CRISPR/Cas12a-based tag-free fluorescent biosensor using G-quadruplex specific thioflavin T for detection of oncogenic microRNAs [0.03%]
基于G-四链体特异性底物ThT的无标签CRISPR / Cas12a荧光生物传感器检测致癌microRNA
Sepideh Hassibian,Masoomeh Esmaelpourfarkhani,Mona Alibolandi et al.
Sepideh Hassibian et al.
MicroRNAs (miRs) are central regulators of tumor initiation and progression, and their aberrant expression patterns have been identified as clinically valuable biomarkers for the early diagnosis of malignancies and prognostic evaluation. He...
Analysis of DNA double-strand breaks induced by DNA damaging agents in trophoblast organoids [0.03%]
胎盘类器官中由DNA损伤诱导的双链断裂分析
Sawako Adachi,Asuka Tanabe,Yoshiyuki Tsukamoto et al.
Sawako Adachi et al.
DNA double-strand break (DSB) is one of the most genotoxic lesions, and unrepaired DSBs can lead to chromosomal aberrations and eventually cause carcinogenesis, ageing and dysfunctions of organs. Therefore, studies of DNA repairs and DNA da...
A pipeline for quantifying cell damage in whole-slide fluorescence images of skeletal muscle [0.03%]
用于量化骨骼肌整张荧光图像中细胞损伤的管道系统
Marisa Sargent,Alastair W Wark,Arjan Buis
Marisa Sargent
Quantitative fluorescence imaging of formalin-fixed paraffin-embedded (FFPE) tissue is often limited by intensity heterogeneity, endogenous autofluorescence, and fixation-induced artifacts. Together, these factors reduce analytic accuracy a...
Identification of transgene insertion sites via short- and long-read whole genome sequencing [0.03%]
基于短读长和长读长全基因组测序的外源基因插入位点鉴定方法
Lew Kaplan,Sierra J Edgerton,Brennan D Mahoney et al.
Lew Kaplan et al.
Transgenic mouse lines are essential to uncover organ or system level genotype-phenotype relationships. The generation of such lines via transgene addition may lead to the insertion into unknown genomic loci potentially leading not only to ...
Europium(III)-driven red photoluminescent sensor for ultra-sensitive and reliable assay of sildenafil citrate in pure form, pharmaceutical formulations and Bio-fluids [0.03%]
一种超灵敏可靠的检测纯品、药剂配方及生物流体中西地那非的红色光致发光传感器
Hazim M Ali,Abd El-Naby I Essawy,Tarek Ahmed Seaf Elnasr et al.
Hazim M Ali et al.
Lanthanide complexes, particularly Eu3+ β-diketonates, offer narrow-band, long-lived red emission ideally suited to sensitive fluorescence assays in complex media. The Eu(TTA)3(H2O)2 chelate combines efficient near-UV excitation, robust em...
Synergistic strategies combining nanomaterial-based oxygen modulation and immunotherapy for hepatocellular carcinoma [0.03%]
基于纳米材料的氧气调节与免疫疗法联合治疗肝细胞癌的协同策略
Chunzhong Qiao,Tao Cheng,Junsheng Li
Chunzhong Qiao
Hepatocellular Carcinoma (HCC) remains one of the leading causes of death across the world due to late diagnosis, unresponsiveness to treatment, and poor prognosis. The presence of a hypoxic tumor microenvironment (TME) plays an essential r...
Tapabrata Chakraborti,Subhadip Basu
Tapabrata Chakraborti
Membrane-Mediated Methods [0.03%]
膜介导法
Chiman Song,Jun-Seok Lee,Eun Joo Roh et al.
Chiman Song et al.
Combined in vitro optical and in silico docking approaches: evaluating kinetic and thermodynamic parameters in the taurine-albumin complex [0.03%]
结合体外光学和分子对接模拟计算方法评价牛黄酸-白蛋白复合物的热力学及动力学参数
Eman A Alabdullkarem,Naifa Alenazi,Rhunda Hayek et al.
Eman A Alabdullkarem et al.
This study investigates the interaction between human serum albumin (HSA) and taurine (2‑aminoethanesulfonic acid) in aqueous solution using a multi-technique biophysical approach. The interaction of taurine (as an additive, supplement, or...
Sequencing of uterine fluid is a promising tool for predicting endometrial receptivity: a proof-of-concept study [0.03%]
子宫液测序是预测子宫内膜接受性的一种有前景的工具：概念验证研究
Miaoxin Chen,Dan Zhou,Yangyun Zou et al.
Miaoxin Chen et al.
Background: Endometrial receptivity is crucial to a successful pregnancy. Although molecular markers of endometrial receptivity can be obtained through endometrium biopsy, the prediction method is invasive and delayed. He...