Highly Proliferative Silkworm Cell Line: Development of a New Line for Recombinant Protein Production [0.03%]
高增殖的家蚕细胞系：一种新的重组蛋白生产细胞系的发展
Abubakar Nura,Jae Man Lee,Akitsu Masuda et al.
Abubakar Nura et al.
Recombinant proteins such as vaccines, antibodies, and enzymes play essential roles in research, veterinary, and medical applications. The insect cell-baculovirus expression system is widely used for recombinant protein production, and silk...
Corrigendum to "Molecular chaperones and solubility tags: A 44-fold increase in recombinant fibEase yield for therapeutic applications" [Protein Expr. Purif. 241 (2026) 106929] [0.03%]
关于“分子伴侣和溶解度标签：用于治疗应用的重组fibEase产量提高44倍”[蛋白质表达与纯化241（2026）106929]的勘误表
Zhiqun Che,Suyuan Wei,Renjie Sun et al.
Zhiqun Che et al.
Published Erratum
Protein expression and purification. 2026 May 9:106949. DOI:10.1016/j.pep.2026.106949 2026
Comparative structural analysis of Nipah virus V proteins highlights V-MDA5 complex stability in pig and bat isolates [0.03%]
猪和蝙蝠尼帕病毒V蛋白的结构比较分析揭示了V-MDA5复合物的稳定性
Chee Ning Chong,Hui Ming Ong,Yvonne Jing Mei Liew et al.
Chee Ning Chong et al.
Nipah virus (NiV) V protein antagonizes host interferon (IFN) signaling by targeting melanoma-differentiation association protein 5 (MDA5). This study investigated structural features of NiV V proteins from pig (iP-V) and bat (iB-V) isolate...
Purification and characterization of rabbit polyclonal immunoglobulin G using DEAE ion-exchange chromatography and dynamic light scattering [0.03%]
采用DEAE离子交换色谱法和动态光散射法纯化并表征兔多克隆免疫球蛋白G
S H S Mariam,Roslan Noorain,W S Tan et al.
S H S Mariam et al.
Weak anion-exchange chromatography (AEC) provides a mild alternative to ammonium sulfate (AS) precipitation for antibody purification, but its mechanistic behavior in complex serum systems remains unclear. This study evaluates pH-dependent ...
Identification and characterization of WpDestabilase, a novel destabilase from the salivary glands of Whitmania pigra [0.03%]
identification and characterization of wpdestabilase，a novel destabilase from the salivary glands of whitmania pigra
Bo Huang,Tianyi Gao,Yueyue Mei et al.
Bo Huang et al.
Whitmania pigra is one of the primary sources of leeches used in traditional Chinese medicine, renowned for its anticoagulant and thrombolytic properties. In this study, a salivary gland transcriptome database of W. pigra was constructed, a...
Enhanced large-scale production and purification of recombinant human NUPR1 for detailed structural and functional insights [0.03%]
增强型重组人NUPR1的大规模生产和纯化以深入了解其结构和功能
Minsu Kim,Joonhyeok Choi,Hae-Kap Cheong et al.
Minsu Kim et al.
Nuclear Protein 1 (NUPR1) is a critical modulator of numerous cellular processes, including cell cycle regulation, apoptosis, and oncogenic transformation. Its structural characterization is indispensable for understanding its multifaceted ...
Extraction, purification and characterization of lipoxygenase from foxtail millet (Setaria italica) [0.03%]
从狗尾草中提取、纯化及鉴定脂氧合酶
Chen Li,Zonghua Tan,Jiao Li et al.
Chen Li et al.
This study established a method for extracting and purifying foxtail millet lipoxygenase (LOX) by combining ammonium sulfate precipitation and ion exchange chromatography, and investigated its enzymatic properties. The purified LOX showed h...
SUMO-assisted expression of a soluble and functional Venus flytrap domain of the human sweet taste receptor TAS1R2 in Escherichiacoli [0.03%]
用于大肠杆菌表达的人类甜味受体TAS1R2的伏勒吞捕虫器结构域的SUMO表达系统及其应用
Christine Belloir,Adeline Karolkowski,Lucie Moitrier et al.
Christine Belloir et al.
The heterodimeric human sweet taste receptor TAS1R2/TAS1R3 is a class C G-protein-coupled receptor responsible for detecting a wide range of sweet-tasting compounds. The Venus flytrap domain of hTAS1R2 (hTAS1R2-VFT) constitutes the main bin...
Identification and characterization of a novel lysin-like endopeptidase from the Vibrio cholerae predator phage ICP1 [0.03%]
来源于霍乱弧菌裂解性病毒ICP1的新型溶胞酶类内肽酶的鉴定及表征
Laura Espinosa-Barrera,Oscar Linares-Vergara,Lina A Zermeño-Cervantes et al.
Laura Espinosa-Barrera et al.
International Centre for Diarrheal Disease Research, Bangladesh cholera phage 1 (ICP1)-type vibriophages are specialized predators of the Gram-negative (G-) pathogen Vibrio cholerae. However, the muralytic lysins critical to the lytic mecha...
Fusion tags for recombinant protein expression and purification in structural and functional studies [0.03%]
结构与功能研究中融合标签在重组蛋白表达及纯化中的应用
Qingxin Li
Qingxin Li
Protein purification is a critical prerequisite for biochemical, biophysical, and structural analyses. Although proteins can be isolated from their native environments, this approach is often limited by low yield and heterogeneity. Recombin...