Structural divergence in N-terminal domains of AAA proteases paraplegin (SPG7) and FtsH indicates a key structural function in complex formation [0.03%]
AAA蛋白酶paraplegin（SPG7）和FtsH的N端结构域的结构分歧表明其在复合物形成中的关键结构功能
James G Hyatt,Neil G Paterson,Juliette M Devos et al.
James G Hyatt et al.
AAA proteases are hexameric ATP-dependent metallopeptidases that perform crucial proteolytic activities within prokaryotic and eukaryotic membranes. Structurally, protomers are comprised of catalytically active C-terminal domains that are a...
Wei Li,Qingting Liu,Jinhong Li et al.
Wei Li et al.
DNA methyltransferase DNMT3A is a key enzyme responsible for establishing DNA methylation patterns during mammalian development. T-cell leukemia/lymphoma 1 A (TCL1A) is a proto-oncogene expressed mainly in embryonic and fetal tissues, as we...
Kolaviron influences the calf thymus DNA conformational stability and structural dynamics: a multi-spectroscopic and molecular dynamics anatomization [0.03%]
基于多光谱和分子动力学的 kolaviron 对小牛胸腺 dna 影响的研究
Oluseyi V Ochima,Adejoke N Kolawole,Babatunde A Falese et al.
Oluseyi V Ochima et al.
Kolaviron, flaunted for its anticancer potential, has driven our exploration of its mechanism of interaction with DNA, and further shedding light on its therapeutic promise. Herein, the binding efficacy and thermodynamics of kolaflavanone, ...
Biotin protein ligase C-terminal domain arginines are critical for ATP interaction & biotinyl-5'-AMP formation [0.03%]
生物素蛋白连接酶C端结构域的精氨酸对于ATP相互作用及生物素氨磷酸形成的critical性研究
Sonika Bhatnagar,Debodyuti Sadhukhan,Manoj Kumar Rajak et al.
Sonika Bhatnagar et al.
Electrostatic interactions between arginines and phosphates are central to numerous biological processes. Here, using an integrated approach combining mutagenesis, activity measurements, molecular dynamics (MD) simulations, and NMR, we demo...
Automated LN₂ refill device for uninterrupted cryoFIB-SEM operations [0.03%]
用于不间断cryo-FIB/SEM操作的自动化液氮填充装置
Imre Gonda,Daniel Junker,Fabian Eggimann et al.
Imre Gonda et al.
Due to recent technological advances, in situ structural cell biology is becoming a high throughput microscopy technique as all the steps of the workflow, from sample preparation to data analysis, are executed faster, more reliable and more...
Cryo-EM structure of a single-chain β1-adrenoceptor - AmpC β-lactamase fusion protein [0.03%]
单链β1肾上腺素能受体-氨基糖青霉素酶融合蛋白的冷冻电镜结构
Gabriella Collu,Inayathulla Mohammed,Aleix Lafita et al.
Gabriella Collu et al.
The insertion of fusion proteins has enabled the crystallization of a wide range of G-protein-coupled receptors. Here, we adapted this engineering strategy to cryo-electron microscopy (cryo-EM). We inserted the soluble protein AmpC β-lacta...
Transient calcite precursor phases in Bryozoa: Smittina cervicornis and Myriapora truncata from the Mediterranean [0.03%]
地中海Smittina cervicornis和Myriapora truncata中钙质前体相的瞬态性研究
Dorrit E Jacob,Grit Benedix,Richard Wirth et al.
Dorrit E Jacob et al.
Myriapora truncata and Smittina cervicornis, two common Bryozoa in the Mediterranean Sea form erect calcitic colonies with similar ultrastructures characterised by platy and granular calcite grains. At the submicron scale, the mineralized p...
Structural enzymological studies of multifunctional enzyme, type-1 (MFE1) with the 2E-decenoyl-CoA and 2E,4E-decadienoyl-CoA substrates: The regeneration of the dehydrogenase catalytic site is the rate limiting step of its combined reactions [0.03%]
以2E-癸烯酰辅酶A和2E，4E-癸二烯酰辅酶A为底物的多功能1型（MFE1）异构还原酶的结构酶学研究：脱氢酶催化位点再生是其复合反应的速度控制步骤
Shruthi Sridhar,Werner Schmitz,Mikael Widersten et al.
Shruthi Sridhar et al.
The rat peroxisomal multifunctional enzyme, type-1 (RnMFE1) is a monomeric enzyme with two active sites, which catalyze the second and third reaction of the β-oxidation cycle, being the 2E-enoyl-CoA hydratase (ECH) and the 3S-hydroxyacyl-C...
MLAC: MicroED-assisted ligand structure analysis in complexes and its application to hERG-ligand complexes [0.03%]
基于复合物的MicroED辅助配体结构分析及其在人乙酰胆碱酯酶-配体复合物中的应用
Keita Tanaka,Yasuomi Miyashita,Kenji Iwasaki et al.
Keita Tanaka et al.
Accurate determination of ligand structures in protein-ligand complexes is essential for elucidating molecular recognition mechanisms and advancing structure-based drug discovery. Cryogenic electron microscopy (cryo-EM) has emerged as a pow...
Ultrastructural evidence of autophagy-related processes and mitochondrial remodeling in the myxozoan parasite Henneguya piaractus [0.03%]
黏体动物寄生虫Henneguya piaractus中与自噬和线粒体重建相关的超微结构证据
Kassia Roberta Hygino Capodifoglio,Fernanda Schneberger,Antonio Augusto Mendes Maia et al.
Kassia Roberta Hygino Capodifoglio et al.
Henneguya piaractus is a myxozoan parasite infecting the gills of Piaractus mesopotamicus, yet its cellular biology remains poorly understood. Here, we investigated mitochondrial organization and the occurrence of autophagy-related processe...