TMT-based proteomic analysis reveals key proteins involved in cryoinjury of Nubian goat spermatozoa [0.03%]
基于TMT的蛋白质组学分析揭示了努比亚公羊精子冷损伤的关键蛋白
Jianwei Zou,Lili Wei,Yishan Liang et al.
Jianwei Zou et al.
Cryopreservation is a common assisted reproductive technology in goat breeding, but it often impairs sperm quality and fertilizing ability. This study aimed to explore the effects of cryopreservation on sperm quality and protein abundance p...
Interrupted cooling protocols in cryopreservation: A review of fundamentals, methods and experimental outcomes for cells in suspension [0.03%]
中断的冷却程序在冷冻保存中的应用：悬浮细胞的基本原理、方法和实验结果综述
Elham Ashrafi,Janet A W Elliott
Elham Ashrafi
Interrupted cooling protocols are effective techniques used in cryobiology to study how cells respond to the freeze/thaw processes. By interrupting either rapid or slow cooling processes within specific sub-zero temperature ranges, research...
Cryotherapy reduces functional impairment and oxidative damage after sciatic nerve injury in rats [0.03%]
cryotherapy减轻大鼠坐骨神经损伤后的功能障碍和氧化应激损伤
Jaíne Dalmolin,Andrezza B V Furtado,Diane D Hartmann et al.
Jaíne Dalmolin et al.
Peripheral Nerve Injury (PNI) has a multifactorial etiology and results in functional impairment in both motor and sensory behavior, which can be reversed by appropriate treatment strategies. As PNI results in an inflammatory response, cons...
Evaluation of CIRBP and ONECUT2 for fatal hypothermia identification - a pilot study [0.03%]
CIRBP和ONECUT2鉴别致命性低体温的诊断价值初步研究
Gang Chen,Xiangmeng Wang,Jiaxin Qi et al.
Gang Chen et al.
Due to the lack of specificity in diagnosing death from hypothermia through anatomical and pathological examinations, it is often necessary to rule out other causes of death, such as injury or disease. Thus, finding a method that can distin...
The mechanism of optimized two-step osmo-protection in regulating the cell homeostasis of 'Light Yellow' Petunia × Calibrachoa callus [0.03%]
调控石竹目植物愈伤组织细胞稳态的优化两步渗透保护机制研究——以轻黄洋紫荆× calibration为例
Yuanshan Zhang,Guanqun Chen,Junsong Pan et al.
Yuanshan Zhang et al.
Cryopreservation is essential for long-term plant germplasm preservation, yet the mechanisms underlying cellular responses during the critical dehydration phase remain poorly understood. In this study, callus tissues of 'Light Yellow' Petun...
Permeable cryoprotectant-free ultra-rapid freezing of high-density human spermatozoa: novel catheter device and concentrated pellet approaches [0.03%]
高密度的人类精子的渗透性无冷冻保护剂的超快速冻结：新型导管装置和浓缩制剂的方法
Marzieh Torkamanpari,Azam Agha-Rhimi,Ali Nabi et al.
Marzieh Torkamanpari et al.
Sperm ultra-rapid freezing techniques cryopreserve selected spermatozoa without seminal plasma using non-permeable cryoprotectants. Existing methods are limited to cryopreserving low sperm volumes. This study aims to enhance the volume of u...
Morphological changes in ovarian follicles, stromal cells, and extracellular matrix in cryopreserved cattle ovarian tissue and the beneficial effects of Croton argyrophyllus Kunth essential oil [0.03%]
冷存小牛卵巢组织中卵泡、间质细胞和胞外基质的形态变化及阿灰笔花精油的有益作用
Paloma de Souza Santana,Francisco das Chagas Costa,Francisco Freire Caetano Filho et al.
Paloma de Souza Santana et al.
This study examined morphological changes in preantral follicles, stromal cells, and the extracellular matrix of cattle ovarian tissue vitrified in medium supplemented with Croton argyrophyllus Kunth essential oil (CaEO). Ovarian cortical s...
Cryopreservation techniques for blue catfish, Ictalurus furcatus, sperm using 2.5 mL straws [0.03%]
用于蓝猫鱼精子冷冻保存的2.5毫升细管冷冻法研究
Kyle R Wood,Brian Bosworth,Samitha S N Liyanage et al.
Kyle R Wood et al.
Our objectives were to investigate how permeating cryoprotectant agents (CPAs; dimethyl sulfoxide, methanol), non-permeating CPAs (trehalose at 100 and 300 mmol/L; lactose at 5 and 10 %), and freezing rates (-1.0, -5.0, -10, -20 °C/min) im...
State of the art and application of gamete and reproductive tissue banking in wildlife conservation [0.03%]
野生动物保护中生殖细胞和繁殖组织存库研究进展及其应用
Jennifer B Nagashima,Budhan Pukazhenthi,Nucharin Songsasen
Jennifer B Nagashima
In this Anthropocene era, we are witnessing a rapid loss of biodiversity. To date, nearly 25% of the approximately 6,000 vertebrate species assessed by the International Union for Conservation of Nature are threatened with extinction. While...
John G Baust
John G Baust