One-step construction of a food-grade expression system based on the URA3 gene in Kluyveromyces lactis [0.03%]
基于URA3基因的一步构建乳酒球酵母食品级表达系统的方法研究
Zhicheng Liang,Mulan Deng,Zhi Zhang et al.
Zhicheng Liang et al.
Proteins from food-grade expression systems can be used in food products and medical applications. Herein, we describe a one-step method of constructing an expression vector in Kluyveromyces lactis by combining a URA3-deficient strain and a...
Interference of ISEcp1-blaCTX-M-1 with the shufflon rearrangement in IncI1 plasmids [0.03%]
ISEcp1-blaCTX-M-1对IncI1质粒shufflon重组的干扰作用
Milan S Stosic,Marianne Sunde,Solveig Sølverød Mo et al.
Milan S Stosic et al.
IncI1 plasmids are known disseminators of the extended-spectrum cephalosporin resistance (ESC) gene blaCTX-M-1, among species of the Enterobacteriaceae family. In several IncI1 plasmids, this gene was found incorporated into the transpositi...
An improved direct metamobilome approach increases the detection of larger-sized circular elements across kingdoms [0.03%]
改进的直接代谢组方法增加了跨越各大类群的大型共价封闭元件的检测数量
Katrine Wacenius Skov Alanin,Tue Sparholt Jørgensen,Patrick Denis Browne et al.
Katrine Wacenius Skov Alanin et al.
Mobile genetic elements (MGEs) are instrumental in natural prokaryotic genome editing, permitting genome plasticity and allowing microbes to accumulate genetic diversity. MGEs serve as a vast communal gene pool and include DNA elements such...
Molecular characterization of two novel NDM-1-producing atypical enteroaggregative Escherichia coli isolates from patients [0.03%]
来自两株新型产NDM-1泛志贺毒素的大肠埃希菌的分子特征研究
Pengcheng Du,Pei Zhang,Juan Wang et al.
Pengcheng Du et al.
To investigate NDM-1-producing atypical Enteroaggregative Escherichia coli (aEAEC) of sequence type 349 from hospitalized patients, the isolates 13ZX28 and 13ZX36 were subjected to antimicrobial susceptibility testing, conjugation and whole...
The extrachromosomal elements of the Naegleria genus: How little we know [0.03%]
汉氏阿米巴属的细胞外染色体元件：我们知之甚少的事实
B T Nguyen,N M Chapman,S Tracy et al.
B T Nguyen et al.
There are currently 47 characterized species in the Naegleria genus of free-living amoebae. Each amoeba has thousands of extrachromosomal elements that are closed circular structures comprised of a single ribosomal DNA (rDNA) copy and a lar...
Utilization of a tetracycline-inducible system for high-level expression of recombinant proteins in Francisella tularensis LVS [0.03%]
四环素诱导系统在LVS中的应用及重组蛋白的高效表达
Valeria Sheshko,Marek Link,Igor Golovliov et al.
Valeria Sheshko et al.
Francisella tularensis is a Gram-negative intracellular pathogen causing tularemia. A number of its potential virulence factors have been identified, but their biology and functions are not precisely known. Understanding the biological and ...
Genomic islands related to Salmonella genomic island 1; integrative mobilisable elements in trmE mobilised in trans by A/C plasmids [0.03%]
与沙门氏菌基因组岛1相关的基因组岛屿；由A/C质粒通过trmE基因座转座的整合可移动元件
Claire de Curraize,Eliane Siebor,Catherine Neuwirth
Claire de Curraize
Salmonella genomic island 1 (SGI1), an integrative mobilisable element (IME), was first reported 20 years ago, in the multidrug resistant Salmonella Typhimurium DT104 clone. Since this first report, many variants and relatives have been fou...
Identification and characterization of a spreadable IncI1 plasmid harbouring a blaCTX-M-15 gene in an Italian human isolate of Salmonella serovar Napoli [0.03%]
意大利沙门氏菌奈亚比型菌株中携带blaCTX-M-15基因的可移动IncI1质粒的鉴定和表征
Sara Petrin,Massimiliano Orsini,Eleonora Mastrorilli et al.
Sara Petrin et al.
Salmonella enterica subsp. enterica serovar Napoli (S. Napoli) ranks among the top serovars causing human infections in Italy, although not common in other European countries. Isolates are generally pan-susceptible or resistant to aminoglyc...
Reciprocal relation between reporter gene transcription and translation efficiency in fission yeast [0.03%]
裂殖酵母报道基因转录与翻译效率间的互惠关系研究
Suchita Srivastava,Satinderdeep Kaur,Hemant K Verma et al.
Suchita Srivastava et al.
The fission yeast, Schizosaccharomyces pombe, is an excellent model for basic research but is not useful for commercial scale protein expression due to lack of strong expression vectors. Earlier, we showed that the lsd90 promoter elicited s...
Novel class 1 integron harboring antibiotic resistance genes in wastewater-derived bacteria as revealed by functional metagenomics [0.03%]
基于功能元基因组学的废水菌群中新型I类整合子及耐药基因的研究
Bridget B McGivern,Rylie K McDonell,Sydney K Morris et al.
Bridget B McGivern et al.
Combatting antibiotic resistance is critical to our ability to treat infectious diseases. Here, we identified and characterized diverse antimicrobial resistance genes, including potentially mobile elements, from synthetic wastewater treatme...