Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures [0.03%]
CHO-K1细胞内源性启动子筛选及其在哺乳动物细胞培养中表达重组蛋白的功能分析
Ileana Tossolini,Agustina Gugliotta,Fernando López Díaz et al.
Ileana Tossolini et al.
For the production of recombinant protein therapeutics in mammalian cells, a high rate of gene expression is desired and hence strong viral-derived promoters are commonly used. However, they usually induce cellular stress and can be suscept...
The fluorination effect on the transfection efficacy of cell penetrating peptide complexes [0.03%]
穿膜肽复合物的氟化效应对其转染效率的影响研究
Abdulgader A Baoum
Abdulgader A Baoum
Cell penetrating peptides (CPPs) have been used as alternative delivery vectors to translocate therapeutic cargo molecules across cell membranes. One example of CPPs is the dTAT peptide, which has shown great promise in the design of highly...
Complete sequence of classic F-type plasmid pRK100 shows unique conservation over time and geographic location [0.03%]
经典F型质粒pRK100的完整序列在时间和地理分布上显示出独特的保守性
Marjanca Starčič Erjavec,Karmen Jeseničnik,Lauren P Elam et al.
Marjanca Starčič Erjavec et al.
Plasmids exhibit great diversity of gene content and host ranges and are famous for quick adaptation to the genetic background of the bacterial host cell. In addition to observing ever evolving plasmids, some plasmids have conserved backbon...
A role for the last C-terminal helix of the F plasmid segregating protein SopA in nucleoid binding and plasmid maintenance [0.03%]
F因子分配蛋白SopA的C端末端螺旋在染色体结合和质粒维持中的作用
Dipika Mishra,Anirudh Jakhmola,Ramanujam Srinivasan
Dipika Mishra
The rapid emergence and spread of antibiotic resistance is a growing global burden. Antibiotic resistance is often associated with large single or low copy number plasmids, which rely upon cytoskeletal proteins for their stable maintenance....
Replication initiator proteins of Acinetobacter baumannii plasmids: An update note [0.03%]
阿 klebsiella 质粒复制起始蛋白：最新研究报告
Semiramis Castro-Jaimes,Gabriela Guerrero,Elena Bello-López et al.
Semiramis Castro-Jaimes et al.
The bioinformatic analysis that we made of 492 Acinetobacter baumannii plasmid sequences identified 418 genes encoding Replication Initiator (Rep) proteins that fell into at least fourteen groups according to the protein domains that they c...
Aaron Nicholas Gillman,Alexandra Helleux,Sören Abel
Aaron Nicholas Gillman
We developed a simplified, highly efficient Gateway reaction that recombines target DNA to expression (destination) plasmids in vivo and subsequently conjugates the final vector into a recipient strain, all in a single step. This recipient ...
Frequency and diversity of small plasmids in mesophilic Aeromonas isolates from fish, water and sediment [0.03%]
中温性Aeromonas菌株中小质粒的频率和多样性，这些菌株来自鱼、水和沉积物
Daniel Pérez-García,Violeta Larios-Serrato,Rogelio Rojas-Rios et al.
Daniel Pérez-García et al.
Plasmids are autonomous genetic elements ubiquitously present in bacteria. In addition to containing genetic determinants responsible for their replication and stability, some plasmids may carry genes that help bacteria adapt to different e...
Sequence polarity between the promoter and the adjacent gene modulates promoter activity [0.03%]
启动子与其相邻基因之间的序列极性调控启动子活性
Tam T Tran,Trevor C Charles
Tam T Tran
Promoter engineering has been employed as a strategy to enhance and optimize the production of bio-products. Availability of promoters with predictable activities is needed for downstream application. However, whether promoter activity rema...
An expression system of channelrhodopsin-2 driven by a minimal Arc/Arg3.1 promoter and Tet system was developed in human neuroblastoma cells [0.03%]
在人类神经母细胞瘤细胞中开发了由最小的Arc/Arg3.1启动子和Tet系统驱动的Channelrhodopsin-2表达系统
Akara Metasuk,Narisorn Kitiyanant,Banthit Chetsawang
Akara Metasuk
Advances in neuroscience have relied on the development of techniques that examine neuronal cell activities. One major challenge involves the limitations in labeling and controlling neuronal activities relating to the cell's activation stat...
Li Liu,Yue Chen,Shiqin Yu et al.
Li Liu et al.
Gluconobacter oxydans is an obligate Gram-negative bacterium that belongs to the family Acetobacteraceae. It is one of the most frequently used microorganisms in industrial biotechnology to produce chemicals related to incomplete oxidation....