An in-silico and experimental dual-screening strategy for robust lyoprotection of restriction endonucleases [0.03%]
用于限制性内切酶冻干保护的基于计算机和实验的双重筛选策略
Jian Zhang,Ting Cui,Shaojie Ma et al.
Jian Zhang et al.
An in-silico and experimental dual-screening lyophilization strategy was developed for long-term ambient storage of two model restriction endonucleases (REase) of XbaI (oxidation-resistant) and XhoI (oxidation-sensitive). After molecular do...
Cryopreservation of testicular tissue from pubertal dogs (Canis lupus familiaris) comparing slow freezing and solid surface vitrification methods [0.03%]
比较慢速冷冻和固体表面玻璃化法狗（灰狼）青春期睾丸组织的冻存
Inaraã Dias da Luz,Antonio Sergio Varela Junior,Gustavo Desire Antunes Gastal et al.
Inaraã Dias da Luz et al.
Cryopreservation of testicular tissue is crucial for germplasm banking in canine breeding programs, conservation of endangered canids, and veterinary oncology. However, optimal methods for canine testicular tissue remain underexplored due t...
Cryopreservation of spermatogonia from Yesso scallop, Mizuhopecten yessoensis [0.03%]
低温保存海湾扇贝（Mizuhopecten yessoensis）精原细胞
Kentaro Anayama,Taro Tsuda,Jeongwoong Yoon et al.
Kentaro Anayama et al.
The Yesso scallop is a commercially important species in the northern coastal regions of East Asia; however, its aquaculture production has recently become increasingly unstable due to global climate change. Cryopreservation of germ cells a...
Effects of trehalose, melatonin and lipid mixtures on bull sperm parameters and gene expressions during cryopreservation [0.03%]
海藻糖、褪黑激素和脂质混合物对冷冻精液品质及基因表达的影响
Vahit Ağır,Mustafa Numan Bucak,Mustafa Garip et al.
Vahit Ağır et al.
The objective of this study was to investigate the effects of trehalose and different doses of melatonin and lipid mixtures on the quality parameters of post-thaw bull sperm during cryopreservation. The ejaculates of three mature bulls were...
Ectoine-based cryopreservation of human cells: a systematic review of efficacy and safety [0.03%]
基于ectoine的细胞低温保存：功效和安全性的系统性综述
Setareh Barani,Mostafa Pournamdari,Soroush Sardari et al.
Setareh Barani et al.
Cryopreservation is crucial for research and cell therapies, but common cryoprotectants like dimethyl sulfoxide may cause toxicity. Ectoine and hydroxyectoine are natural compatible solutes proposed as safer alternatives or adjuncts. We per...
Reference microRNA selection for qPCR normalization in cryopreserved Bos taurus semen [0.03%]
牛冷冻精液miRNAs的qPCR内参照选取研究
Lucas Petitemberte de Souza,Leandro Silva Nunes,Luana Carla Salvi et al.
Lucas Petitemberte de Souza et al.
The accurate quantification of microRNA (miRNA) expression in cryopreserved bovine semen is essential for studies aiming to identify fertility biomarkers. However, reliable normalization of qPCR data remains a challenge due to the lack of v...
A guide to cryogenic refrigerant properties and selection for tissue & organ cryopreservation [0.03%]
组织与器官 cryopreservation的制冷剂属性和选择指南
Lakshya Gangwar,Dorothy Haggard,Michael L Etheridge et al.
Lakshya Gangwar et al.
Cryopreservation relies on refrigerants for achieving and sustaining ultra-low (>-150 °C) or cryogenic temperatures (
Multiscale investigation of Snomax effects on supercooling with application to high-subzero cryopreservation: Calorimetry and cryomacroscopy [0.03%]
Snomax对超低温影响的多尺度研究及其在高冷冻温度保存中的应用：热量学和冰宏观镜技术
Devarsh M Vispute,Simona Baicu,Michael J Taylor et al.
Devarsh M Vispute et al.
This is a multiscale experimental investigation to study supercooling effects in selected cryoprotective agents (CPAs), in the presence and absence of the commercial ice nucleation promoter Snomax. Microscale and macroscale experiments were...
Physiological and cellular basis of inter-species variation in freeze tolerance between Bombyx mori and Antheraea pernyi [0.03%]
家蚕和天蚕耐冻性的生理及细胞基础差异分析研究
Xiaoshuai Wang,Zaize Liu,Ao Zhu et al.
Xiaoshuai Wang et al.
Freeze tolerance varies dramatically among closely related insects, yet the underlying physiological and cellular mechanisms remain poorly understood. Here, we performed an integrative biophysical and cellular analysis comparing Bombyx mori...
Dose-dependent effects of dimethyl sulfoxide (DMSO; Me2SO) for platelet cryopreservation: 10% Me2SO provides superior cryoprotection [0.03%]
二甲基亚砜（DMSO；Me2SO）剂量依赖性效应在血小板冷冻保存中的应用：10% Me2SO提供了更佳的冷冻保护作用
Lacey Johnson,Pearl Lei,Christopher Roan et al.
Lacey Johnson et al.
The most widely used method of platelet cryopreservation involves the addition of 5-6% dimethyl sulfoxide (DMSO; Me2SO), followed by pre-freeze centrifugation and hyperconcentration to minimize the content of Me2SO transfused. However, seve...