Label-free naked-eye aptasensor for detection of Cytochrome c- mediated apoptosis drug screening [0.03%]
无需标签的裸眼适配子传感器检测细胞凋亡药物筛选中的细胞色素c
Maryam-Sadat Karimi,Yasaman-Sadat Borghei,Saman Hosseinkhani
Maryam-Sadat Karimi
Apoptosis is a tightly regulated biochemical process essential for cellular homeostasis, in which the mitochondrial release of Cytochrome c (Cyt c) represents an early and irreversible hallmark. Reliable detection of Cyt c is therefore of g...
Carolina S Silva,Gabriella R Nascimento,Paulo E O Cruz et al.
Carolina S Silva et al.
Nucleic acid detection methods leveraging Cas9, Cas12, and Cas13 enzymes have recently been widely integrated with isothermal amplification techniques, particularly Loop-Mediated Isothermal Amplification (LAMP), to develop CRISPR-based diag...
Digital PCR as a potential reference measurement procedure to support monkeypox virus/Orthopoxvirus external quality assessment schemes [0.03%]
数字PCR作为参考测量程序支持猴痘病毒/正痘病毒外部质量评估方案的一种潜在方法
Samreen Falak,Jörn Beheim-Schwarzbach,Alexander Hübner et al.
Samreen Falak et al.
Since May 2022, a wave of several thousand cases of monkeypox Virus (MPXV) infections has been reported across Europe. In the beginning of this outbreak, reference material (RM) and reference measurement procedure (RMP) were lacking. We dev...
A simple and accurate high throughput evaluation method for larval zebrafish xenografts [0.03%]
一种简单准确的斑马鱼外植体高通量评价方法
Ting Liu,Yao Liang,An-Qi Cai et al.
Ting Liu et al.
The advantages of short assay duration, low cost, and high-throughput make larval zebrafish xenografts a popular in vivo model in cancer research, especially in large-scale screening of anti-tumor drug and target identification. Recently, t...
Ghazaleh Dadashizadeh,Stephanie Wojtowicz-Piotrowski,Fadi Khalaf et al.
Ghazaleh Dadashizadeh et al.
The stromal vascular fraction (SVF) of adipose tissue is a heterogeneous cell population with significant regenerative, paracrine, and immunomodulatory potential, positioning it as a promising therapeutic platform in regenerative medicine a...
The transformative role of single-cell analysis in multifactorial disorders research [0.03%]
单细胞分析在多因素疾病研究中的变革性作用
Chih-Yang Wang,Ching-Chung Ko,Sachin Kumar et al.
Chih-Yang Wang et al.
Multifactorial inherited disorders (MIDs) arise from complex interactions between polygenic risk and environmental exposures, presenting major challenges for mechanistic discovery, patient stratification, and targeted therapy development. W...
Digital choices as emotional Mirrors: Mapping psychosocial profiles through smartphone app usage patterns [0.03%]
数字选择作为情感镜子：通过智能手机应用程序使用模式映射心理社会特征
Min Hee Lee,Kyung Han You,Jaehee Cho
Min Hee Lee
This study examines how distinct patterns of smartphone application usage relate to psychosocial indicators, specifically anger, depression, and self-esteem, while identifying user clusters based on behavioral profiles. A two-stage analytic...
Comparison of RNA extraction methods from kidney organoids encapsulated in alginate-norbornene [0.03%]
封装在海藻酸酐-降冰片烯中的肾脏类器官的RNA提取方法比较
Helen Kearney,Virginie Joris,Lorenzo Moroni et al.
Helen Kearney et al.
The encapsulation of kidney organoids within hydrogels provides a biomimetic environment that enhances their structural and functional relevance for disease modeling and drug screening. However, the presence of hydrogel matrices poses a maj...
Streamlining bispecific antibody production in E. coli with scFv-Constant domain fusions [0.03%]
利用-scFv-恒定区融合简化E. coli中双特异性抗体的生产
Angela Chiew Wen Ch&#x;ng,Yee Siew Choong,Theam Soon Lim
Angela Chiew Wen Ch&#x;ng
The single-chain Fragment variable (scFv) antibody format is a common format used for phage display. The solubility of identified scFv clones in phage format in instances does not translate the same solubility as independent recombinant pro...
Protocol for partial heart transplantation of the pulmonary valve in a swine model: surgical technique, postoperative care, assessments and outcomes [0.03%]
猪肺瓣部分心脏移植术的操作方法、术后护理和评估及预后协议标准
Anshaal Furrukh,Herra Javed,Mohamed Refaat Ahmed Zaghw et al.
Anshaal Furrukh et al.
Piglets serve as an excellent model for cardiovascular research due to the anatomic and physiological similarities between the porcine and human heart. This study establishes an improved protocol for performing partial heart transplantation...