Novel conditional plasmids regulated by chemical switches provide versatile tools for genetic engineering in Escherichia coli [0.03%]
新型化学开关调控的条件性质粒为大肠杆菌基因工程提供了多功能工具
André Riedl,Simone Gruber,Zsolt Ruzsics
André Riedl
Engineering bacterial genomes or foreign DNA cloned as bacterial artificial chromosomes (BACs) relies on usage of helper plasmids, which deliver the desired tools transiently into the bacteria to be modified. After the anticipated action is...
Structures bounded by directly-oriented members of the IS26 family are pseudo-compound transposons [0.03%]
由IS26家族直接定向的成员限定的结构是假复合转座子
Christopher J Harmer,Carol H Pong,Ruth M Hall
Christopher J Harmer
Antibiotic resistance genes are often found in structures bounded by copies of IS26, IS257/IS431 or IS1216 that resemble compound (or composite) transposons. However, because of the mechanisms used by IS26 family members, namely that they f...
Antimicrobial resistance gene shuffling and a three-element mobilisation system in the monophasic Salmonella typhimurium strain ST1030 [0.03%]
单相鼠伤寒沙门氏菌ST1030株中抗微生物耐药基因的重排和三元件动员系统
M Oliva,C Calia,M Ferrara et al.
M Oliva et al.
In this study we describe the genetic elements and the antimicrobial resistance units (RUs) harboured by the Salmonella Typhimurium monophasic variant 1,4,[5],12:i:- strain ST1030. Of the three identified RUs two were chromosomal, RU1 (IS26...
Evolution and dissemination of L and M plasmid lineages carrying antibiotic resistance genes in diverse Gram-negative bacteria [0.03%]
携带抗性基因的L和M质粒在不同革兰氏阴性菌中的起源与传播
Grace A Blackwell,Emma L Doughty,Robert A Moran
Grace A Blackwell
Conjugative, broad host-range plasmids of the L/M complex have been associated with antibiotic resistance since the 1970s. They are found in Gram-negative bacterial genera that cause human infections and persist in hospital environments. It...
Incorporating the plasmidome into antibiotic resistance surveillance in animal agriculture [0.03%]
将质粒组学纳入到动物农业抗生素耐药性监测中
N Ricker,B S Spoja,N May et al.
N Ricker et al.
Mobile genetic elements (MGE) carrying resistance genes represent a unique challenge to risk assessment and surveillance of antimicrobial resistance (AMR). Yet determining the mobility of resistance genes within animal microbiomes is essent...
Sylwia Bloch,Natalia Lewandowska,Grzegorz Węgrzyn et al.
Sylwia Bloch et al.
Bacteriophages play an essential role in the transferring of genes that contribute to the bacterial virulence and whose products are dangerous to human health. Interestingly, phages carrying virulence genes are mostly temperate and in contr...
Susana Brom,Elisabeth Grohman,Matxalen Llosa
Susana Brom
Quantifying plasmid dynamics using single-cell microfluidics and image bioinformatics [0.03%]
基于单细胞微流控和图像生物信息学的质粒动力学量化研究
J C R Hernandez-Beltran,J Rodríguez-Beltrán,A San Millán et al.
J C R Hernandez-Beltran et al.
Multicopy plasmids play an important role in bacterial ecology and evolution by accelerating the rate of adaptation and providing a platform for rapid gene amplification and evolutionary rescue. Despite the relevance of plasmids in bacteria...
Construction of a novel CRISPRi-based tool for silencing of multiple genes in Mycobacterium tuberculosis [0.03%]
一种用于同时沉默结核分枝杆菌多个基因的新CRISPR干扰技术的构建
Nisheeth Agarwal
Nisheeth Agarwal
Due to lipid-rich cell wall, slow growth and pathogenic nature, it is difficult to manipulate Mycobacterium tuberculosis (Mtb) genome by conventional tools. Recently we have introduced a novel CRISPRi approach for repression of genes in myc...
The ever-expanding tcp conjugation locus of pCW3 from Clostridium perfringens [0.03%]
来自产气荚膜梭菌的pCW3上不断扩增的Tcp缀合位点
Sarah A Revitt-Mills,Thomas D Watts,Dena Lyras et al.
Sarah A Revitt-Mills et al.
The spore-forming, anaerobic Gram positive pathogen Clostridium perfringens encodes many of its disease-causing toxins on closely related conjugative plasmids. Studies of the tetracycline resistance plasmid pCW3 have identified many of the ...