Imaging sweetness: Rapid permeation of sucrose into rice callus cells as a component of cryoprotectant solutions [0.03%]
甜味成像：作为冷冻保护剂溶液成分的蔗糖快速渗透到水稻愈伤组织细胞中
Albert Ochoa-Castillo,Gayle M Volk,Nancy E Levinger
Albert Ochoa-Castillo
Sucrose was first included in plant vitrification solutions (PVS) in 1989 and has since remained a primary component of most formulations. Although sucrose is known to stabilize cell membranes, prevent protein denaturation, and promote cell...
Field-applicable skin tissue cryopreservation protocols for mammalian genetic banking: Evidence from Neotropical cervids [0.03%]
哺乳动物基因库的皮肤组织 cryo保存技术：来自新热带鹿科动物的证据
Eluzai Dinai Pinto Sandoval,Luciana Diniz Rola,Juan Daniel Jaramillo-Hernández et al.
Eluzai Dinai Pinto Sandoval et al.
Cryopreserved genetic resources from threatened species face substantial logistical challenges, particularly in field settings where laboratory infrastructure is limited. This study evaluated three simplified cryopreservation protocols for ...
Shortened warming preserves cellular resilience and accelerates post-warming development in human blastocysts: A propensity score-matched study [0.03%]
短时冻存保持人类囊胚细胞的耐受性和加速解冻后的发育：倾向性匹配研究
Jae Kyun Park,Jieun Won,Yunmi Jeon et al.
Jae Kyun Park et al.
Conventional multi-step warming protocols (MWP) for vitrified blastocysts are labor-intensive and susceptible to handling variability. This study evaluated whether a shortened warming protocol (SWP) could support post-warming recovery and d...
Genetic and epigenetic alterations in human gametes following cryopreservation: human evidence and insights from mammalian animal models [0.03%]
人类配子冷冻保存后的遗传和表观遗传改变：人类证据及哺乳动物模型的启示
Maryna Petrushko,Oksana Falko,Julia Gontar et al.
Maryna Petrushko et al.
Cryopreservation of gametes is an integral component of assisted reproductive technologies and fertility preservation; however, exposure to ultra-low temperatures may be associated with structural, genetic, and epigenetic alterations releva...
Comparing blastocyst recovery and blebbing following one-step warming under osmotic conditions with and without biopsy [0.03%]
在渗透条件下一步复苏与或不伴随活检对囊胚回收率及肿胀的影响比较研究
Yamato Mizobe,Yuji Orita,Tokiko Iwakawa et al.
Yamato Mizobe et al.
We investigated the optimal thawing solution for one-step warming and assessed the efficacy of the one-step warming method for blastocysts subjected to biopsy. Vit Kit-Warm NX Thawing Solution (TS; Irvine Scientific, Santa Ana, CA, USA; osm...
Isochoric supercooling improves preservation of hepatic spheroid viability and structure compared to 4°C cold storage [0.03%]
等容过冷与4℃条件下肝脏类器官体外保存的比较研究
Marie L Dicker,Anthony N Consiglio,Yun Weng et al.
Marie L Dicker et al.
Cell spheroids and organoids possess three-dimensional cell-cell interactions that more closely recapitulate in vivo tissue architecture than compared to conventional monolayer culture. Pluripotent stem cell-derived organoids demonstrate hi...
Enhancing aquaculture efficiency: Development of a reliable cryopreservation protocol for Leiarius marmoratus [0.03%]
石斑鱼养殖效率的提升：雷阿里斯玛罗布鲁氏鱼可靠冷冻保存方法的发展研究
Eduardo Thomé Nicoleti,Rômulo Batista Rodrigues,Jhony Lisboa Benato et al.
Eduardo Thomé Nicoleti et al.
Leiarius marmoratus is a freshwater catfish widely used in Brazilian aquaculture for hybrid production with Pseudoplatystoma spp., representing an important genetic resource for sustainable aquaculture. This study aimed to establish a stand...
Optimization of ultra-low temperature cryopreservation and post-thaw reproductive performance of Ruditapes philippinarum sperm [0.03%]
菲律宾蛤仔超低温冷冻保存及解冻后繁殖性能的优化研究
Xinyu Ma,Bingyan Sun,Yan Jin et al.
Xinyu Ma et al.
Cryopreservation serves as a vital technique for long-term germ preservation of aquatic species, and post-thaw sperm viability is essential to successful germplasm conservation. Here, we optimized the ultra-low temperature cryopreservation ...
PD-1/PD-L1 inhibition after cryoablation is associated with improved tumor control and immunosuppressive microenvironment remodeling in Lewis lung adenocarcinoma [0.03%]
液氮冷冻消融后 PD-1/PD-L1 抑制与Lewis肺癌腺癌的免疫微环境重塑和肿瘤控制改善有关
Qi Liu,Shuo Zhang,Xuxin Chen et al.
Qi Liu et al.
Cryoablation (Cryo) is used for local tumor control in lung cancer patients who are not candidates for surgical resection, but its effects on distant tumor growth remain incompletely understood. The present study evaluated the effects of Cr...
Antifreeze protein type I preserves mitochondrial activity in in vivo-derived sheep embryos after cryopreservation: a stage-dependent effect [0.03%]
羊胚胎冷冻保存后线粒体活性的抗冻蛋白I阶段依赖性保护作用
Thais A Oliveira,Mariana P P Guimarães,Priscila Del&#x;Aguila-Silva et al.
Thais A Oliveira et al.
This study assessed, in sheep, the effects of antifreeze protein (AFP) type I on redox balance at different embryonic stages by measuring mitochondrial activity (MITO), reactive oxygen species (ROS), and glutathione (GSH) levels in the same...