Scalable insect cell expression and purification screening applied to CRL4-DCAF substrate receptors [0.03%]
应用于CRL4-DCAF底物受体的昆虫细胞表达和纯化筛选方法学研究
Angus D Cowan,Stefan Jaekel,Alessio Ciulli
Angus D Cowan
The ubiquitin-proteasome system is one of the primary mechanisms responsible for degradation of intracellular proteins. Cullin-RING E3 ligases (CRL) are modular, multi-subunit complexes that catalyse ubiquitination of a wide variety of prot...
Expression, purification and biochemical characterization of the periplasmic nitrate reductase NapA from Magnetospirillum gryphiswaldense MSR-1 [0.03%]
来源于磁小体螺旋菌MSR-1的周质硝酸盐呼吸酶NapA的表达、纯化及生化性质表征
Chenchen Xu,Hengjia Wan,Yaoyao Zhang et al.
Chenchen Xu et al.
Magnetospirillum gryphiswaldense MSR-1 is a model magnetotactic bacterium whose magnetosome biomineralization is closely linked to intracellular redox regulation. Although the periplasmic nitrate reductase NapA plays an essential role in de...
Protein expression control by targeted elimination of ovomucoid in chicken eggs [0.03%]
通过靶向去除鸡卵中的卵黏蛋白进行蛋白质表达控制
Ryo Ezaki,Hiroyuki Horiuchi
Ryo Ezaki
Ovomucoid is a major allergen in chicken egg whites and is characterized by exceptional structural stability conferred by multiple disulfide bonds and glycosylation, rendering it highly resistant to thermal processing and gastrointestinal d...
A novel flavin-containing monooxygenase from Pseudomonas guineae enables efficient biosynthesis of indigo from indole via both enzymatic and cell factory approaches [0.03%]
一种来自假单胞菌的新黄素单加氧酶通过酶法和细胞工厂实现了吲哚高效合成靛蓝
Zhebin Hao,Yulei Zhang,Wenli Zhang et al.
Zhebin Hao et al.
Indigo is an important blue pigment widely used in textile, food, and pharmaceutical industries. Currently, most of indigo was produced via chemical synthesis, which led to pollution to the environment and potential health hazards to consum...
Exploring Elastin-Like Polypeptide Tags and Mini-intein for Recombinant Protein Purification in Leishmania tarentolae [0.03%]
利用Leishmania tarentolae探索弹性蛋白多肽标签和mini-intein重组蛋白质纯化新技术
Lucia Bassetti,Martina Crispo,Mariela Bollati-Fogolin et al.
Lucia Bassetti et al.
Recombinant technology has enabled the production of a wide range of proteins with applications in various fields, including reproduction, food supplements, and medicine. However, the high costs associated with producing and purifying recom...
Corrigendum to "Catalytically active hCELA3B is a natively-folded monomer and is N-glycosylated" [Protein Expr. Purif. 238, (2026), 106840] [0.03%]
“催化活性hCELA3B是以天然折叠的单体形式存在且发生N-糖基化的”一文的勘误(蛋白表达与纯化第238卷(2021)篇106840)
Prince Kumar,Prabhakar Babele,Rishav Madhukalya et al.
Prince Kumar et al.
Published Erratum
Protein expression and purification. 2026 Feb 19:106899. DOI:10.1016/j.pep.2026.106899 2026
Sterile chicken hosts for germline transmission: A review of recipient preparation strategies in avian biotechnology [0.03%]
用于遗传操作的无菌鸡宿主：禽类生物技术中受体准备策略的综述
Yi-Chen Chen,Tatsuya Takemoto
Yi-Chen Chen
The chicken (Gallusgallus) has long served as a vital model in developmental biology and agriculture. Recent advancements in genome editing, particularly through germ cell-mediated techniques, have significantly enhanced the generation of t...
Strategies for manufacturing soluble human alphainterferons in Escherichia coli: expression, purification, and testing [0.03%]
在大肠杆菌中制造可溶性人白细胞介素干扰素的策略：表达、纯化和测定
Rodrigo Martins Bretas,Sophie Yvette Leclercq,Armando da Silva Cunha Jr et al.
Rodrigo Martins Bretas et al.
Escherichia coli remains the preferred recombinant expression host due to its well-characterized genetics and high-yield biosynthetic capacity, especially when dealing with biopharmaceuticals without complex post-translational modifications...
Pharmaceutical protein production by transgenic chickens: several viewpoints towards the next stage [0.03%]
转基因鸡制药蛋白生产：面向下一阶段的若干观点
Ken-Ichi Nishijima,Yuya Okuzaki
Ken-Ichi Nishijima
Several pharmaceuticals produced by transgenic chickens, Kanuma and Epovet, are already in commercial use, which indicates the potential cost competitiveness of this approach as an "animal bioreactor." Since the establishment of reliable cu...
A Self-Cleavable ULP1-SUMO Fusion Strategy for Enhanced Soluble Protein Expression and One-Step Purification in Escherichia coli [0.03%]
一种自我裂解的ULP1-SUMO融合策略，可增强大肠杆菌中的可溶性蛋白质表达和一步纯化
Changbin Jin,Xiaohu Zhang,Zhibo Yang et al.
Changbin Jin et al.
The Escherichia coli (E. coli) expression system is widely used for recombinant protein production; however, protein insolubility remains a major challenge. Although fusion tags can enhance solubility, they often complicate purification due...