Long-term viability of dermatophytes: a comparative evaluation of cryopreservation media [0.03%]
表皮癣菌长期存活能力的比较研究：冷冻保存介质的影响
Rita de Cassia Santos da Silva,Juliana Gomes de Souza Oliveira,Juan Diego Ribeiro de Almeida et al.
Rita de Cassia Santos da Silva et al.
Reliable long-term preservation of dermatophytes is essential for diagnostics, taxonomy, and experimental reproducibility, but simple and comparative protocols remain scarce. We evaluated viability and colony morphology of 59 clinical isola...
Temperature-metabolism relationships above and below the body fluid freezing point in the freeze-tolerant white worm, Enchytraeus albidus [0.03%]
耐冻白土蚕（Enchytraeus albidus）体液冰点上下温度-代谢关系研究
Mathias Engell Dahl Holmstrup,Johannes Overgaard,Stine Slotsbo
Mathias Engell Dahl Holmstrup
Enchytraeus albidus is a freeze-tolerant oligochaete with potential for long-term cryopreservation storage as live feed in aquaculture. In this study, we measured the metabolic rate (MR) of E. albidus across a range of temperatures (+20 to ...
Cryopreservation decreased lipopolysaccharide-induced immune response of endometrial stromal cells via inhibiting the expression of TRIF [0.03%]
冻存通过抑制TRIF表达来降低内膜间质细胞的LPS诱导免疫反应
Cuiting Yang,Yan Zhang,Chun Wang et al.
Cuiting Yang et al.
In the study, we investigated the effect of cryopreservation on the immune response of endometrial stromal cells (ESCs) to lipopolysaccharide (LPS). We exposed both frozen-thawed and untreated ESCs to LPS and measured cytokine levels as wel...
Characterizing the cryobiological response of a mouse cardiac endothelial cell line to interrupted slow cooling (graded freezing) [0.03%]
小鼠心脏内皮细胞系分级冷冻损伤特征化研究
Elham Ashrafi,Janet A W Elliott
Elham Ashrafi
Mouse cardiac endothelial cells play a critical role in cardiovascular research and disease; hence, high quality cryopreservation of this cell type is necessary to provide on-demand access to the cells, quality control, and consistent outco...
Glucose and maltose supplementation in the freezing medium of Creole rooster spermatozoa improves in vivo fertility [0.03%]
冻存雄鸡精子培养基中添加葡萄糖和麦芽糖可提高其体内受精能力
Elías Segarra Zenteno,Liliana Morocho,Karen Vásquez et al.
Elías Segarra Zenteno et al.
This study investigated the effects of glucose-maltose supplementation in freezing medium on the kinematic, viability, and fertility of Creole rooster spermatozoa. Fifty ejaculates from 10 Creole roosters were obtained in 5 weekly sessions ...
Alexis Theodorou,Karel Pomeisl,Jan Pokorný et al.
Alexis Theodorou et al.
Hydrophilic membranes are integral components in a wide range of applications, particularly in biological systems, where their negatively charged surfaces resemble those of cell membranes and influence processes such as cryopreservation. Th...
Extracellular vesicles as emerging bioactive modulators in sperm cryopreservation: Mechanisms and therapeutic potentials [0.03%]
外泌体在精子冻存中的新兴生物活性调节作用：机制和治疗潜力
Merve Deniz Tanrıkulu,Mesut Çevik,Mustafa Numan Bucak et al.
Merve Deniz Tanrıkulu et al.
Sperm cryopreservation is a cornerstone of assisted reproduction and genetic conservation, yet the freeze-thaw process induces substantial structural and functional damage to spermatozoa. Recent progress in the field has positioned extracel...
Modulation of inflammation of lipid metabolism by sesamin, forskolin, α-linolenic acid, and their combination in a zebrafish model of cryo-injury and high cholesterol diet-induced myocardial infarction, followed by atherosclerosis [0.03%]
芝麻素、佛波林、α-亚麻酸及其组合调节cryo损伤和高胆固醇饮食诱导的心肌梗死斑马鱼模型中脂质代谢炎症的作用机制研究
Abu Safana Biswas,Kamsagara Linganna Krishna,Ganavi Bethanagere Ramesha et al.
Abu Safana Biswas et al.
Cryo-injury has been a standard model used to simulate myocardial infarction (MI), which may induce reproducible myocardial necrosis under controlled conditions. In a Zebrafish (ZF) model of cryoinjury and a high-cholesterol diet (HCD), the...
Coaxial temperature controlled cryoprinting: A biomimetic technology inspired by the freezing survival mechanisms of the frog Ranasylvatica [0.03%]
受林地蛙(Rana sylvatica)冻存机制启发的同轴温控微纳直写技术
Leo Lou,Linnea Warburton,Cristina Bilbao-Sainz et al.
Leo Lou et al.
Inspired by the freeze-survival mechanism of the frog Rana sylvatica, we developed a co-axial, self-crosslinking approach to be used when fabricating frozen scaffolds. Soft biomaterial can be fabricated into scaffolds using methods such as ...
Antioxidant astaxanthin enhances cryosurvival and post-thaw functional parameters of rabbit spermatozoa [0.03%]
虾青素能提高兔精子的冻存存活率和解冻后的功能参数抗氧化剂
Paula Luque,Lenka Kuželová,Jakub Vozaf et al.
Paula Luque et al.
This study evaluated the effect of astaxanthin (AX), a potent antioxidant carotenoid, on the post-thaw quality of cryopreserved rabbit sperm. Semen samples were frozen with AX supplementation at concentrations of 0 (CONTROL), 0.5, 1, or 2 ...