Partial purification and characterization acidophilic lipase from Bacillus cereus ALP E1 [0.03%]
来源于Bacillus cereus ALP E1嗜酸性脂酶的部分纯化和性质分析
Nindy Novita Sari,Nurhasanah,Titin Haryati
Nindy Novita Sari
The discovery of a new microbial lipase is crucial since its utilization has various industrial applications. Bacillus cereus is a strain that has emerged as a potential source of microbial lipase. Until now, the exploration of new lipases ...
Generation of a polyclonal antibody that specifically recognizes the recombinant NS5A protein of bovine viral diarrhea virus [0.03%]
一种能特异识别牛病毒性腹泻病毒重组NS5A蛋白的多克隆抗体的制备
Weijie Zhou,Yuting Wang,Shaobo Liang et al.
Weijie Zhou et al.
Bovine viral diarrhea virus (BVDV) is an economically important pathogen for causing bovine viral diarrhea (BVD), with a significant detrimental impact on cattle production worldwide. While the BVDV NS5A protein is known to be essential for...
Isolation, purification and identification of major allergens Cor a 9 and Cor a 14 in hazelnuts [0.03%]
隔离、提纯和鉴定导致榛子过敏反应的主要过敏原Cor a 9和Cor a 14蛋白质
Weichao Zhu,Huahong Yu,Ying Zhang et al.
Weichao Zhu et al.
Hazelnut (Corylus avellana) is a widely consumed nut with potent allergenic potential, and the isolation and purification of native hazelnut allergens are critical for the effective prevention and management of hazelnut allergy. In this stu...
Optimized Production and Coagulation Activity of rhFVII in HEK293 Cells for Bleeding Disorders [0.03%]
人HEK293细胞中rhFVII的优化生产和凝血作用研究用于止血 disorders
Sen Zou,Xiaoxiao Li,Jiajun Liu et al.
Sen Zou et al.
Hemophilia, a genetic disorder characterized by impaired blood clotting, necessitates innovative treatments. This study optimized the expression of recombinant human coagulation factor VII (rhFVII) in HEK293 cells to enhance its therapeutic...
Characterization of a novel GH5 β-mannanase from Hahella sp. CR1 and its synergistic role in degradation of spent coffee grounds [0.03%]
来自Hahella sp. CR1的新型GH5型β-甘露聚糖酶的表征及其在废弃咖啡渣降解中的协同作用
Melvin Chun Yun Tan,Kok Jun Liew,Faezah Mohd Salleh et al.
Melvin Chun Yun Tan et al.
An efficient enzymatic degradation of plant biomass is essential for agricultural waste valorization and biofuel production. β-Mannanase is a key lignocellulolytic enzyme that targets mannan, a major component in plant cell walls. Hahella ...
Analysis of the antibacterial and anti-inflammatory functions of bovine lactoferrin functional fragment expressed in Escherichia coli via engineering [0.03%]
工程改造的大肠杆菌表达乳铁蛋白功能片段的抗菌抗炎活性分析
Hui Teng,Zizi Ding,Yujie Wang et al.
Hui Teng et al.
The bovine lactoferrin is a natural iron-binding glycoprotein that has attracted widespread attention due to its functions such as antibacterial, antiviral, anti-tumor, anti-inflammatory and immunomodulatory effects. In this study, the lact...
Systematic optimization for high-level secretory expression of a thermostable zearalenone hydrolase ZENM in Pichia pastoris [0.03%]
毕赤酵母中高效分泌表达耐热性玉米赤霉烯酮水解酶ZENM的系统优化
Shida Zhao,Qin Zhou,Yuzhuo Wu et al.
Shida Zhao et al.
Zearalenone (ZEN), a mycotoxin commonly found in cereal grains, poses a significant threat to livestock health. ZEN hydrolase is considered a key tool for detoxifying ZEN contamination due to its high efficiency, cost-effectiveness, and abi...
Codon harmonization for improve heterologous expression of human aromatase (CYP19A1) in E. coli cells [0.03%]
通过密码子优化改善大肠杆菌中人源芳香酶(CYP19A1)的异源表达
Marina I Shaladonova,Yaraslau U Dzichenka,Veronika V Shchur et al.
Marina I Shaladonova et al.
CYP19A1 (aromatase) is the key enzyme taking part in the process of the conversion of androgens to estrogens thus considering as a major target for treatment of certain types of tumors, especially hormone dependent breast cancer. The purpos...
Expression, Purification, and Biophysical Characterization of Liquid-Liquid Phase Separation of Full-Length hnRNPA2B1 [0.03%]
全长hnRNPA2B1的表达、纯化及液-液相分离生物物理表征
Luis Fernando Durán-Armenta,Attila Meszaros,Julia Malo Pueyo et al.
Luis Fernando Durán-Armenta et al.
Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1) is a multifunctional RNA-binding protein involved in RNA maturation and mRNA transport. It has recently been shown to undergo liquid-liquid phase separation (LLPS), contributing to t...
Synergistic effect of LTB and Mn2+ adjuvants on immunogenicity of multi-epitope peptides from Staphylococcus aureus [0.03%]
LTB和Mn2+佐剂对金黄色葡萄球菌多表位肽免疫原性的影响研究
Jinzhu Ma,Shuyu Wei,Ming Xu et al.
Jinzhu Ma et al.
Here, a novel combined adjuvant of heat-labile enterotoxin B (LTB) as an intramolecular adjuvant plus Mn2+ was prepared to enhance the immunogenicity of multi-epitope (MEP) peptides derived from Staphylococcus aureus (S. aureus). Initially,...