Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis [0.03%]
单分子技术研究结核杆菌RNA聚合酶转录机制
Wenxia Lin,Omar Herrera-Asmat,Alexander B Tong et al.
Wenxia Lin et al.
Single molecule methods have become prevalent tools in elucidating molecular processes across various life science fields over the past three decades, driving breakthroughs in understanding their underlying molecular mechanisms. In our stud...
Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels [0.03%]
蚊虫监测期间乌苏图病毒的检测和测序：用于低水平识别的多种检测试剂盒和技术
Stephen Findlay-Wilson,Isobel Everall,Amy Nettlemill et al.
Stephen Findlay-Wilson et al.
Background: Usutu virus (USUV) is a medically important flavivirus linked to significant mortalities within bird populations and an increasing association with human infections, particularly within immunocompromised indiv...
Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children [0.03%]
人工智能驱动的数字医疗平台在越南儿童口腔健康行为和牙菌斑评估中的实验验证
Ji-Na Lim,Vo Truong Nhu Ngoc,Tuan Manh Vu et al.
Ji-Na Lim et al.
Background: Oral health among children in developing countries, including Vietnam, remains a significant public health concern. Innovative approaches leveraging artificial intelligence AI-based digital health platforms ma...
Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions [0.03%]
zeta电位：一种高效且低成本的探究细胞表面相互作用的方法
Aakash Gupta,Weizheng Wang,Joy Zhao et al.
Aakash Gupta et al.
Background: Monitoring and quantifying cell-surface interactions is important for drug discovery and understanding the physiological and pathological activities of cells. While techniques like Enzyme-linked immunosorbent ...
An automated workflow for quantifying the formation of synuclein aggregates in human dopaminergic neurons [0.03%]
人源多巴胺能神经元中突触核蛋白聚集形成的自动化定量流程
Chanshuai Han,Emmanuelle Nguyen-Renou,Faiza Benaliouad et al.
Chanshuai Han et al.
Parkinson's disease (PD) is a neurodegenerative disorder characterized by alpha-synuclein (α-syn) aggregates termed Lewy bodies. To model PD pathology in vitro, preformed fibrils of α-syn (PFFs), which can be taken up by cells, provide a ...
A novel methodological framework for the assessment of the neural control of the shoulder using high-density surface electromyography [0.03%]
一种新的使用高密度表面肌电图评估肩关节神经控制的方法框架
J Greig Inglis,Silvia Rio,Hélio V Cabral et al.
J Greig Inglis et al.
Background: Complex shoulder function relies on coordinated activation of small and large muscles, including the deltoid, pectoralis major, trapezius, and latissimus dorsi. However, detailed knowledge of their neuromuscul...
A fluorescence-based high-content imaging workflow for multi-parametric lipid droplet phenotyping via Oil Red O fluorescence [0.03%]
基于荧光的高内涵成像工作流程通过油红O荧光进行多参数脂滴表型分析
Shaojun Yan,Yuqin Yao,Congying Liu et al.
Shaojun Yan et al.
Progression of Metabolic-associated Fatty Liver Disease (MAFLD) involves excessive accumulation of intrahepatic lipid droplets (LDs). However, cost-effective, high-throughput imaging for detailed, multi-parametric LDs quantification at the ...
Streamlined isolation of enriched axoplasm from primary dissociated neuronal cultures [0.03%]
来自原代分离神经元培养的富集轴突质的简化分离方法
Sabta Alarcón,Andrés Fuentes,Elizabeth Carrazana et al.
Sabta Alarcón et al.
Axons are highly specialized neuronal compartments that rely on local molecular regulation to support growth, maintenance, and synaptic function. The cytoplasmic contents of axons, collectively referred to as the axoplasm, constitute a bioc...
CRISPR-enhanced ELISA based on ssDNA-triggered Cas13a-Csm6 cascade for sensitive profiling of inflammatory biomarkers in sports-related osteoarthritis among martial arts athletes [0.03%]
基于单链DNA触发的Cas13a-Csm6级联的CRISPR增强型ELISA在与运动相关的骨关节炎中灵敏检测武术运动员炎症生物标志物
Yaoshu Li,Chengyang Xu,Jiang Li
Yaoshu Li
Sports-related osteoarthritis (OA), which is common among martial arts athletes exposed to repetitive high-impact loading, is closely associated with complex inflammatory responses. Sensitive detection of inflammatory biomarkers is therefor...
Modeling 3D Glioblastoma: A review of spheroid culture techniques and standardization challenges [0.03%]
三维胶质母细胞瘤模型：类器官培养技术及标准化难题综述
Leonor M Castro,Lara Paiva,Inês Lobo et al.
Leonor M Castro et al.
Glioblastoma (GB) is among the most aggressive and treatment-resistant cancers, highlighting the urgent need for physiologically relevant preclinical models. Traditional two-dimensional (2D) cultures fall short in replicating the complex mi...