Sylwia Bloch,Natalia Lewandowska,Grzegorz Węgrzyn et al.
Sylwia Bloch et al.
Bacteriophages play an essential role in the transferring of genes that contribute to the bacterial virulence and whose products are dangerous to human health. Interestingly, phages carrying virulence genes are mostly temperate and in contr...
Susana Brom,Elisabeth Grohman,Matxalen Llosa
Susana Brom
Quantifying plasmid dynamics using single-cell microfluidics and image bioinformatics [0.03%]
基于单细胞微流控和图像生物信息学的质粒动力学量化研究
J C R Hernandez-Beltran,J Rodríguez-Beltrán,A San Millán et al.
J C R Hernandez-Beltran et al.
Multicopy plasmids play an important role in bacterial ecology and evolution by accelerating the rate of adaptation and providing a platform for rapid gene amplification and evolutionary rescue. Despite the relevance of plasmids in bacteria...
Construction of a novel CRISPRi-based tool for silencing of multiple genes in Mycobacterium tuberculosis [0.03%]
一种用于同时沉默结核分枝杆菌多个基因的新CRISPR干扰技术的构建
Nisheeth Agarwal
Nisheeth Agarwal
Due to lipid-rich cell wall, slow growth and pathogenic nature, it is difficult to manipulate Mycobacterium tuberculosis (Mtb) genome by conventional tools. Recently we have introduced a novel CRISPRi approach for repression of genes in myc...
The ever-expanding tcp conjugation locus of pCW3 from Clostridium perfringens [0.03%]
来自产气荚膜梭菌的pCW3上不断扩增的Tcp缀合位点
Sarah A Revitt-Mills,Thomas D Watts,Dena Lyras et al.
Sarah A Revitt-Mills et al.
The spore-forming, anaerobic Gram positive pathogen Clostridium perfringens encodes many of its disease-causing toxins on closely related conjugative plasmids. Studies of the tetracycline resistance plasmid pCW3 have identified many of the ...
Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida [0.03%]
恶臭假单胞菌中新型双诱导基因（过）表达系统的构建
Rahul Gauttam,Aindrila Mukhopadhyay,Steven W Singer
Rahul Gauttam
Pseudomonas putida is a widely used host for metabolic engineering and synthetic biology. However, the use of P. putida has been hampered by the availability of a limited set of expression vectors for producing heterologous proteins. To wid...
The cell-penetrating YopM protein-functionalized quantum dot-plasmid DNA conjugate as a novel gene delivery vector [0.03%]
一种新型基因传递载体：细胞穿入YopM蛋白功能化量子点-质粒DNA缀合物
Özge Uğurlu,Fırat Barış Barlas,Serap Evran et al.
Özge Uğurlu et al.
Non-viral gene delivery systems have great potential for safe and efficient gene therapy, while inefficient cellular and nuclear uptake remain as the major hurdles. Novel approaches are needed to enhance the transfection efficiency of non-v...
Plasmidome of an environmental Acinetobacter lwoffii strain originating from a former gold and arsenic mine [0.03%]
来源于金和砷矿的环境酸质菌洛夫蒂氏菌株的质粒群簇分析
Tomasz Walter,Joanna Klim,Marcin Jurkowski et al.
Tomasz Walter et al.
Emerging important Acinetobacter strains commonly accommodate a plethora of mobile elements including plasmids of different size. Plasmids, apart from encoding modules enabling their self-replication and/or transmission, can carry a diverse...
Formation of new PHE plasmids in pseudomonads in a phenol-polluted environment [0.03%]
被酚污染的环境中的假单胞菌新PHE质粒的形成
Eve Elken,Eeva Heinaru,Merike Jõesaar et al.
Eve Elken et al.
Several years ago, a laboratory-constructed plasmid with a single-component phenol monooxygenase gene (pheBA operon) flanked by two IS elements was released to a phenol-polluted area. During the following years, we found in the test area wi...
Tailor-made sRNAs: a plasmid tool to control the expression of target mRNAs in Pseudomonas putida [0.03%]
用于铜绿假单胞菌目标mRNA表达调控的定制sRNA质粒工具
Patrícia Apura,Margarida Saramago,Alexandra Peregrina et al.
Patrícia Apura et al.
Pseudomonas putida is a highly attractive production system for industrial needs. However, for its improvement as a biocatalyst at the industrial level, modulation of its gene expression is urgently needed. We report the construction of a p...