Structural and Biochemical Insights into the Broad-Spectrum TET enzyme from Methanocaldococcus jannaschii reveals the basis of substrate specificity in M42 aminopeptidases [0.03%]
来源于Methanocaldococcus jannaschii的TET酶结构和生化见解揭示了M42氨肽酶底物特异性的基础
Joaquin Atalah,Hind Basbous,Gregory Effantin et al.
Joaquin Atalah et al.
TET peptidases of the M42 family are ∼500 kDa hollow dodecameric complexes ubiquitous in prokaryotes. These enzymes act as strict aminopeptidases, catalyzing the removal of N-terminal amino acids from peptides. A common feature of M42 TET ...
Missense3D-PTMdb: a web tool for visualising and exploring human genetic variants and post-translational modification sites using AlphaFold models [0.03%]
Missense3D-PTMdb：使用AlphaFold模型可视化和探索人类遗传变异和翻译后修饰位点的网络工具
Haotian Zhao,Ryan Pye,Grace Walker et al.
Haotian Zhao et al.
Only a fraction of the >11 million missense variants identified in humans has a known clinical significance. Post-translational modifications (PTMs), such as phosphorylation, glycosylation and ubiquitination, are key regulators of protein f...
MASI2.0: Insights of Microbiota Metabolic Potential from Incorporating Genomic Information on Microbiota-Active Substance Interactions [0.03%]
MASI2.0：通过将微生物组的基因组信息纳入其中来探讨微生物组代谢潜能中的活性物质相互作用的新见解
Dongyue Hou,Yuchen Liu,Hanbo Lin et al.
Dongyue Hou et al.
Extensive interactions between microbiota and active substances are health- and disease-relevant. Mechanistic understanding from genomic perspective of these interactions and potential impacts is important for biomedical and pharmaceutical ...
Integrating sequence, structure, and graph-based features for elucidating the stability of thermophilic proteins [0.03%]
集成序列、结构和图谱特征以阐明嗜热蛋白的稳定性
P Ramakrishna Reddy,Fathima Ridha,M Michael Gromiha
P Ramakrishna Reddy
Proteins from thermophilic organisms exhibit remarkable stability under extreme thermal conditions. Understanding the molecular mechanisms underlying thermostability is essential for studying protein evolution and engineering robust enzymes...
Jijie Chai
Jijie Chai
Jijie Chai earned his Ph.D. in analytical chemistry from the Institute of Materia Medica, Chinese Academy of Medical Sciences, and conducted postdoctoral research at Princeton University. In 2004, he established his independent research gro...
Cryo-EM of cardiac AL-224L amyloid reveals shared structural motifs and mutation-induced differences in λ6 light chain fibrils [0.03%]
心肌AL-224L淀粉样变的冷冻电镜显示λ6轻链纤维具有一致和变异的结构单元
Chad W Hicks,Tatiana Prokaeva,Brian Spencer et al.
Chad W Hicks et al.
In light chain amyloidosis (AL), aberrant monoclonal antibody light chains (LCs) deposit in vital organs causing organ damage. Each AL patient features a unique LC; previous cryogenic electron microscopy (cryo-EM) studies revealed different...
Transcriptome-wide RNA stability atlas Illuminates post-transcriptional control and therapeutic vulnerabilities across cancers [0.03%]
泛癌转录组范围内的RNA稳定性图谱揭示了转录后调控和治疗弱点
Yang Tong,Yuting Wang,Gerui Liu et al.
Yang Tong et al.
The steady-state abundance of mRNA is governed by the interplay between transcription and degradation, yet the contribution of RNA stability to cancer biology remains incompletely understood. Here, we systematically investigate RNA decay dy...
Influence of methionine oxidation on protein stability and association studied by free energy simulations [0.03%]
基于自由能模拟研究蛋氨酸氧化对蛋白质稳定性和缔合的影响
Tristan Alexander Mauck,Martin Zacharias
Tristan Alexander Mauck
Cellular metabolic systems but also the extracellular environment can generate reactive oxygen species that lead to oxidation of methionine (MET) and interfere with protein folding and protein-protein association. The molecular mechanism of...
The Nuclease Domain of E. coli RecBCD Helicase Regulates DNA Binding and Base Pair Melting [0.03%]
大肠杆菌RecBCD解旋酶的核酸酶结构域调控其DNA结合及碱基对变性能力
Linxuan Hao,Rui Zhang,Timothy M Lohman
Linxuan Hao
E. coli RecBCD, a hetero-trimeric helicase and nuclease, functions in double stranded (ds) DNA break repair. RecBCD possesses ATPase motor domains within both RecB (3' to 5') and RecD (5' to 3') and a nuclease domain within RecB (RecBNuc). ...
Structure-based discovery of a non-competitive FTO inhibitor bound to a cryptic site at the domain interface [0.03%]
基于结构的发现：非竞争性FTO结合剂与领域界面内的隐秘位点结合
Aayushi Singh,Francesco Pettini,Beatrice Gianibbi et al.
Aayushi Singh et al.
The fat mass and obesity-associated fatso (FTO) protein is a member of the AlkB family of dioxygenases whose overexpression links to several metabolic diseases, including obesity, type 2 diabetes, Alzheimer's, and various types of cancer. F...