Plasmidome analysis of a hospital effluent biofilm: Status of antibiotic resistance [0.03%]
医院废水生物膜的质粒组分析：抗生素抗性的状况
Claire Hennequin,Christiane Forestier,Ousmane Traore et al.
Claire Hennequin et al.
Plasmids are widely involved in the dissemination of characteristics within bacterial communities. Their genomic content can be assessed by high-throughput sequencing of the whole plasmid fraction of an environment, the plasmidome. In this ...
Brick into the Gateway (BiG): A novel approach for faster cloning combining Golden Gate and Gateway methods [0.03%]
砖块进入门式法（BIG）：结合Golden Gate和Gateway方法的快速克隆新策略
Letícia F Ferigolo,Mateus H Vicente,Fabio T S Nogueira
Letícia F Ferigolo
Gateway system is one of the most known cloning systems, which makes it compatible with several expression vectors, including those used for Yeast Two-Hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assays. However, this sy...
Characterization of the Agrobacterium octopine-cucumopine catabolic plasmid pAtAg67 [0.03%]
阿黑菌八氢番茄红素/六氢番茄红素降解性质粒pAtAg67的表征
Marjolein J G Hooykaas,Shuai Shao,Paul J J Hooykaas
Marjolein J G Hooykaas
In addition to tumor-inducing agrobacteria, non-pathogenic strains are often isolated from crown gall tumors. Such non-pathogenic strains sometimes contain catabolic plasmids that allow them to take advantage of the opines produced in the t...
Evolution of Acinetobacter baumannii plasmids carrying the oxa58 carbapenemase resistance gene via plasmid fusion, IS26-mediated events and dif module shuffling [0.03%]
耐碳青霉烯类肺炎克雷伯菌中携带不同bla基因的质粒结构及转移机制研究
Naomi I Jones,Christopher J Harmer,Mohammad Hamidian et al.
Naomi I Jones et al.
Acinetobacter baumannii RepAci1-RepAci10 plasmids pA388 from a global clone 1 (GC1) isolate from Greece, and pACICU1 and variant pACICU1b from an Italian GC2 isolate were found to share a common ancestor. The ancestor formed via recombinati...
Estimating plasmid conjugation rates: A new computational tool and a critical comparison of methods [0.03%]
估算质粒接合率：一个新的计算工具和方法批判性比较
Jana S Huisman,Fabienne Benz,Sarah J N Duxbury et al.
Jana S Huisman et al.
Plasmids are important vectors for the spread of genes among diverse populations of bacteria. However, there is no standard method to determine the rate at which they spread horizontally via conjugation. Here, we compare commonly used metho...
Screening of CHO-K1 endogenous promoters for expressing recombinant proteins in mammalian cell cultures [0.03%]
CHO-K1细胞内源性启动子筛选及其在哺乳动物细胞培养中表达重组蛋白的功能分析
Ileana Tossolini,Agustina Gugliotta,Fernando López Díaz et al.
Ileana Tossolini et al.
For the production of recombinant protein therapeutics in mammalian cells, a high rate of gene expression is desired and hence strong viral-derived promoters are commonly used. However, they usually induce cellular stress and can be suscept...
The fluorination effect on the transfection efficacy of cell penetrating peptide complexes [0.03%]
穿膜肽复合物的氟化效应对其转染效率的影响研究
Abdulgader A Baoum
Abdulgader A Baoum
Cell penetrating peptides (CPPs) have been used as alternative delivery vectors to translocate therapeutic cargo molecules across cell membranes. One example of CPPs is the dTAT peptide, which has shown great promise in the design of highly...
Complete sequence of classic F-type plasmid pRK100 shows unique conservation over time and geographic location [0.03%]
经典F型质粒pRK100的完整序列在时间和地理分布上显示出独特的保守性
Marjanca Starčič Erjavec,Karmen Jeseničnik,Lauren P Elam et al.
Marjanca Starčič Erjavec et al.
Plasmids exhibit great diversity of gene content and host ranges and are famous for quick adaptation to the genetic background of the bacterial host cell. In addition to observing ever evolving plasmids, some plasmids have conserved backbon...
A role for the last C-terminal helix of the F plasmid segregating protein SopA in nucleoid binding and plasmid maintenance [0.03%]
F因子分配蛋白SopA的C端末端螺旋在染色体结合和质粒维持中的作用
Dipika Mishra,Anirudh Jakhmola,Ramanujam Srinivasan
Dipika Mishra
The rapid emergence and spread of antibiotic resistance is a growing global burden. Antibiotic resistance is often associated with large single or low copy number plasmids, which rely upon cytoskeletal proteins for their stable maintenance....
Replication initiator proteins of Acinetobacter baumannii plasmids: An update note [0.03%]
阿 klebsiella 质粒复制起始蛋白：最新研究报告
Semiramis Castro-Jaimes,Gabriela Guerrero,Elena Bello-López et al.
Semiramis Castro-Jaimes et al.
The bioinformatic analysis that we made of 492 Acinetobacter baumannii plasmid sequences identified 418 genes encoding Replication Initiator (Rep) proteins that fell into at least fourteen groups according to the protein domains that they c...