Tailoring a DMA-based cryopreservation protocol with anti-freeze (glycol)proteins for commercial and native breeds of chicken [0.03%]
基于DMA的含有抗冻（糖）蛋白的玻璃化冷冻保存方法在地方品种和商业品种鸡中的优化
Berenice Bernal,Éva Váradi,Árpád Drobnyák et al.
Berenice Bernal et al.
This study evaluated the effects of antifreeze proteins AFPI, AFPIII, and AFGP during fresh processing, and freezing-thawing of chicken semen from a commercial broiler breed (BB) and the local breed Yellow Hungarian (YH), using 0.6 mol/L DM...
Can a tropical lizard (Xenosaurus platyceps) survive freezing temperatures? [0.03%]
热带蜥蜴（Xenosaurus platyceps）能耐受冰点温度吗？
Natalia Fierro-Estrada,Fausto R Méndez-de la Cruz,Oswaldo Téllez-Valdes
Natalia Fierro-Estrada
Thermoregulation is crucial for lizards, which can employ stenothermic or eurythermic strategies and active or passive thermoregulation. Some organisms that live at low temperatures use physiological freeze avoidance (supercooling) or freez...
Similar developmental outcomes of salamander larvae produced with fresh and cryopreserved sperm: implications for amphibian conservation [0.03%]
冷冻和新鲜精子产生的蝾螈幼虫具有相似的发育结果：对两栖动物保护的意义
Shaina Lampert,Isabella Burger,Devin Chen et al.
Shaina Lampert et al.
Sperm cryopreservation is an important tool for the genetic management of threatened amphibian species within conservation breeding programs. Yet, little is known about the growth and development of offspring generated using frozen-thawed s...
Age leaves a molecular mark: altered A-Kinase Anchor Protein 4 (proAKAP4) abundance and sperm cell quality drive fertility decline in Bali bulls (Bos sondaicus) [0.03%]
年龄在分子上留下了印记：A-蛋白激酶锚定蛋白4（proAKAP4）含量变化和精子细胞质量下降导致巴厘公牛不育
Irma Octaria Sari,Berlin Pandapotan Pardede,Mokhamad Fakhrul Ulum et al.
Irma Octaria Sari et al.
Reproductive performance in bulls is strongly influenced by age-related factors, which can lead to functional and molecular deterioration of sperm even when conventional semen parameters remain within acceptable ranges. This study aimed to ...
William A Rowe,Chris Rowe Taitt
William A Rowe
The application of artificial intelligence in cryopreservation: Technological advances and future challenges [0.03%]
人工智能在冷冻保存中的应用：技术进步与未来挑战
Xingyue Lei,Liqun He,Gang Zhao
Xingyue Lei
This review systematically sorts out the latest research progress and application status of artificial intelligence (AI) technology in the field of cryopreservation, with a focus on its action mechanisms in aspects such as protocol optimiza...
Effective cryopreservation of western capercaillie (Tetrao urogallus) semen using dimethylsulphoxide [0.03%]
使用二甲基亚砜有效冻存西部黑琴鸡（Tetrao urogallus）精液
Alberto Gómez-Crespo,Artur Kowalczyk,Julián Santiago-Moreno et al.
Alberto Gómez-Crespo et al.
This work examines the use of Me2SO as a cryoprotective agent (CPA) for capercaillie semen, comparing it with the use of dimethyformamide (DMF). Thirty semen samples were collected from seven males, diluted and divided into two aliquots bef...
Impact of cryopreservation on pollen viability and morphological integrity of five Indian Crinum species [0.03%]
五种印度蜘蛛兰属植物的冷冻保存对花粉活力和形态完整性的的影响
Harshid Pulparambil,P E Rajasekharan,N S Pradeep
Harshid Pulparambil
Crinum species are ornamental plants of high horticultural value and medicinal importance, known for their bioactive alkaloids with neurological and anticancer properties. Sustainable use and genetic conservation of Crinum require reliable ...
An effective method for recovering rat spermatogonial stem cells from frozen rat testis cells [0.03%]
一种从冻存大鼠睾丸细胞中有效回收大鼠精原干细胞的有效方法
Fan Yang,Yaru Li,Mary R Avarbock et al.
Fan Yang et al.
Cryopreservation of testis tissue, including spermatogonial stem cells (SSCs), underpins a potential method of fertility preservation for prepubertal males undergoing gonadotoxic treatments. A key understudied factor in the recovery of high...
The histologic and molecular alterations during the cold storage of the ascending human colon [0.03%]
冷储存期间升结肠的组织学和分子改变
Jasmine Bagge,Gustav Hagberg,Anna Ermund et al.
Jasmine Bagge et al.
Inclusion of a colon segment in visceral grafts is increasing as this may improve water and electrolyte absorption and ultimately improve recipients' renal function. In contrast with the vast knowledge on the ischemia/reperfusion injury in ...