Identification of a high-affinity anti-phosphoserine antibody for the development of a homogeneous fluorescence polarization assay of protein kinase C [0.03%]
一种高亲和力的抗磷丝氨酸抗体的鉴定及其在蛋白激酶C均相荧光偏振测定中的应用研究
J J Wu,D R Yarwood,Q Pham et al.
J J Wu et al.
In the last few years, fluorescence polarization (FP) has been applied to the development of robust, homogeneous, high throughput assays in molecular recognition research, such as ligand-protein interactions. Recently, this technology has b...
Comparative Study
Journal of biomolecular screening. 2000 Feb;5(1):23-30. DOI:10.1177/108705710000500106 2000
Microbiological high throughput screening: an opportunity for the lead discovery process [0.03%]
微生物高通量筛选在先导化合物发现过程中的应用前景
M H Beydon,A Fournier,L Drugeault et al.
M H Beydon et al.
Microbial HTS has been implemented at Rhône-Poulenc Rorer through the development of a dedicated robotic platform. This robot (Turbo) has been designed with the aim of fully integrating microbial HTS into the lead discovery processes. Inno...
Test tube's end [0.03%]
试管末期
M R Knapp,S Sundberg,J W Parce
M R Knapp
J J Burbaum
J J Burbaum
M Schuster,E Wasserbauer,A Einhauer et al.
M Schuster et al.
Identification of new target proteins is a novel paradigm in drug discovery. A major bottleneck of this strategy is the rapid and simultaneous expression of proteins from differential gene expression to identify eligible candidates. By sear...
Development of high throughput screening assays using fluorescence polarization: nuclear receptor-ligand-binding and kinase/phosphatase assays [0.03%]
用于高通量筛选的以荧光偏振技术为基础的受体/配基及蛋白激酶/磷酸酶测定法的研制和发展
G J Parker,T L Law,F J Lenoch et al.
G J Parker et al.
Fluorescence polarization (FP) has been used to develop high throughput screening (HTS) assays for nuclear receptor-ligand displacement and kinase inhibition. FP is a solution-based, homogeneous technique requiring no immobilization or sepa...
Miniaturization of a functional transcription assay in yeast (human progesterone receptor) in the 384- and 1536-well plate format [0.03%]
酵母（人类孕激素受体）功能转录测定的微型化（384和1536孔板格式）
M Berg,K Undisz,R Thiericke et al.
M Berg et al.
Miniaturization of high throughput screening assays to high-density microplate formats (384 or 1536 wells) is currently the focus of research activity in modern drug discovery facilities. In this article, we describe the adaptation of a flu...
High throughput fluorescence polarization: a homogeneous alternative to radioligand binding for cell surface receptors [0.03%]
高通量荧光偏振法：细胞表面受体放射配基结合的均相替代方法
M Allen,J Reeves,G Mellor
M Allen
High throughput fluorescence polarization (FP) assays are described that offer a nonradioactive, homogeneous, and low-cost alternative to radioligand binding assays for cell surface receptors (G protein-coupled receptors and ligand-gated io...