Castillo Pinto et al.
Kuntal Sen et al.
Urea cycle disorders (UCD) are inherited diseases resulting from deficiency in one of six enzymes or two carriers that are required to remove ammonia from the body. UCD may be associated with neurological damage encompassing a spectrum from...
Pharmacokinetic and excretion study of eight active constituents in rat by LC-MS/MS after oral administration of the Toddalia asiatica extract [0.03%]
口服亚洲雀瓢藤提取物后大鼠体内8种主要活性成分的药代动力学及排泄研究（利用LC-MS/MS法）
Jingwen Qiu,Mingjuan Zhu,Ying Wang et al.
Jingwen Qiu et al.
Toddalia asiatica L., a significant medicinal plant in the family Rutaceae, has been applied to treat rheumatoid diseases for decades. Its pharmacological activities are mainly attributed to the existence of generous coumarins and alkaloids...
Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant [0.03%]
使用耐盐突变型荧光素酶改良的基于生物发光的内毒素检测方法
Satoshi Yawata,Kenichi Noda,Ai Shimomura et al.
Satoshi Yawata et al.
Endotoxin is a component of the cell wall of gram-negative bacteria and causes fever and shock symptoms upon entering the bloodstream. We previously demonstrated that the bioluminescence-based Limulus amebocyte lysate test is highly sensiti...
Determination of the SLAMF1 self-association affinity constant with sedimentation velocity ultracentrifugation [0.03%]
使用沉降速度超速离心法确定SLAMF1自关联亲和常数
Yangjie Wei,JangEun Lee,Michael Dziegelewski et al.
Yangjie Wei et al.
Signaling lymphocytic activating molecule family member 1 (SLAMF1 or CD150) is a cell surface glycoprotein expressed on various immune populations, regulating cell-cell interactions, activation, differentiation, and inflammatory responses a...
Fluorimetric method for specific detection of human serum albumin in urine using its pseudoesterase property [0.03%]
利用pseudoesterase属性在尿液中特异性检测人血清白蛋白的荧光测定法
Deepak Kumar,Rajasri Bhattacharyya,Dibyajyoti Banerjee
Deepak Kumar
Detection of microalbuminuria is an analytical challenge. There are dye-based methods and immunochemical methods. However, these methods are less specific and sensitive respectively. So, people are trying new approaches for microalbuminuria...
Biosensors, microfluidics systems and lateral flow assays for circulating microRNA detection: A review [0.03%]
循环microRNA检测用的生物传感器，微流体系统和侧向流动试验：一篇综述
Niloofar Khandan-Nasab,Saeedeh Askarian,Arash Mohammadinejad et al.
Niloofar Khandan-Nasab et al.
MicroRNAs (miRNAs) are short RNA sequences found in eukaryotic cells and they are involved in several diseases pathogenesis including different types of cancers, metabolic and cardiovascular disorders. Thus, miRNAs circulating in serum, pla...
Antibody orientational labeling via staphylococcus A protein to improve the sensitivity of gold immunochromatography assays [0.03%]
通过葡萄球蛋白A对抗体进行定向标记以提高金免疫层析检测的灵敏度
Penghua Zhao,Xiaoyan Huang,Haiqiang Tao et al.
Penghua Zhao et al.
The Cry1Ab toxin is usually expressed in genetically modified crops in order to control chewing pests. Although the gold immunochromatography assay (GICA) based on the double-antibody sandwich method has been developed to detect this toxin,...