Insights into the individual evolutionary origins of Yersinia virulence factor effector proteins [0.03%]
耶氏菌效应蛋白毒力因子的个体进化起源研究新见解
Veronica R Moorman,James I Cohen
Veronica R Moorman
Pathogenic Yersinia bacteria, including Y. pseudotubuclosis Y. enterocolitica, and Y. pestis, contain the mosaic plasmid pYV that encodes for, among other things, a number of proteinaceous virulence factors. While the evolutionary histories...
An X1α plasmid from a Salmonella enterica serovar Ohio isolate carrying a novel IS26-bounded tet(C) pseudo-compound transposon [0.03%]
来自伤寒沙门菌俄亥俄血清型分离株的含有新的IS26边界tet(C)假复合转座子的X1α质粒
Carol H Pong,Ruth M Hall
Carol H Pong
The sequence of a conjugative plasmid, pSRC22-2, found in a multiply antibiotic resistant Salmonella enterica serovar Ohio isolate SRC22 originally cultured from swine in 1999, was determined. Plasmid pSRC22-2 has a copy number of approxima...
Transcriptomic analysis of Entamoeba histolytica reveals domain-specific sense strand expression of LINE-encoded ORFs with massive antisense expression of RT domain [0.03%]
衣氏肺孢子虫转录组分析揭示LINE-编码ORF的有义链表达具有领域特异性，而逆转录酶结构域则大量表达反义链
Devinder Kaur,Mridula Agrahari,Shashi Shekhar Singh et al.
Devinder Kaur et al.
LINEs are retrotransposable elements found in diverse organisms. Their activity is kept in check by several mechanisms, including transcriptional silencing. Here we have analyzed the transcription status of LINE1 copies in the early-branchi...
Functional analysis of the catalytic triad of the hAT-family transposase TcBuster [0.03%]
hAT家族转位酶TcBuster催化三联体的功能分析
Lauren E Woodard,Felisha M Williams,Isria C Jarrett et al.
Lauren E Woodard et al.
TcBuster is a hAT-family DNA transposon from the red flour beetle, Tribolium castaneum. The TcBuster transposase is of interest for genome engineering as it is highly active in insect and mammalian cells. To test the predicted catalytic tri...
Plasmid localization of sole rrn operon in genomes of Oecophyllibacter saccharovorans (Acetobacteraceae) [0.03%]
Oecophylla bacterium 基因组中唯一的rrn操纵子定位于质粒上
Kah-Ooi Chua,Wah-Seng See-Too,Hoi-Sen Yong et al.
Kah-Ooi Chua et al.
The bacterium Oecophyllibacter saccharovorans of family Acetobacteraceae is a symbiont of weaver ant Oecophylla smaragdina. In our previous study, we published the finding of novel O. saccharovorans strains Ha5T, Ta1 and Jb2 (Chua et al. 20...
Mobile genetic elements beyond the VanB-resistance dissemination among hospital-associated enterococci and other Gram-positive bacteria [0.03%]
医院相关肠球菌及其他革兰氏阳性菌中VanB耐药性传播的移动遗传元件之外的因素
Ewa Sadowy
Ewa Sadowy
An increasing resistance to vancomycin among clinically relevant enterococci, such as Enterococcus faecalis and Enterococcus faecium is a cause of a great concern, as it seriously limits treatment options. The vanB operon is one of most com...
Characterization of blaCMY-2-carrying IncC and rmtB-carrying IncI1/ST136 plasmids in an avian Escherichia coli ST224 strain [0.03%]
分离于禽源大肠埃希菌ST224株的携带blaCMY-2基因的IncC质粒和携带rmtB基因的IncI1/ST136型耐药质粒的特征分析
Dan-Dan He,Meng-Mei Cui,Teng-Li Zhang et al.
Dan-Dan He et al.
To analyze characteristics and underlying evolutionary processes of IncC and IncI1 plasmids in a multidrug-resistant avian E. coli strain, antibiotic susceptibility testing, PCR, conjugation assays, and next-generation sequencing were perfo...
Enhancement of transgene expression by the β-catenin inhibitor iCRT14 [0.03%]
β连环蛋白抑制剂iCRT14可增强转基因表达
Kyle Spivack,Christine Muzzelo,Matthew Hall et al.
Kyle Spivack et al.
The innate immune response is an essential defense mechanism that allows cells to detect pathogen-associated molecular patterns (PAMPs) like endotoxin or cytosolic DNA and then induce the expression of defensive genes that restrict the repl...
Improved pEKEx2-derived expression vectors for tightly controlled production of recombinant proteins in Corynebacterium glutamicum [0.03%]
用于谷氨酸棒杆菌中重组蛋白的严格控制生产的改进型pEKEx2衍生表达载体
Patrick J Bakkes,Paul Ramp,Astrid Bida et al.
Patrick J Bakkes et al.
The Escherichia coli/Corynebacterium glutamicum shuttle vector pEKEx2 is an IPTG-inducible expression vector that has been used successfully for the synthesis of numerous proteins in C. glutamicum. We discovered that the leaky gene expressi...
Evolution of IS26-bounded pseudo-compound transposons carrying the tet(C) tetracycline resistance determinant [0.03%]
携带四环素抗性决定子tet(C)的IS26界定了伪复合转座子的进化过程
Carol H Pong,Robert A Moran,Ruth M Hall
Carol H Pong
A large plasmid, pCERC14, found in an antibiotic resistant commensal Escherichia coli isolate recovered from a healthy adult was sequenced. pCERC14 was 162,926 bp and carried FII-18 and FIB-1 replicons and an F-like transfer region as well ...