Pharmacokinetics of IgG1 monoclonal antibodies produced in humanized Pichia pastoris with specific glycoforms: a comparative study with CHO produced materials [0.03%]
人源化毕赤酵母中特定糖型IgG1单克隆抗体的药代动力学与CHO细胞系统表达产物比较研究
Liming Liu,Andy Stadheim,Lora Hamuro et al.
Liming Liu et al.
A glycoengineered Pichia pastoris host was used to produce an IgG1 with either afucosylated N-glycosylation (afucosylated biantennary complex) or without N-glycosylation (N297A) while a wild type P. pastoris host was used to produce an IgG1...
Methods to select suitable fetal bovine serum for use in quality control assays for the detection of adventitious viruses from biological products [0.03%]
用于生物制品外来病毒检测试验的质量控制方法及选择适宣胎牛血清的方法
Takashi Kozasa,Hiroshi Aoki,Nao Nakajima et al.
Takashi Kozasa et al.
Production of biological products, especially vaccines, usually requires materials derived from animals, and there are always risks that animal pathogens derived from these materials could contaminate the final products. Detection of advent...
Biochemical and biological characteristics of cross-reacting material 197 CRM197, a non-toxic mutant of diphtheria toxin: use as a conjugation protein in vaccines and other potential clinical applications [0.03%]
CRM197的生物化学和生物学特性：一种白喉毒素非毒性突变体及其作为疫苗偶联蛋白和其他潜在临床应用的可能性
Michael Bröker,Paolo Costantino,Lisa DeTora et al.
Michael Bröker et al.
The biochemical and biological characteristics of CRM(197) are reviewed. Polysaccharide protein conjugate vaccines represent an important technological advancement that allowed for protection against dangerous diseases in vulnerable populat...
Granulocyte-macrophage colony stimulating factor: Evaluation of biopharmaceutical formulations by stability-indicating RP-LC method and bioassay [0.03%]
粒细胞-巨噬细胞集落刺激因子：稳定性指示反相液相色谱法及生物检定法评价其生物制剂型式
Diogo Paim Leal,Ricardo Bizogne Souto,Renato Schutkoski et al.
Diogo Paim Leal et al.
The granulocyte-macrophage colony stimulating factor (GM-CSF) is a cytokine that regulates the proliferation and differentiation of hematopoietic cells and activates granulocytes and macrophages. A reversed-phase liquid chromatography (RP-L...
Feasibility of the use of ELISA in an immunogenicity-based potency test of anthrax vaccines [0.03%]
酶联免疫吸附试验在炭疽疫苗免疫原性效力测定中应用的可能性研究
Alicia Jiménez-Alberto,Patricia Parreiras,Juan Castelán-Vega et al.
Alicia Jiménez-Alberto et al.
Complexities of lethal challenge animal models have prompted the investigation of immunogenicity assays as potency tests of anthrax vaccines. An ELISA was used to measure the antibody response to protective antigen (PA) in mice immunized on...
Effect of bone sialoprotein on proliferation and osteodifferentiation of human bone marrow-derived mesenchymal stem cells in vitro [0.03%]
骨唾液蛋白对体外人骨髓间充质干细胞增殖及成骨分化的影响
Bing Xia,Jie Wang,Lida Guo et al.
Bing Xia et al.
We performed this study to investigate the effects of recombinant human bone sialoprotein (BSP) on the proliferation and osteodifferentiation of human BMSCs(hBMSCs). The hBMSC cultures were divided into 4 groups: control group, 10(-10) M BS...
Different effects of (L)-arginine on the heat-induced unfolding and aggregation of proteins [0.03%]
(L)-精氨酸对热诱导蛋白质变性及聚集的影响不同
Andrejus Cirkovas,Jolanta Sereikaite
Andrejus Cirkovas
Circular dichroism spectroscopy was used to study the effect of l-arginine on the temperature related unfolding and aggregation of three growth hormones, i.e. human, porcine and mink growth hormones, and human interferon-α2b. (L)-arginine ...
A comparative study of monosaccharide composition analysis as a carbohydrate test for biopharmaceuticals [0.03%]
生物药品碳水化合物检测中单糖组成分析的比较研究
Akira Harazono,Tetsu Kobayashi,Nana Kawasaki et al.
Akira Harazono et al.
The various monosaccharide composition analysis methods were evaluated as monosaccharide test for glycoprotein-based pharmaceuticals. Neutral and amino sugars were released by hydrolysis with 4-7N trifluoroacetic acid. The monosaccharides w...
Philip D Minor
Philip D Minor
The results of tests of the neurovirulence of three yellow fever vaccine preparations of different lineages are presented. Two preparations that have been used to make vaccines of acceptable safety and efficacy gave very similar results. A ...
The Wildbad Kreuth initiative: European current practices and recommendations for optimal use of blood components [0.03%]
Wildbad Kreuth倡议：欧洲血药用成分当前应用及最佳使用建议
Karin Berger,Harvey G Klein,Rainer Seitz et al.
Karin Berger et al.
With the aging population in Europe it is anticipated that the growing demand for blood products will not be met by the estimated supply. Therefore up-to-date recommendations for optimal administration of blood products in hemotherapy are n...