Discovery and assembly of plasmids in the fish pathogen Tenacibaculum [0.03%]
鱼类病原菌Tenacibaculum菌的质粒鉴定及组装
Ashton N Sies,Joseph P Nowlan,Laura J Schnell et al.
Ashton N Sies et al.
Members of the marine bacterial genus Tenacibaculum cause disease in finfish and outbreaks result in significant animal harm and losses in aquaculture around the globe. Plasmids have not been previously identified in Tenacibaculum, but long...
Rep_3 plasmids that carry orfX found in Acinetobacter species harbour dif modules [0.03%]
产酸克雷伯菌中携带orfX的Rep_3质粒含有dif模块
Stephanie J Ambrose,Carol H Pong,Ruth M Hall
Stephanie J Ambrose
Plasmids found in Acinetobacter species are not found in other Gram-negative species. There are many distinct plasmid types and the majority encode a Rep_3 family RepA replication initiation protein. Among these, a number were known to carr...
Beth Traxler,Eva Top
Beth Traxler
Stephanie J Ambrose,Ruth M Hall
Stephanie J Ambrose
SGI1 and its many variant forms are integrative mobilizable elements that rely on IncA or IncC plasmids for transfer functions. However, the coexistence of SGI1 with the plasmid is unstable in the longer term. Here, we have investigated the...
Shedding light on Klebsiella pneumoniae virulence: Engineering of broad host range bioluminescence reporter vectors for transcriptional analysis in drug resistant pathogens [0.03%]
切斯杆菌肺炎株毒力研究：耐药病原菌转录分析用广宿主范围生物发光报告载体的构建
Dakshayini G Chandrashekarappa,Mia E Van Allen,X Renee Bina et al.
Dakshayini G Chandrashekarappa et al.
In this work, we report the construction of four bacterial luciferase-based promoter probe vectors with an expanded set of selectable markers, designed to facilitate their use in antibiotic-resistant bacteria. These vectors contain the low-...
Mikkel M Liljegren,João A Gama,Pål J Johnsen et al.
Mikkel M Liljegren et al.
Plasmids can impact the evolution of their hosts, e.g. due to carriage of mutagenic genes, through cross-talk with host genes or as result of SOS induction during transfer. Here we demonstrate that plasmids can affect the level of microinde...
Characterization and functional insights of the novel RC-type plasmid pAnox1 from Anoxybacillus gonensis 05S15 [0.03%]
来自芽单胞菌属厌氧谷氏细菌05S15的新型RC型质粒pAnox1的表征及其功能研究
Gamze Cubukci,Hatice Ayyildiz,Kadriye Inan Bektas et al.
Gamze Cubukci et al.
The plasmid pAnox1, isolated from Anoxybacillus gonensis 05S15, was sequenced and characterized as a circular, double-stranded DNA molecule of 1592 base pairs with a GC content of 40.01 %. Despite its cryptic nature and small genome, bioinf...
miRNA heterologous production in bacteria: A systematic review focusing on the choice of plasmid features and bacterial/prokaryotic microfactory [0.03%]
基于细菌的外源性microRNA异源生产：以质粒特性选择及细菌/原核生物微流控反应器为重点的系统综述
Nyelson da Silva Nonato,Leandro Silva Nunes,Amanda Weege da Silveira Martins et al.
Nyelson da Silva Nonato et al.
Bacteria, the primary microorganisms used for industrial molecule production, do not naturally generate miRNAs. This study aims to systematically review current literature on miRNA expression systems in bacteria and address three key questi...
Development of a thermostable Cre/lox-based gene disruption system and in vivo manipulations of the megaplasmid pTT27 in Thermus thermophilus HB27 [0.03%]
热稳定性Cre/洛克斯基因敲除系统的发展及模式生物Thermus thermophilus HB27中基因组大片段操作改造研究
Keiichiro Hiratsu,Tatsuo Nunoshiba,Yoichiro Togawa et al.
Keiichiro Hiratsu et al.
We previously reported the development of a Cre/lox-based gene disruption system for multiple markerless gene disruption in Thermus thermophilus; however, it was a time-consuming method because it functioned at 50 °C, the minimum growth te...
Intercellular transfer of plasmid DNA between in vitro cultured HEK293 cells following transient transfection [0.03%]
体外瞬时转染的HEK293细胞间质DNA的转移
Christoph Gerdes,F Buket Basmanav
Christoph Gerdes
Gene overexpression by transient transfection of in vitro cultured model cell lines with plasmid DNA is a commonly used method for studying molecular aspects of human biology and pathobiology. However, there is accumulating evidence suggest...