Aaron Nicholas Gillman,Alexandra Helleux,Sören Abel
Aaron Nicholas Gillman
We developed a simplified, highly efficient Gateway reaction that recombines target DNA to expression (destination) plasmids in vivo and subsequently conjugates the final vector into a recipient strain, all in a single step. This recipient ...
Frequency and diversity of small plasmids in mesophilic Aeromonas isolates from fish, water and sediment [0.03%]
中温性Aeromonas菌株中小质粒的频率和多样性，这些菌株来自鱼、水和沉积物
Daniel Pérez-García,Violeta Larios-Serrato,Rogelio Rojas-Rios et al.
Daniel Pérez-García et al.
Plasmids are autonomous genetic elements ubiquitously present in bacteria. In addition to containing genetic determinants responsible for their replication and stability, some plasmids may carry genes that help bacteria adapt to different e...
Sequence polarity between the promoter and the adjacent gene modulates promoter activity [0.03%]
启动子与其相邻基因之间的序列极性调控启动子活性
Tam T Tran,Trevor C Charles
Tam T Tran
Promoter engineering has been employed as a strategy to enhance and optimize the production of bio-products. Availability of promoters with predictable activities is needed for downstream application. However, whether promoter activity rema...
An expression system of channelrhodopsin-2 driven by a minimal Arc/Arg3.1 promoter and Tet system was developed in human neuroblastoma cells [0.03%]
在人类神经母细胞瘤细胞中开发了由最小的Arc/Arg3.1启动子和Tet系统驱动的Channelrhodopsin-2表达系统
Akara Metasuk,Narisorn Kitiyanant,Banthit Chetsawang
Akara Metasuk
Advances in neuroscience have relied on the development of techniques that examine neuronal cell activities. One major challenge involves the limitations in labeling and controlling neuronal activities relating to the cell's activation stat...
Li Liu,Yue Chen,Shiqin Yu et al.
Li Liu et al.
Gluconobacter oxydans is an obligate Gram-negative bacterium that belongs to the family Acetobacteraceae. It is one of the most frequently used microorganisms in industrial biotechnology to produce chemicals related to incomplete oxidation....
One-step construction of a food-grade expression system based on the URA3 gene in Kluyveromyces lactis [0.03%]
基于URA3基因的一步构建乳酒球酵母食品级表达系统的方法研究
Zhicheng Liang,Mulan Deng,Zhi Zhang et al.
Zhicheng Liang et al.
Proteins from food-grade expression systems can be used in food products and medical applications. Herein, we describe a one-step method of constructing an expression vector in Kluyveromyces lactis by combining a URA3-deficient strain and a...
Interference of ISEcp1-blaCTX-M-1 with the shufflon rearrangement in IncI1 plasmids [0.03%]
ISEcp1-blaCTX-M-1对IncI1质粒shufflon重组的干扰作用
Milan S Stosic,Marianne Sunde,Solveig Sølverød Mo et al.
Milan S Stosic et al.
IncI1 plasmids are known disseminators of the extended-spectrum cephalosporin resistance (ESC) gene blaCTX-M-1, among species of the Enterobacteriaceae family. In several IncI1 plasmids, this gene was found incorporated into the transpositi...
An improved direct metamobilome approach increases the detection of larger-sized circular elements across kingdoms [0.03%]
改进的直接代谢组方法增加了跨越各大类群的大型共价封闭元件的检测数量
Katrine Wacenius Skov Alanin,Tue Sparholt Jørgensen,Patrick Denis Browne et al.
Katrine Wacenius Skov Alanin et al.
Mobile genetic elements (MGEs) are instrumental in natural prokaryotic genome editing, permitting genome plasticity and allowing microbes to accumulate genetic diversity. MGEs serve as a vast communal gene pool and include DNA elements such...
Molecular characterization of two novel NDM-1-producing atypical enteroaggregative Escherichia coli isolates from patients [0.03%]
来自两株新型产NDM-1泛志贺毒素的大肠埃希菌的分子特征研究
Pengcheng Du,Pei Zhang,Juan Wang et al.
Pengcheng Du et al.
To investigate NDM-1-producing atypical Enteroaggregative Escherichia coli (aEAEC) of sequence type 349 from hospitalized patients, the isolates 13ZX28 and 13ZX36 were subjected to antimicrobial susceptibility testing, conjugation and whole...
The extrachromosomal elements of the Naegleria genus: How little we know [0.03%]
汉氏阿米巴属的细胞外染色体元件：我们知之甚少的事实
B T Nguyen,N M Chapman,S Tracy et al.
B T Nguyen et al.
There are currently 47 characterized species in the Naegleria genus of free-living amoebae. Each amoeba has thousands of extrachromosomal elements that are closed circular structures comprised of a single ribosomal DNA (rDNA) copy and a lar...