Mobile genetic elements beyond the VanB-resistance dissemination among hospital-associated enterococci and other Gram-positive bacteria [0.03%]
医院相关肠球菌及其他革兰氏阳性菌中VanB耐药性传播的移动遗传元件之外的因素
Ewa Sadowy
Ewa Sadowy
An increasing resistance to vancomycin among clinically relevant enterococci, such as Enterococcus faecalis and Enterococcus faecium is a cause of a great concern, as it seriously limits treatment options. The vanB operon is one of most com...
Characterization of blaCMY-2-carrying IncC and rmtB-carrying IncI1/ST136 plasmids in an avian Escherichia coli ST224 strain [0.03%]
分离于禽源大肠埃希菌ST224株的携带blaCMY-2基因的IncC质粒和携带rmtB基因的IncI1/ST136型耐药质粒的特征分析
Dan-Dan He,Meng-Mei Cui,Teng-Li Zhang et al.
Dan-Dan He et al.
To analyze characteristics and underlying evolutionary processes of IncC and IncI1 plasmids in a multidrug-resistant avian E. coli strain, antibiotic susceptibility testing, PCR, conjugation assays, and next-generation sequencing were perfo...
Enhancement of transgene expression by the β-catenin inhibitor iCRT14 [0.03%]
β连环蛋白抑制剂iCRT14可增强转基因表达
Kyle Spivack,Christine Muzzelo,Matthew Hall et al.
Kyle Spivack et al.
The innate immune response is an essential defense mechanism that allows cells to detect pathogen-associated molecular patterns (PAMPs) like endotoxin or cytosolic DNA and then induce the expression of defensive genes that restrict the repl...
Improved pEKEx2-derived expression vectors for tightly controlled production of recombinant proteins in Corynebacterium glutamicum [0.03%]
用于谷氨酸棒杆菌中重组蛋白的严格控制生产的改进型pEKEx2衍生表达载体
Patrick J Bakkes,Paul Ramp,Astrid Bida et al.
Patrick J Bakkes et al.
The Escherichia coli/Corynebacterium glutamicum shuttle vector pEKEx2 is an IPTG-inducible expression vector that has been used successfully for the synthesis of numerous proteins in C. glutamicum. We discovered that the leaky gene expressi...
Evolution of IS26-bounded pseudo-compound transposons carrying the tet(C) tetracycline resistance determinant [0.03%]
携带四环素抗性决定子tet(C)的IS26界定了伪复合转座子的进化过程
Carol H Pong,Robert A Moran,Ruth M Hall
Carol H Pong
A large plasmid, pCERC14, found in an antibiotic resistant commensal Escherichia coli isolate recovered from a healthy adult was sequenced. pCERC14 was 162,926 bp and carried FII-18 and FIB-1 replicons and an F-like transfer region as well ...
Novel conditional plasmids regulated by chemical switches provide versatile tools for genetic engineering in Escherichia coli [0.03%]
新型化学开关调控的条件性质粒为大肠杆菌基因工程提供了多功能工具
André Riedl,Simone Gruber,Zsolt Ruzsics
André Riedl
Engineering bacterial genomes or foreign DNA cloned as bacterial artificial chromosomes (BACs) relies on usage of helper plasmids, which deliver the desired tools transiently into the bacteria to be modified. After the anticipated action is...
Structures bounded by directly-oriented members of the IS26 family are pseudo-compound transposons [0.03%]
由IS26家族直接定向的成员限定的结构是假复合转座子
Christopher J Harmer,Carol H Pong,Ruth M Hall
Christopher J Harmer
Antibiotic resistance genes are often found in structures bounded by copies of IS26, IS257/IS431 or IS1216 that resemble compound (or composite) transposons. However, because of the mechanisms used by IS26 family members, namely that they f...
Antimicrobial resistance gene shuffling and a three-element mobilisation system in the monophasic Salmonella typhimurium strain ST1030 [0.03%]
单相鼠伤寒沙门氏菌ST1030株中抗微生物耐药基因的重排和三元件动员系统
M Oliva,C Calia,M Ferrara et al.
M Oliva et al.
In this study we describe the genetic elements and the antimicrobial resistance units (RUs) harboured by the Salmonella Typhimurium monophasic variant 1,4,[5],12:i:- strain ST1030. Of the three identified RUs two were chromosomal, RU1 (IS26...
Evolution and dissemination of L and M plasmid lineages carrying antibiotic resistance genes in diverse Gram-negative bacteria [0.03%]
携带抗性基因的L和M质粒在不同革兰氏阴性菌中的起源与传播
Grace A Blackwell,Emma L Doughty,Robert A Moran
Grace A Blackwell
Conjugative, broad host-range plasmids of the L/M complex have been associated with antibiotic resistance since the 1970s. They are found in Gram-negative bacterial genera that cause human infections and persist in hospital environments. It...
Incorporating the plasmidome into antibiotic resistance surveillance in animal agriculture [0.03%]
将质粒组学纳入到动物农业抗生素耐药性监测中
N Ricker,B S Spoja,N May et al.
N Ricker et al.
Mobile genetic elements (MGE) carrying resistance genes represent a unique challenge to risk assessment and surveillance of antimicrobial resistance (AMR). Yet determining the mobility of resistance genes within animal microbiomes is essent...