Classification of cell subpopulations using multiple cellular parameters from high-content imaging studies [0.03%]
基于高通量图像的多细胞参数的子群体分类方法
Shuguang Huang
Shuguang Huang
Cell samples from in vitro studies are almost never uniform; individual cells of a population may differ with respect to their developmental stage, phase in the cell cycle, state of transfection, or by natural variability. Therefore, a samp...
Expression and purification of PI3 kinase alpha and development of an ATP depletion and an alphascreen PI3 kinase activity assay [0.03%]
PI3Kα的表达、纯化及ATP亏损和AlphaScreen法测定PI3K活性的方法学开发
Brigitte Boldyreff,Tine L Rasmussen,Hans H Jensen et al.
Brigitte Boldyreff et al.
Phosphoinositide-3-kinases are important targets for drug development because many proteins in the PI3 kinase signaling pathway are mutated, hyperactivated, or overexpressed in human cancers. Here, the authors coexpressed the human class Ia...
beta-Arrestin recruitment assay for the identification of agonists of the sphingosine 1-phosphate receptor EDG1 [0.03%]
招募β-逮捕蛋白鉴定鞘氨醇-1-磷酸受体EDG1激动剂的试验
Miranda M C van Der Lee,Maaike Bras,Chris J van Koppen et al.
Miranda M C van Der Lee et al.
beta-Arrestin recruitment assays provide a generic assay platform for drug discovery on G-protein-coupled receptors (GPCRs). The PathHunter assay technology developed by DiscoveRx (Fremont, CA) uses enzyme fragment complementation of beta-g...
Mouse Bsep ATPase assay: a nonradioactive tool for assessment of the cholestatic potential of drugs [0.03%]
非放射性药物致胆汁淤积潜能评估工具:Bsep ATP酶检测方法研究进展
Emese Kis,Zsuzsanna Rajnai,Eniko Ioja et al.
Emese Kis et al.
The mouse ortholog of the human bile salt export pump (BSEP) transporter was expressed in a baculovirus-infected insect cell (Sf9) system to study the effect of membrane cholesterol content on the transporter function. The transport activit...
Khadijah Makky,Petar Duvnjak,Kallal Pramanik et al.
Khadijah Makky et al.
Regulation of whole-body metabolism and energy homeostasis has been shown to require signaling between multiple organs. To identify genetic programs that determine metabolic rate, and compounds that can modify it, a whole-animal assay amena...
Duplexed label-free G protein--coupled receptor assays for high-throughput screening [0.03%]
GPCRs高通量筛选的双通道无标记检测方法
Elizabeth Tran,Ye Fang
Elizabeth Tran
This article describes duplexed label-free optical biosensor cellular assays for simultaneously assaying 2 endogenous receptors, the G(q)-coupled histamine receptor (H( 1)) and the G(s)-coupled beta(2)-adrenergic receptor (beta(2)AR), in A4...
Rakesh Rathore,Jay Corr,George Scott et al.
Rakesh Rathore et al.
Commonly used methods for isolated enzyme inhibitor screening typically rely on fluorescent or chemiluminescent detection techniques that are often indirect and/or coupled assays. Mass spectrometry (MS) has been widely reported for measurin...
Caroline Engeloch,Ulrich Schopfer,Ingo Muckenschnabel et al.
Caroline Engeloch et al.
The impact of storage conditions on compound stability and compound solubility has been debated intensely over the past 5 years. At Novartis, the authors decided to opt for a storage concept that can be considered controversial because they...
High-content image-based screening for small-molecule chaperone amplifiers in heat shock [0.03%]
基于高内涵图像的热休克小分子伴侣放大剂筛选
Qingyan Au,Prim Kanchanastit,Jack R Barber et al.
Qingyan Au et al.
Heat shock proteins represent the major elements of the cellular stress response that protects cells from diseases caused by protein misfolding. Small-molecule amplifiers of heat shock proteins have shown promising results in several animal...
Atom- and bond-based 2D TOMOCOMD-CARDD approach and ligand-based virtual screening for the drug discovery of new tyrosinase inhibitors [0.03%]
基于原子和键的二维TOMOCOMD-CARDD方法及基于配体的虚拟筛选在酪氨酸酶抑制剂新药发现中的应用
Gerardo M Casañola-Martín,Yovani Marrero-Ponce,Mahmud Tareq Hassan Khan et al.
Gerardo M Casañola-Martín et al.
Two-dimensional atom- and bond-based TOMOCOMD-CARDD descriptors and linear discriminant analysis (LDA) are used in this report to perform a quantitative structure-activity relationship (QSAR) study of tyrosinase-inhibitory activity. A datab...