Functional coupling between DnaK and bacterial RNA polymerase through a dedicated J-domain protein system [0.03%]
通过一个特定的J域蛋白系统将DnaK与细菌RNA聚合酶功能偶联起来
Safa Boussouar,Amine Ali Chaouche,Yann Denis et al.
Safa Boussouar et al.
J-domain proteins (JDPs) are co-chaperones of Hsp70/DnaK, conferring a broad range of functions to the Hsp70/DnaK chaperone system. The shortest characterized JDP is AtcJ, which is part of an essential four-protein system dedicated to the c...
Single-Molecule Structures and Kinetics of the AAA-ATPase Bcs1 translocating folded ISP [0.03%]
单个分子水平上AAA-ATP酶Bcs1转运折叠型ISP的结构和动力学研究
Yangang Pan,Jingyu Zhan,Zhaokun Wang et al.
Yangang Pan et al.
Bcs1 is a AAA-ATPase that transports the Rieske iron-sulfur protein (ISP) across the inner mitochondrial membrane. Bcs1 is a particular molecular machine in many regards: In contrast to canonical, hexameric, soluble, AAA-ATPases (i) Bcs1 is...
Yang Yu,Jinxing Ou,Nathalie Dostatni
Yang Yu
Since its first adaptation in fruit fly embryos with the fluorescent tagging of RNA, live imaging of transcription during development revealed the unanticipated importance of the temporal dynamics in the decoding of positional information. ...
Single-molecule microscopy reveals that TFIIE subunits dynamically interact with preinitiation complexes in a manner impacted by TFIIH [0.03%]
单分子显微镜观测表明TFIIE亚基以动态方式与转录起始前复合物相互作用，并受到TFIIH的影响
Stephen R Archuleta,Ryan C Miller,Julia A Mirita et al.
Stephen R Archuleta et al.
Transcription by RNA polymerase II (Pol II) requires general transcription factors that bind with Pol II at the promoters of protein-coding genes to form preinitiation complexes (PICs). Among these is TFIIE, which recruits TFIIH to the PIC ...
Agata D Misiaszek,Jeffrey A Chao
Agata D Misiaszek
Once an mRNA is exported to the cytoplasm, a transcript's fate is determined by the combinatorial interaction of a myriad of RNA-binding proteins that will determine its localization, translation and, eventual, degradation. To understand ho...
Monomeric structure of influenza A virus NEP/NS2 obtained with an artificial protein highlights conformational plasticity [0.03%]
人工蛋白获得的流感病毒NEP/NS2单体结构揭示了构象多样性
Alice J Stelfox,Mélissa Bessonne,Jean-Marie Bourhis et al.
Alice J Stelfox et al.
The influenza virus nuclear export protein (NEP)/non-structural protein 2 (NS2) is a multifunctional protein, involved in viral ribonucleoprotein export from the nucleus, genome replication enhancement, and the adaption of avian influenza t...
RNA Structural Ensemble Determinants of -1 Programmed Ribosomal Frameshifting Efficiency Across Coronavirus Evolution [0.03%]
冠状病毒进化过程中引发-1程序化核糖体框移效率的RNA结构组合特征
Scott R Allen,Tamar Schlick,Alain Laederach
Scott R Allen
Coronavirus -1 programmed ribosomal frameshifting (-1 PRF) elements adopt complex structural ensembles that are essential for maintaining efficient frameshifting. Here we study four novel -1 PRF elements to experimentally determine and char...
EGFR activation and conformational "convergence": an emerging allosteric model from cancer mutations [0.03%]
EGFR激活和构象“趋同”：来自癌症突变的新兴别构模型
Laura Orellana
Laura Orellana
Since its isolation nearly 50 years ago, the Epidermal Growth Factor Receptor (EGFR) has become one of the most extensively studied oncogenes. However, scarce data on the full-length receptor, together with its flexibility, has left critica...
Adam M B Allen,Shannon J McKie,Christian G Noble et al.
Adam M B Allen et al.
DNA topoisomerase VI (topo VI) is a type IIB topoisomerase that was originally found in archaea but later found in plants and some other eukaryotes and certain bacteria; claims that it is present in plasmodial parasites have yet to be subst...
Biochemical and Structural Analyses of the Tardigrade DNA-Damage Suppressor Protein, Dsup [0.03%]
缓步动物DNA损伤抑制蛋白Dsup的生化和结构研究分析
Tyler J Woodward,M Todd Washington
Tyler J Woodward
Tardigrades are extremophiles that withstand harsh environments through unique molecular strategies. One such strategy involves Damage Suppressor (Dsup), a protein shown to protect cells from radiation-induced DNA damage. Little is known ab...