Optimization of 13C stable isotope labeling for the study of tricarboxylic cycle intermediates in mouse models [0.03%]
优化研究小鼠模型三羧酸循环中间体的13C稳定同位素标记法
Jarrod Laro,Monica Ness,Joseane Godinho et al.
Jarrod Laro et al.
The tricarboxylic acid cycle (TCA), also known as the Krebs Cycle or the citric acid cycle, is an essential metabolic pathway involved in energy production that is often impacted by disease, making it of key interest to identify effective, ...
ResDeepGS: A deep learning-based method for crop phenotype prediction [0.03%]
基于深度学习的作物表型预测方法ResDeepGS
Chaokun Yan,Jiabao Li,Qi Feng et al.
Chaokun Yan et al.
Genomic selection (GS) is a breeding technique that utilizes genomic markers to predict the genetic potential of crops and animals. This approach holds significant promise for accelerating the improvement of agronomic traits and addressing ...
Michaelis-Menten kinetics of RasGAP proteins by a rapid fluorescence-based assay [0.03%]
基于快速荧光分析的RasGAP蛋白的米氏动力学研究
Maxum E Paul,Kimberly J Vish,Titus J Boggon
Maxum E Paul
Ras small GTPases are essential for a wide range of cellular processes. These proteins cycle between the GDP-loaded and GTP-loaded states, and the actions of GTPase activating proteins (GAPs) are necessary to stimulate Ras-mediated GTP hydr...
Unveiling the Pathogenicity of Allosteric Protein Mutations via Multifaceted Feature Ensembling [0.03%]
基于多维特征整合的别构蛋白突变体致病性研究
Huiling Zhang,Xijian Li,Junwen Huang et al.
Huiling Zhang et al.
Allosteric proteins play a central role in biological processes and systems. Identifying the biological impact of mutations on allosteric proteins and the phenotypes they influence during disease initiation and progression presents a signif...
Deciphering cellular heterogeneity: Breakthroughs and prospects of single-cell-level SERS analysis in precision medicine [0.03%]
揭秘细胞异质性：单细胞表面增强拉曼光谱分析在精准医疗中的突破与前景
Biqing Chen,Jiayin Gao,Haizhu Sun et al.
Biqing Chen et al.
Single-cell surface-enhanced Raman scattering (SERS) has emerged as a powerful tool for precision medicine owing to its label-free detection, ultrasensitivity, and unique molecular fingerprinting. Unlike conventional bulk analysis, it enabl...
Michaela Beranova,Petra Grossova,Jiri Demuth et al.
Michaela Beranova et al.
Chemically modified oligonucleotides (ONs) are essential tools in molecular biology, diagnostics, and therapeutics. Strain-promoted azide-alkyne cycloaddition (SPAAC) offers an efficient and bioorthogonal method for ON functionalization. Wh...
AI-augmented prediction of high-risk PINK1 variants associated with Parkinson's disease: integrating multilayered bioinformatics, MD simulation, and deep learning [0.03%]
基于多层生物信息学、分子动力学模拟和深度学习的与帕金森病相关的高风险PINK1变异体的AI增强预测
Hafeez Ur Rehman,Dawood Ahmad Warraich,Abdur Rehman et al.
Hafeez Ur Rehman et al.
Parkinson's disease is a prevalent neurodegenerative disease, in which genetic mutations in many genes play an important role in its pathogenesis. Among these, a mutation in the PINK1 gene, a mitochondrial-targeted serine/threonine putative...
Capturing G protein-coupled receptors into native lipid-bilayer nanodiscs using new diisobutylene/maleic acid (DIBMA) copolymers [0.03%]
基于二异丁烯/马来酸(DIBMA)共聚物将G蛋白偶联受体捕捉到天然脂质双层纳米盘中
Ci Chu,Carolyn Vargas,Maria Carolina Barbosa et al.
Ci Chu et al.
Many membrane proteins, including G protein-coupled receptors (GPCRs), are susceptible to denaturation when extracted from their native membrane by detergents. Therefore, alternative methods have been developed, including amphiphilic copoly...
Efficient RNA nucleotide encoding enhances the accurate prediction of ac4C modifications [0.03%]
高效的RNA核苷酸编码可增强ac4C修饰的准确预测能力
Na Li,Xiao Wang,Ming Zeng et al.
Na Li et al.
RNA N4-acetylcytidine (ac4C) modification plays a vital role in gene regulation and cellular function. Accurate identification of ac4C sites is essential for elucidating their biological significance. However, existing prediction methods st...
Rapid in vitro and computer-aided method for assessing synergistic interactions between NSAIDs and analgesics [0.03%]
快速体外和计算机辅助评估非甾体抗炎药与止痛药之间协同作用的方法
Fernando Silva,Gustavo Costa,Francisco Veiga et al.
Fernando Silva et al.
Pain is a complex phenomenon that plays a significant role in various diseases, influencing both the physical and psychological well-being of individuals. In clinical practice, combining nonsteroidal anti-inflammatory drugs (NSAIDs) with an...