Virus-Like Particles: From Fundamental Concepts to Cutting-Edge Applications - A Comprehensive Review of Production, Engineering, and Clinical Translation [0.03%]
病毒样颗粒：从基本概念到前沿应用——生产、工程和临床转化的全面综述
Mohammad Sadegh Lotfi,Saeed Jafari,Mohammad Razmyeki et al.
Mohammad Sadegh Lotfi et al.
Virus-like particles (VLPs) are non-infectious nanoparticles that resemble native viruses, and are highly immunogenic because of their repetitive surface geometry and efficient lymph node drainage. Although their effectiveness in prophylact...
Emerging frontiers in genome editing: From CRISPR to next-generation technologies [0.03%]
基因编辑新兴前沿：从CRISPR到下一代技术
Saurabh Mishra,Samrah Rehan,Ahmad Mujtaba Barekzai et al.
Saurabh Mishra et al.
Genome editing has revolutionized molecular biology. It offers precise modification of genetic material across diverse organisms. This review outlines the evolution of genome editing technologies from homologous recombination to advanced Cl...
Comprehensive evaluation of reference miRNA for extracellular miRNA quantification in CKD [0.03%]
慢性肾脏病患者外泌体中参考miRNA的选择及表达特征分析
Anvesha Srivastava,Mark Maienschein-Cline,Ana Pabalan et al.
Anvesha Srivastava et al.
MicroRNAs (miRNAs) are promising biomarkers and therapeutic targets; however, reliable extracellular miRNA quantification by RT-qPCR depends on appropriate normalization, and no universal endogenous reference has been established. This stud...
Robust carotid artery perfusion protocol for turtle brain fixation enabling multiscale imaging from magnetic resonance to super-resolution microscopy [0.03%]
一种鲁棒的颈动脉灌注协议 用于使龟脑固定化 可实现从磁共振到超分辨率显微成像的多尺度成像
Yasser Medlej,Ahmadali Lotfinia,Michaela Klinkmann et al.
Yasser Medlej et al.
Reptiles, particularly turtles, are increasingly recognized as valuable models in comparative and evolutionary neuroscience; however, standardized methods for brain perfusion and fixation remain lacking. Here, we present the first detailed,...
Pan Zheng,Xiangxiang Zeng
Pan Zheng
Reflectance-based optical sensor for real-time detection and quantification of biofilms in complex matrices [0.03%]
一种反射式光学传感器 实现复杂基质中生物膜的实时检测与定量分析
Sonali Rana,Lata Sheo Bachan Upadhyay,Nikhil Kumar
Sonali Rana
Biofilm detection is essential for maintaining public health and environmental integrity. Conventional detection techniques, including tube assays, microtiter plate assays, and microscopy, though effective, exhibit several limitations such ...
Determining optimal sgRNA coverage and screening duration for pooled CRISPR screens: A quantitative framework [0.03%]
确定用于池CRISPR筛选的最优sgRNA覆盖范围和筛选持续时间：一个定量框架
Tao Xu,Zhenju Guo,Yinghao Li et al.
Tao Xu et al.
CRISPR-based loss-of-function screening has emerged as a powerful tool for systematically characterizing gene functions. However, standardized quantification metrics for sgRNA coverage-a critical parameter determining genome-wide screening ...
Targeted next-generation sequencing techniques: From primer design to sophisticated library construction [0.03%]
目标导向的下一代测序技术：从引物设计到复杂的文库构建
Maxim Koryukov,Regina Mikheeva,Andrey Kechin
Maxim Koryukov
Targeted Next-Generation Sequencing (tNGS) represents a cornerstone technology in modern clinical diagnostics and biological research. Widely adopted by clinical laboratories, tNGS provides a robust and cost-effective alternative to whole-g...
Detection of allergen-specific IgE in sera from pediatric patients with food allergy using AlphaCL [0.03%]
应用AlphaCL检测食物过敏儿童血清中特异性IgE抗体
Yuki Koga,Tomoharu Yokooji,Teruaki Matsui et al.
Yuki Koga et al.
Background: Detection of serum allergen-specific IgE antibodies (sIgE) that can cross-link the high-affinity IgE receptor (FcεRI) is crucial for diagnosing IgE-mediated food allergy (FA). We previously developed an ampli...
Impact of thermal treatment on genomic DNA integrity and quantification bias in digital PCR [0.03%]
热处理对数字PCR中基因组DNA完整性及定量偏差的影响
Da Som Kim,Taejin Shin,Sae Rom Hong et al.
Da Som Kim et al.
Preserving DNA integrity, defined as intact strands and correct sequence context, is crucial to the reliability of measurements and interpretations in molecular methods. The denaturation temperature of genomic DNA is influenced by several f...