Genomic analysis of plasmid content in food isolates of E. coli strongly supports its role as a reservoir for the horizontal transfer of virulence and antibiotic resistance genes [0.03%]
基因组分析表明食物来源的大肠杆菌质粒含量丰富，是耐药和致病基因水平转移的重要汇合地
María G Balbuena-Alonso,Gerardo Cortés-Cortés,Jay W Kim et al.
María G Balbuena-Alonso et al.
The link between E. coli strains contaminating foods and human disease is unclear, with some reports supporting a direct transmission of pathogenic strains via food and others highlighting their role as reservoirs for resistance and virulen...
An easily modifiable conjugative plasmid for studying horizontal gene transfer [0.03%]
一种可轻松修改的接合质粒用于研究水平基因转移
Qinqin Wang,Asmus Kalckar Olesen,Lorrie Maccario et al.
Qinqin Wang et al.
Horizontal gene transfer is an important mechanism in bacterial evolution and can occur at striking frequencies when mediated by mobile genetic elements. Conjugative plasmids are mobile genetic elements that are main drivers of horizontal t...
Implementation of a tunable t-CRISPRi system for gene regulation in Giardia duodenalis [0.03%]
在十二指肠毛滴虫中实现可调t-CRISPRi系统以调节基因表达
Eduardo García-Huerta,Sara Espinoza-Corona,Francisco Alejandro Lagunas-Rangel et al.
Eduardo García-Huerta et al.
Giardia duodenalis, is a binuclear and microaerophilic protozoan that causes giardiasis. Up to date, several molecular approaches have been taken to understand the molecular mechanisms of diverse cellular processes in this parasitic protozo...
Genetic editing of multi-resistance plasmids in Escherichia coli isolated from meat during transfer [0.03%]
来自肉品的多重耐药质粒型大肠埃希氏菌在传递过程中的基因编辑特性研究
Tania S Darphorn,Stanley Brul,Benno H Ter Kuile
Tania S Darphorn
Resistance plasmids mediate the rapid spread of antimicrobial resistance, which poses a threat to veterinary and human healthcare. This study addresses the question whether resistance plasmids from Escherichia coli isolated from foodstuffs ...
Conjugation-mediated transfer of pXO16, a large plasmid from Bacillus thuringiensis sv. israelensis, across the Bacillus cereus group and its impact on host phenotype [0.03%]
Bacillus.thuringiensis.israelensis来源的大质粒pXO16的接合转移及其对宿主表型的影响研究
Pauline Hinnekens,Jacques Mahillon
Pauline Hinnekens
pXO16, the 350 kb-conjugative plasmid from Bacillus thuringiensis sv. israelensis promotes its own transfer at high efficiency, triggers the transfer of mobilizable and non-mobilizable plasmids, as well as the transfer of host chromosomal l...
Plasmidome analysis of a hospital effluent biofilm: Status of antibiotic resistance [0.03%]
医院废水生物膜的质粒组分析：抗生素抗性的状况
Claire Hennequin,Christiane Forestier,Ousmane Traore et al.
Claire Hennequin et al.
Plasmids are widely involved in the dissemination of characteristics within bacterial communities. Their genomic content can be assessed by high-throughput sequencing of the whole plasmid fraction of an environment, the plasmidome. In this ...
Brick into the Gateway (BiG): A novel approach for faster cloning combining Golden Gate and Gateway methods [0.03%]
砖块进入门式法（BIG）：结合Golden Gate和Gateway方法的快速克隆新策略
Letícia F Ferigolo,Mateus H Vicente,Fabio T S Nogueira
Letícia F Ferigolo
Gateway system is one of the most known cloning systems, which makes it compatible with several expression vectors, including those used for Yeast Two-Hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assays. However, this sy...
Characterization of the Agrobacterium octopine-cucumopine catabolic plasmid pAtAg67 [0.03%]
阿黑菌八氢番茄红素/六氢番茄红素降解性质粒pAtAg67的表征
Marjolein J G Hooykaas,Shuai Shao,Paul J J Hooykaas
Marjolein J G Hooykaas
In addition to tumor-inducing agrobacteria, non-pathogenic strains are often isolated from crown gall tumors. Such non-pathogenic strains sometimes contain catabolic plasmids that allow them to take advantage of the opines produced in the t...
Evolution of Acinetobacter baumannii plasmids carrying the oxa58 carbapenemase resistance gene via plasmid fusion, IS26-mediated events and dif module shuffling [0.03%]
耐碳青霉烯类肺炎克雷伯菌中携带不同bla基因的质粒结构及转移机制研究
Naomi I Jones,Christopher J Harmer,Mohammad Hamidian et al.
Naomi I Jones et al.
Acinetobacter baumannii RepAci1-RepAci10 plasmids pA388 from a global clone 1 (GC1) isolate from Greece, and pACICU1 and variant pACICU1b from an Italian GC2 isolate were found to share a common ancestor. The ancestor formed via recombinati...
Estimating plasmid conjugation rates: A new computational tool and a critical comparison of methods [0.03%]
估算质粒接合率：一个新的计算工具和方法批判性比较
Jana S Huisman,Fabienne Benz,Sarah J N Duxbury et al.
Jana S Huisman et al.
Plasmids are important vectors for the spread of genes among diverse populations of bacteria. However, there is no standard method to determine the rate at which they spread horizontally via conjugation. Here, we compare commonly used metho...