Dose-dependent effects of dimethyl sulfoxide (DMSO; Me2SO) for platelet cryopreservation: 10% Me2SO provides superior cryoprotection [0.03%]
二甲基亚砜（DMSO；Me2SO）剂量依赖性效应在血小板冷冻保存中的应用：10% Me2SO提供了更佳的冷冻保护作用
Lacey Johnson,Pearl Lei,Christopher Roan et al.
Lacey Johnson et al.
The most widely used method of platelet cryopreservation involves the addition of 5-6% dimethyl sulfoxide (DMSO; Me2SO), followed by pre-freeze centrifugation and hyperconcentration to minimize the content of Me2SO transfused. However, seve...
Cryopreservation and other preservation approaches for animal models workshop session I: Invertebrate models in biomedical research [0.03%]
动物模型保存方法研讨会第一部分：生物医学研究中的无脊椎动物模型
Christopher Fang-Yen,Daryl M Gohl,Jonathan D Hibshman et al.
Christopher Fang-Yen et al.
The ability to preserve small invertebrate organisms indefinitely at ultracold temperatures has been an important tool for biological and biomedical research. This report provides a summary of an NIH-sponsored workshop on Cryopreservation a...
Protective effects of melatonin on mouse ovarian tissue against oxidative stress and ferroptosis may involve the MT1 receptor [0.03%]
褪黑素通过MT1受体可能对氧化应激和铁死亡下的小鼠卵巢组织起到保护作用
Sheng Nan Jiang,Xin Zhang,Li Na Si et al.
Sheng Nan Jiang et al.
Ovarian cryopreservation is an important method for fertility preservation, with oxidative stress and ferroptosis being key contributors to cryopreservation-induced ovarian damage. Previous studies have shown that melatonin (MLT) protects v...
Resveratrol at low concentrations enhances sperm-oxidative stress parameters and fertilizing-hatching potential in cryopreserved turkey semen [0.03%]
低浓度白藜芦醇改善冷冻火鸡精液的氧化应激参数和受精孵化潜力
Mohammad Amin Farzane,Tohid Mohammadi
Mohammad Amin Farzane
This research explored the impact of semen extenders enhanced with resveratrol (RSV) on the quality and fertility of post-thawed turkey sperm. Semen was pooled and diluted in a glucose-based extender with RSV at concentrations of 0.01, 0.1,...
Vitalii Mutsenko,Lilia Kuleshova,Birgit Glasmacher et al.
Vitalii Mutsenko et al.
Over the past decades, cryobiology has become increasingly integrated across a variety of domains. With broad intersection, this discipline has recently also emerged as a cornerstone for advancing and clinically translating tissue-engineere...
Mechanisms of water migration and their impact on probiotic stability during freeze-drying: A comprehensive review [0.03%]
冻干过程中水分迁移机制及其对益生菌稳定性影响的全面综述
Hui Yao,Shufeng He,Tchouli Noufeu et al.
Hui Yao et al.
Vacuum freeze-drying is a critical technology for extending the shelf life of probiotics while preserving their functional viability. However, during processing, water migration and ice crystal formation can lead to substantial cellular dam...
External N-2-fluorophenyl-D-gluconamide does not affect zebrafish embryogenesis [0.03%]
外源N-2-氟苯基-D-葡糖胺对斑马鱼胚胎发育无影响
Rafay Osmani,Lucie Cavallotto,Nishaka William et al.
Rafay Osmani et al.
Cryopreservation of zebrafish embryos remains challenging due to their low tolerance for cryoprotectant exposure and their susceptibility to lethal ice formation. Ice recrystallization inhibitors (IRIs) such as N-2-fluorophenyl-D-gluconamid...
Mitigating cryodamage in ram semen using lutein-loaded chitosan nanoparticles: Insights from antioxidant and apoptotic analyses [0.03%]
使用含有 luetein的壳聚糖纳米粒子减轻公羊精子冷冻损伤：来自抗氧化和凋亡分析的视角
Ali A El-Raghi,Amer K Mohammed,Ekramy M Elmorsy et al.
Ali A El-Raghi et al.
This study aimed to investigate the impact of adding lutein-loaded chitosan nanoparticles (LU-CNPs) to a semen extender on the quality, membrane integrity, kinematic parameters, redox status, apoptosis-like changes, ultrastructural integrit...
Identification of the most stable reference gene for RT-qPCR analysis of vitrified-warmed bovine blastocysts: Validation through gene expression analysis [0.03%]
牛玻璃化冷冻解冻囊胚RT-qPCR分析中最稳定参考基因的鉴定及验证
Tae-Gyun Kim,Sung-Ho Kim,Sang-Yup Lee et al.
Tae-Gyun Kim et al.
Accurate normalization using stable reference genes (RGs) is essential for RT-qPCR, particularly in single bovine blastocysts with limited RNA. To date, RG validation under vitrification and warming conditions remains limited. This study co...
CryoClean technology: A novel closed vitrification system combining high efficiency and sterility for embryo cryopreservation [0.03%]
CryoClean技术：一种新型的封闭玻璃化系统，结合高效性和无菌性用于胚胎冷冻保存
Rongxiang Wang,Hui Yang,Hongjuan Ye et al.
Rongxiang Wang et al.
Embryo vitrification is a cornerstone of assisted reproductive technology (ART). Traditional open systems, while efficient, risk microbial contamination, whereas closed systems prioritize safety at the expense of cooling efficiency. This st...