Expression and purification of recombinant human insulin from E. coli 20 strain [0.03%]
大肠杆菌20菌株中重组人胰岛素的表达及纯化
Marcin Zieliński,Agnieszka Romanik-Chruścielewska,Diana Mikiewicz et al.
Marcin Zieliński et al.
The number of people with diabetes is estimated to be over 370 million, in 2030 it will increase to 552 million. In Poland, the number of people with diabetes is estimated to be 3.5 million (9.1%). According to the estimates of the Internat...
Preparation and characterization of humanized nanobodies targeting the dimer interface of epidermal growth factor receptor (EGFR) [0.03%]
人源化纳米抗体的制备与表征：靶向表皮生长因子受体(EGFR)二聚体界面
He Zhu,Lin Zhao,Zemin Li et al.
He Zhu et al.
Epidermal growth factor receptor (EGFR) is an effective target for the treatment of many epithelial cancers. However, EGFR inhibitors have low clinical response rates and are prone to drug resistance arising from mutations and heterodimeriz...
Hanmei Li,Zeeshan Ali,Xiaolong Liu et al.
Hanmei Li et al.
The development of antibiotic-resistant bacteria has become a major public health problem, prompting the search for alternative solutions. Tachyplesin I (TP-I) is an antimicrobial peptide, which exhibits potent and broad-spectrum activities...
Expression of glyceraldehyde-3-phosphate dehydrogenase from M. tuberculosis in E. coli. Purification and characteristics of the untagged recombinant enzyme [0.03%]
大肠杆菌中表达的结核分枝杆菌丙糖磷酸脱氢酶的纯化及性质研究
E V Schmalhausen,M S Shumkov,V I Muronetz et al.
E V Schmalhausen et al.
The goal of the present work was to produce glyceraldehyde-3-phospate dehydrogenase from M. tuberculosis in E. coli cells in soluble and catalytically active form and to elaborate a method for the purification of the recombinant enzyme. The...
The effects of overexpression of cytoplasmic chaperones on secretory production of hirudin-PA in E. coli [0.03%]
胞质分子伴侣过表达对大肠杆菌中hirudin-PA分泌型生产的影响研究
Nafiseh Alsadat Seyed Hosseini Fin,Mohammad Barshan-Tashnizi,Seyed Mehdi Sajjadi et al.
Nafiseh Alsadat Seyed Hosseini Fin et al.
The secretory production of heterologous proteins in E. coli has revolutionized biotechnology. Efficient periplasmic production of foreign proteins in E. coli often requires a signal peptide to direct proteins to the periplasm. However, the...
Review of lactose-driven auto-induction expression of isotope-labelled proteins [0.03%]
基于乳糖的诱导表达同位素标记蛋白研究进展
Erika L Crowley,Steven P Rafferty
Erika L Crowley
NMR is an important method in the structural and functional characterization of proteins, but such experiments typically require isotopic labelling because of the low natural abundance of the nuclei of interest. Isotope-labelled protein for...
The catalytic domain of cathepsin C (dipeptidyl-peptidase I) alone is a fully functional endoprotease [0.03%]
单独的猫肉蛋白C（二肽基肽酶I）催化结构域是一个完全具有功能的内肽酶
Mateja Rebernik,Brigita Lenarčič,Marko Novinec
Mateja Rebernik
Cathepsin C is a tetrameric lysosomal protease that acts as a dipeptidyl-peptidase due to the presence of the exclusion domain that is unique among papain-like cysteine proteases. Here we describe a recombinant form of cathepsin C lacking i...
One-step production of bioactive human lipopolysaccharide binding protein from LPS-eliminated E. coli [0.03%]
一步生产无LPS活性人的脂多糖结合蛋白
Jie Qiao,Ce Dong,Xinping Wang et al.
Jie Qiao et al.
Human lipopolysaccharide (LPS) binding protein (LBP) is a ∼60 kDa glycosylated protein that mediates potent innate immune against invading Gram-negative bacteria by recognition of LPS in their outer membranes. To date, there is no method f...
Chaperone-assisted expression and purification of putrescine monooxygenase from Shewanella putrefaciens-95 [0.03%]
恶臭假单胞菌脯氨单氧化酶的伴侣辅助表达及纯化
Narsing Rao Saroja,Anil H Shyam Mohan,D Srividya et al.
Narsing Rao Saroja et al.
A putrescine monooxygenase from Shewanella putrefaciens 95 (SpPMO) is the initial enzyme catalyzing the hydroxylation of putrescine to N-hydroxyl putrescine, the precursor for the synthesis of a siderophore putrebactin was identified. This ...
Expression and biochemical characterization of a new alkaline tannase from Lactobacillus pentosus [0.03%]
一株植物乳杆菌来源的新型碱性单宁酶的表达及生化性质研究
Apinun Kanpiengjai,Kridsada Unban,Thu-Ha Nguyen et al.
Apinun Kanpiengjai et al.
Lactobacillus pentosus BA-7 and L. pentosus QA1-5 are tannin-tolerant lactic acid bacteria that were isolated from Miang, a traditional fermented tea-leaf found in northern Thailand and a tannin-rich substrate. Tannase encoding genes were i...