In memoriam: James Lovelock (1919-2022) - A scientific life in cryobiology and beyond [0.03%]
讣告：詹姆斯·拉夫洛克（1919-2022）——一位在低温生物学等领域作出贡献的科学家
Sanaz Hemmatibardehshahi,Celina Phan,Jason P Acker
Sanaz Hemmatibardehshahi
Charles A Elder,Ivan Klbik,Ali M Alkafaji et al.
Charles A Elder et al.
Biologics, including vaccines, therapeutic proteins, blood products, and cell therapies, are susceptible to degradation during drying and therefore require the incorporation of protective additives (lyoprotectants) into the processing formu...
Developing a cryopreservation protocol for the forest-forming seaweed Phyllospora comosa as a tool for restoration [0.03%]
一种用于形成森林的海藻Phyllospora comosa 的冷冻保存方法的发展及其在修复中的应用研究
Catalina A Musrri,Georgina V Wood,Adriana Vergés et al.
Catalina A Musrri et al.
Global declines in seaweed forests are reducing genetic diversity and, consequently, the resilience of these habitats to changing environmental conditions. To conserve genetic diversity and secure these important coastal habitats, new prese...
Droplet-vitrification cryopreservation of shoot tips of the endangered plant Lilium tsingtauense [0.03%]
濒危植物百合种苗的液滴玻璃化冷冻保存研究
Jiajia Li,Jiangping Song,Xiaohui Zhang et al.
Jiajia Li et al.
This study established an efficient droplet-vitrification cryopreservation protocol for the endangered plant Lilium tsingtauense. Through optimization, the best conditions were determined as follows: shoot tips were precultured in 0.3 M suc...
The effect of whole-body cryostimulation treatments on the profile of selected adipokines and sirtuins in middle-aged individuals with varying degrees of body fatness [0.03%]
全身低温刺激治疗对不同体脂水平的中年人血清中选者性脂肪因子和Sirtuins的影响
Adrianna Dzidek,Olga Czerwińska-Ledwig,Roxana Zuziak et al.
Adrianna Dzidek et al.
Whole-body cryostimulation (WBC), a widely utilized intervention involving exposure to extremely low temperatures, elicits a range of physiological responses. Growing evidence suggests that WBC may serve as a complementary, non-pharmacologi...
Metabolic adaptations in the hypothermic myocardium: Mechanisms and clinical implications [0.03%]
心室停搏期间心肌代谢的适应性改变：机制和临床意义
Huimin Wu,Fei Xiao,Yuan Zhang et al.
Huimin Wu et al.
Hypothermia is widely used in clinical practice for myocardial protection during cardiac arrest (CA), surgery, and organ preservation. However, a comprehensive understanding of how hypothermia reshapes myocardial metabolism remains lacking....
Feiyu Peng,Lixiang Liu,Yifeng Yang et al.
Feiyu Peng et al.
Sperm cryopreservation imposes osmotic stress due to the addition and removal of high-concentration cryoprotectants. Whether organic osmolytes can alleviate this stress to improve cryopreservation efficiency remains unclear. Here, we evalua...
Dose- and size-dependent effects of Fe3O4 nanoparticles on bull sperm cryopreservation [0.03%]
Fe3O4纳米颗粒对公牛精子冷冻保存的影响及剂量和尺寸依赖性效应研究
Ali Erdem Öztürk,Şeyma Dadı,Mustafa Bodu et al.
Ali Erdem Öztürk et al.
Fe3O4 nanoparticles (Fe3O4 NPs) are widely used in health sciences, including reproductive biotechnology. In reproductive systems, they have been applied in sperm cryopreservation, nano-purification, drug delivery, and toxicity assessments....
Ultra-rapid freezing yields a higher cryoresistance than the conventional slow freezing of epididymal guinea pig (Cavia porcellus) spermatozoa [0.03%]
超快速冷冻比传统慢速冷冻法能更好地保存小家猪附睾精子的受精能力
Diego A Galarza,Christian A Hernández-Lojano,Anddy J Salinas-Ulloa et al.
Diego A Galarza et al.
Ultra-rapid (UR) freezing represents a promising alternative to conventional-slow (CS) freezing of spermatozoa in certain species, with the potential to reduce post-thaw cryodamage. This study evaluated the quality of guinea pig (Cavia porc...
Cryopreservation of hormonally induced sperm of the common toad (Bufo bufo) with a cushion centrifugation step: a preliminary study [0.03%]
激素诱导的普通蟾蜍精子的冷冻保存（带缓冲离心步骤）初步研究
Natalia V Shishova,Svetlana A Kaurova,Victor K Uteshev
Natalia V Shishova
One of the most important methods for preserving amphibian biodiversity is sperm cryobanking, in particular cryopreservation of spermic urine obtained by hormonal induction of spermiation. However, for bufonids, cryopreserved sperm obtained...