Opto-fiber biosensor for tetrodotoxin detection using novel optical fiber structure functionalized with MoS2 and MWCNTs [0.03%]
一种新型二氧化钼和多壁碳纳米管功能光纤结构的河豚毒素检测试纸传感器
Xiaoxuan Liu,Yiyan Xie,Xinlong Zhou et al.
Xiaoxuan Liu et al.
Tetrodotoxin (TTX) is one of the most potent neurotoxins, with complex structures and analgesic effects. This toxin exists mainly in aquatic organisms, such as pufferfish, and has high biological activity. In this study, a novel three-taper...
New candidate reference measurement procedure for NRAS p.Q61R quantification by digital PCR [0.03%]
用于数字PCR检测NRAS p.Q61R的新候选参考测量程序
Jessica Petiti,Sabrina Caria,Laura Revel et al.
Jessica Petiti et al.
Accurate and comparable quantification of somatic mutations is essential for precision oncology, as clinical decision-making increasingly relies on the quantification of molecular biomarkers. Despite major technological advances, inter-labo...
Mapping protein-DNA interactions across biological scales: principles, scalability and applications [0.03%]
跨生物尺度的蛋白质-DNA相互作用的映射：原则、可扩展性和应用
Guang Song,Kangning Li,Yanjia Sun
Guang Song
Mapping protein-DNA interactions (PDIs) is essential for understanding transcriptional regulation and chromatin organization. Experimental approaches now range from in vitro assays that characterize intrinsic DNA-binding specificity to chro...
Establishing a standard workflow for fluorescence-based nanoparticle tracking analysis for the reliable quantification of extracellular vesicles [0.03%]
建立基于荧光的纳米颗粒追踪分析的标准工作流程，以可靠地量化细胞外囊泡
Yewon Han,Minseop Kim,Zayakhuu Gerelkhuu et al.
Yewon Han et al.
Fluorescence-based nanoparticle tracking analysis (Fl.NTA) enables selective detection and quantification of labelled nanoparticles. However, the lack of a standardized workflow has hindered the reproducibility and comparability of concentr...
Quantitative single-cell analysis of PML-RARα oncogene-induced DNA damage along cell cycle progression [0.03%]
基于细胞周期进程的PML-RARα癌基因诱导的DNA损伤的单细胞定量分析
Silvia Scalisi,Greta Paternò,Gaetano Ivan Dellino et al.
Silvia Scalisi et al.
Oncogene activation represents a critical initiating event in tumorigenesis, promoting replication stress and impairing genome stability. The PML-RARα oncogene, responsible for acute promyelocytic leukemia, disrupts the organization of pro...
Cilia SubQ: a modular suite of semi- and fully automated pipelines for analysis of primary cilia and ciliary subdomains [0.03%]
Cilia SubQ：一个用于原生纤毛及其亚结构分析的半自动和全自动管道模块化套件
Elizabeth Menzel,Karama Hamdi,Gracie Hoffman et al.
Elizabeth Menzel et al.
Primary cilia are conserved, antenna-like sensory organelles that protrude from the surface of most vertebrate cells. Their core function is to detect extracellular cues and convert them into downstream signaling, essential for embryogenesi...
Projective invariant of surface ratio: application to pupil measurement through simulations and proof-of-concept recordings [0.03%]
曲面有理不变量在瞳孔测量中的应用：基于仿真和原理验证性记录
Pierre M Daye,Pierre Pouget
Pierre M Daye
Despite the usefulness of pupil size assessment as a proxy for subjects' state of mind in clinical and neuroscientific studies, its measure remains confined to non-ecological, controlled experiments where the subject fixates a stationary ta...
A quantitative radiographic framework for longitudinal monitoring of additively manufactured biodegradable scaffolds with graded tantalum reinforcement [0.03%]
定量放射框架用于纵向监测具有分级钽增强的添加剂制造可降解支架长期变化规律
Ömer Burak Istanbullu,Ömer Barışkan Yasan
Ömer Burak Istanbullu
Purpose: This study proposes a standardized, quantitative radiographic evaluation framework for assessing the detectability and imaging fidelity of biodegradable scaffolds, enabling longitudinal X-ray-based monitoring thr...
An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions [0.03%]
一种用于评估血浆和血清累积暴露于解冻条件下的简便吸光度平板读取器测定法
Nilojan Jehanathan,Ishmael A Quansah,Schuyler Kremer et al.
Nilojan Jehanathan et al.
The integrity of human plasma and serum (P&S) is crucial for reliable biomarker discovery and validation, but it is affected by preanalytical exposure to thawed conditions (temperatures > -30 °C). The limited number of existing quality con...
EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples [0.03%]
EC-iSHCR：一种在不同动物样本中进行原位滚环扩增的快速方法
Yasuhiro Kozono,Kyohei Mikami,Shunta Yorimoto et al.
Yasuhiro Kozono et al.
The in situ hybridization chain reaction (isHCR) visualizes RNA across multiple spatial scales, from organs to subcellular structures, in diverse samples. We previously proposed a rapid protocol, EC-isHCR, for Drosophila embryos and ovaries...