Reflectance-based optical sensor for real-time detection and quantification of biofilms in complex matrices [0.03%]
一种反射式光学传感器 实现复杂基质中生物膜的实时检测与定量分析
Sonali Rana,Lata Sheo Bachan Upadhyay,Nikhil Kumar
Sonali Rana
Biofilm detection is essential for maintaining public health and environmental integrity. Conventional detection techniques, including tube assays, microtiter plate assays, and microscopy, though effective, exhibit several limitations such ...
Determining optimal sgRNA coverage and screening duration for pooled CRISPR screens: A quantitative framework [0.03%]
确定用于池CRISPR筛选的最优sgRNA覆盖范围和筛选持续时间：一个定量框架
Tao Xu,Zhenju Guo,Yinghao Li et al.
Tao Xu et al.
CRISPR-based loss-of-function screening has emerged as a powerful tool for systematically characterizing gene functions. However, standardized quantification metrics for sgRNA coverage-a critical parameter determining genome-wide screening ...
Targeted next-generation sequencing techniques: From primer design to sophisticated library construction [0.03%]
目标导向的下一代测序技术：从引物设计到复杂的文库构建
Maxim Koryukov,Regina Mikheeva,Andrey Kechin
Maxim Koryukov
Targeted Next-Generation Sequencing (tNGS) represents a cornerstone technology in modern clinical diagnostics and biological research. Widely adopted by clinical laboratories, tNGS provides a robust and cost-effective alternative to whole-g...
Detection of allergen-specific IgE in sera from pediatric patients with food allergy using AlphaCL [0.03%]
应用AlphaCL检测食物过敏儿童血清中特异性IgE抗体
Yuki Koga,Tomoharu Yokooji,Teruaki Matsui et al.
Yuki Koga et al.
Background: Detection of serum allergen-specific IgE antibodies (sIgE) that can cross-link the high-affinity IgE receptor (FcεRI) is crucial for diagnosing IgE-mediated food allergy (FA). We previously developed an ampli...
Impact of thermal treatment on genomic DNA integrity and quantification bias in digital PCR [0.03%]
热处理对数字PCR中基因组DNA完整性及定量偏差的影响
Da Som Kim,Taejin Shin,Sae Rom Hong et al.
Da Som Kim et al.
Preserving DNA integrity, defined as intact strands and correct sequence context, is crucial to the reliability of measurements and interpretations in molecular methods. The denaturation temperature of genomic DNA is influenced by several f...
TEAMSTER: End-to-end pipeline for generation, processing, and automated quantitative image analysis of 3D tumour spheroids for nanobiology [0.03%]
团队sters：用于生成、处理和自动化定量分析三维肿瘤球体的端到端管道，以进行纳米生物学研究
Linda Barbieri,Andrea Banfi,Stefania Garbujo et al.
Linda Barbieri et al.
Three-dimensional (3D) tumour spheroids are widely used as physiologically relevant in vitro models to study tumour biology, therapeutic responses, and the distribution of drugs and nanomaterials. However, experimental workflows for spheroi...
Multi-pathway feature-level interpretability in MHC-i antigen presentation via concept-based modeling [0.03%]
基于概念的模型在MHC-I抗原呈递中的多途径特征级可解释性研究
Piyush Borole,Denise Boulanger,Ajitha Rajan
Piyush Borole
Accurate prediction of MHC-I antigen presentation is central to neoantigen discovery and immunotherapy development. Although recent deep-learning based predictors achieve high accuracy, most operate as black-box models, which limits interpr...
Identification of black carbon in placental tissue using scanning electron microscopy and energy dispersive X-ray fluorescence [0.03%]
利用扫描电子显微镜和能量色散X射线荧光鉴别胎盘组织中的黑碳
Garvita Parikh,Pratik Chhapia,Prasenjit Maity et al.
Garvita Parikh et al.
Currently, there are very few standardized and developed analysis methods for detecting the deposition of black carbon (BC) in human tissues, and they have certain limitations. A non-destructive and robust method has been developed for iden...
A high-content imaging workflow to screen for molecules that reduce cellular uptake of α-synuclein preformed fibrils [0.03%]
一种高内涵成像筛选 workflow，用于筛选可降低细胞摄取α-突触核蛋白原纤维的小分子
Wolfgang E Reintsch,Andrea I Krahn,Chanshuai Han et al.
Wolfgang E Reintsch et al.
A classical pathological hallmark of many neurodegenerative diseases is the formation of protein-rich aggregates and inclusions. In Parkinson's disease (PD), α-synuclein (α-syn) constitutes a major protein component of pathological inclus...
Advances in biosensor technologies for early diagnosis of preeclampsia through molecular biomarkers [0.03%]
基于分子生物标志物的子痫前期早期诊断的生物传感器技术新进展
Biyas Aurora Nania Nevada,Salma Nur Zakiyyah,Clianta Yudin Kharismasari et al.
Biyas Aurora Nania Nevada et al.
Preeclampsia is a pregnancy complication marked by hypertension and proteinuria occurring after 20 weeks of gestation, and it is the primary cause of maternal and neonatal morbidity and mortality globally. The primary issue in its managemen...