Comparative analysis of charge heterogeneity in anti-sclerostin monoclonal antibodies produced via continuous perfusion and fed-batch processes [0.03%]
连续灌流和补料批式培养过程产生的抗成骨素单克隆抗体电荷异构体差异分析
Yu Yi,Dawei Xie,Xichen Zhang et al.
Yu Yi et al.
As critical quality attributes, post-translational modifications (PTMs) of therapeutic monoclonal antibodies in production impact their structural stability, charge heterogeneity, and clinical efficacy. This study elucidated PTM-driven char...
Expression, purification, and preliminary cryo-EM analysis of the human urotensin 2 receptor/urotensin 2 complex [0.03%]
人尿囊素2受体/尿囊素2复合物的表达、纯化及初步冷冻电镜分析
Seungbeen Koh,Minhyuk Park,Da Jin Jung et al.
Seungbeen Koh et al.
Human urotensin 2 (hU2) interacts with the urotensin 2 receptor (UR2R), a member of the G protein-coupled receptor (GPCR) family. UR2R signaling is known to induce vasoconstriction and promote cell proliferation, and its overexpression is s...
Engineering of dual-responsive elastin-like polypeptide (ELP) systems for precision delivery of calcitonin gene-related peptide (CGRP): Impact of chain length on performanc [0.03%]
工程制备双响应性弹性蛋白多肽（ELP）系统用于精准递送降钙素基因相关肽（CGRP）：重复序列链长对性能的影响
Qi Zhao,Lei Yang,Bowen Pang et al.
Qi Zhao et al.
Background: Calcitonin gene-related peptide (CGRP) holds significant potential for tissue regeneration and bone repair; however, its clinical application is limited by a rapid systemic half-life and the difficulty of main...
Recombinant expression and purification of a hybrid antimicrobial peptide Aur-Defb3 in Komagataellaphaffii [0.03%]
毕赤酵母中抗菌肽Aur-Defb3融合蛋白的表达及纯化
Jiali Cai,Haimei Wang,Meiqi Li et al.
Jiali Cai et al.
Antimicrobial peptides (AMPs) are promising alternatives to conventional antibiotics against multidrug-resistant pathogens, but their application is limited by low yield, structural instability, and potential toxicity. In this study, we des...
Cloning, heterologous expression, and polyclonal antibody generation against the macrophage mannose receptor gene of Pierisrapae [0.03%]
小菜蛾巨噬细胞甘露糖受体基因的克隆、异源表达及多克隆抗体构建
Jing Chen,Lingzhi Tang,Hongbo Li
Jing Chen
The macrophage mannose receptor (MMR) is an important member of the C-type lectin family. It plays a significant role in recognizing pathogen-associated glycans and mediating immune phagocytosis. However, its specific function in insect imm...
Oxidative Refolding and NMR Analysis of the Second Extracellular Loop of Human Occludin: The C216-C237 Disulfide Bond as a Structural Linchpin [0.03%]
人occludin的第二胞外环的氧化复性及核磁共振分析：C216-C237二硫键作为结构支柱的作用
Hengzhi Cai,Zewen Zhuang,Xueyan Guan et al.
Hengzhi Cai et al.
Occludin (OCLN) is a key integral membrane protein of tight junctions, essential for maintaining the blood-tissue barrier and serving as a critical co-receptor for Hepatitis C Virus entry. Its second extracellular loop (EL2) is a primary de...
Heterologous Expression, Refolding, and Functional Characterization of Halocin C8 from Halophilic Archaea in Escherichia coli [0.03%]
四膜虫i型telomerase逆转录酶的人工进化研究
Hongjing Xing,Boyuan Sun,Hanyu Li et al.
Hongjing Xing et al.
Halocins are antimicrobial peptides secreted by halophilic archaea, stable under hypersaline conditions and promising for food preservation and biomedical fields. In this study, bioinformatic analysis revealed that the halC8 coding region o...
Rapid expression of pyruvate decarboxylase from Zymomonas mobilis in E. coli BL21 LysY/Iq [0.03%]
来源于Zymomonas mobilis的丙酮酸脱羧酶在E.coli BL21 LysY/Iq中的快速表达
S Bilal Jilani,Daniel G Olson
S Bilal Jilani
E. coli BL21 DE3 strain is commonly used to purify non-toxic prokaryotic proteins. Traditionally, IPTG based induction of the host strain at reduced temperatures for extended period (12-16 h) is performed to obtain high yield of functional ...
High yield production of C-terminally processed KRAS4a, HRAS, and NRAS for biophysical study [0.03%]
用于生物物理研究的KRAS4a、HRAS和NRAS C端加工高产生成方法
Shelley Perkins,Sophie Krahnke,Erik K Larsen et al.
Shelley Perkins et al.
The classical RAS family of proteins consist of four isoforms HRAS, KRAS4a, KRAS4b, and NRAS, mutations in which lead to several types of human cancer, including pancreatic, colorectal, lung, and melanoma. RAS proteins function as molecular...
Recombinant indoleamine 2,3-dioxygenase as a reliable alternative to HRP with enhanced cumulative output in immunoassays [0.03%]
重组吲哚胺2,3-双加氧酶作为HRP在免疫测定中更可靠的替代品并具有增强的累积输出量
Liru Liu,Sheng Ye
Liru Liu
Horseradish peroxidase (HRP) is the most widely used reporter in immunoassays, yet its recombinant production and the heterogeneity of isoforms derived from plants impose constraints and practical inconveniences on its application. Here, we...