Mikkel M Liljegren,João A Gama,Pål J Johnsen et al.
Mikkel M Liljegren et al.
Plasmids can impact the evolution of their hosts, e.g. due to carriage of mutagenic genes, through cross-talk with host genes or as result of SOS induction during transfer. Here we demonstrate that plasmids can affect the level of microinde...
Characterization and functional insights of the novel RC-type plasmid pAnox1 from Anoxybacillus gonensis 05S15 [0.03%]
来自芽单胞菌属厌氧谷氏细菌05S15的新型RC型质粒pAnox1的表征及其功能研究
Gamze Cubukci,Hatice Ayyildiz,Kadriye Inan Bektas et al.
Gamze Cubukci et al.
The plasmid pAnox1, isolated from Anoxybacillus gonensis 05S15, was sequenced and characterized as a circular, double-stranded DNA molecule of 1592 base pairs with a GC content of 40.01 %. Despite its cryptic nature and small genome, bioinf...
miRNA heterologous production in bacteria: A systematic review focusing on the choice of plasmid features and bacterial/prokaryotic microfactory [0.03%]
基于细菌的外源性microRNA异源生产：以质粒特性选择及细菌/原核生物微流控反应器为重点的系统综述
Nyelson da Silva Nonato,Leandro Silva Nunes,Amanda Weege da Silveira Martins et al.
Nyelson da Silva Nonato et al.
Bacteria, the primary microorganisms used for industrial molecule production, do not naturally generate miRNAs. This study aims to systematically review current literature on miRNA expression systems in bacteria and address three key questi...
Development of a thermostable Cre/lox-based gene disruption system and in vivo manipulations of the megaplasmid pTT27 in Thermus thermophilus HB27 [0.03%]
热稳定性Cre/洛克斯基因敲除系统的发展及模式生物Thermus thermophilus HB27中基因组大片段操作改造研究
Keiichiro Hiratsu,Tatsuo Nunoshiba,Yoichiro Togawa et al.
Keiichiro Hiratsu et al.
We previously reported the development of a Cre/lox-based gene disruption system for multiple markerless gene disruption in Thermus thermophilus; however, it was a time-consuming method because it functioned at 50 °C, the minimum growth te...
Intercellular transfer of plasmid DNA between in vitro cultured HEK293 cells following transient transfection [0.03%]
体外瞬时转染的HEK293细胞间质DNA的转移
Christoph Gerdes,F Buket Basmanav
Christoph Gerdes
Gene overexpression by transient transfection of in vitro cultured model cell lines with plasmid DNA is a commonly used method for studying molecular aspects of human biology and pathobiology. However, there is accumulating evidence suggest...
Variation in the plasmid backbone and dif module content of R3-T33 Acinetobacter plasmids [0.03%]
R3-T33阿克曼菌质粒的质粒骨架和dif模块内容的差异性
Stephanie J Ambrose,Ruth M Hall
Stephanie J Ambrose
The predominant type of plasmids found in Acinetobacter species encode a Rep_3 initiation protein and many of these carry their accessory genes in dif modules. Here, available sequences of the 14 members of the group of Rep_3 plasmids typed...
Ian Dewan,Hildegard Uecker
Ian Dewan
The length of a plasmid is a key property which is linked to many aspects of plasmid biology. When distributions of plasmid lengths are shown in the literature, they are usually plotted with length on a logarithmic scale. However, a quantit...
pSK41/pGO1-family conjugative plasmids of Staphylococcus aureus encode a cryptic repressor of replication [0.03%]
金黄色葡萄球菌的pSK41 / pGO1-家族接合质粒编码一个复制阻抑蛋白
Alvina Sarosh,Stephen M Kwong,Slade O Jensen et al.
Alvina Sarosh et al.
The majority of large multiresistance plasmids of Staphylococcus aureus utilise a RepA_N-type replication initiation protein, the expression of which is regulated by a small antisense RNA (RNAI) that overlaps the rep mRNA leader. The pSK41/...
The extensively antibiotic resistant ST111 Acinetobacter baumannii isolate RBH2 carries an extensive mobile element complement of plasmids, transposons and insertion sequences [0.03%]
携带大量可移动元件的抗生素广泛耐药ST111不动杆菌菌株RBH2的基因组特征分析
Stephanie J Ambrose,Mehrad Hamidian,Ruth M Hall
Stephanie J Ambrose
The complete genome of RBH2, a sporadic, carbapenem resistant ST111 Acinetobacter baumannii isolate from Brisbane, Australia was determined and analysed. RBH2 is extensively resistant and the chromosome includes two transposons carrying ant...
Clinical antibiotic-resistance plasmids have small effects on biofilm formation and population growth in Escherichia coli in vitro [0.03%]
临床抗生素抗性质粒会轻微抑制大肠杆菌的菌膜形成及人口增长（体外实验）
Laura Brülisauer,Ricardo León-Sampedro,Alex R Hall
Laura Brülisauer
Antimicrobial resistance (AR) mechanisms encoded on plasmids can affect other phenotypic traits in bacteria, including biofilm formation. These effects may be important contributors to the spread of AR and the evolutionary success of plasmi...