Development of a cyanovirin-N-HIV-1 gp120 binding assay for high throughput screening of natural product extracts by time-resolved fluorescence [0.03%]
时间分辨荧光法测定链球菌凝集素与HIV-1gp120结合用于高效筛选天然产物提取物的建立
J B McMahon,J A Beutler,B R O&#x;Keefe et al.
J B McMahon et al.
The unique, high-affinity binding of cyanovirin-N (CV-N), a potent anti-human immunodeficiency virus (HIV) protein, to the HIV envelope glycoprotein gp120, was exploited to develop an HTS assay in an attempt to discover small-molecule mimet...
Fluorescence polarization assays for high throughput screening of G protein-coupled receptors [0.03%]
GPCRs高通量筛选的荧光偏振检测法
P Banks,M Gosselin,L Prystay
P Banks
Fluorescence polarization assays in 384-well microtiter plates have been demonstrated. The performance is suitable for high throughput drug screening applications with respect to speed of analysis, displaceable signal, precision, and sensit...
Development of a high throughput scintillation proximity assay for hepatitis C virus NS3 protease that reduces the proportion of competitive inhibitors identified [0.03%]
一种高通量闪烁接近法测定试剂盒的开发用于丙型肝炎病毒NS3蛋白酶测定减少竞争性抑制剂的比例
A Fowler,M Price-Jones,K Hughes et al.
A Fowler et al.
A screening assay has been developed for hepatitis C virus (HCV) NS3 protease using the scintillation proximity assay (SPA) technology. The sequence of the peptide substrate used was taken from the site cleaved by the enzyme in the mature n...
Novel fluorescent technology platform for high throughput cytotoxicity and proliferation assays [0.03%]
一种新型的用于高通量细胞毒性和增值实验的荧光技术平台
M Wodnicka,R D Guarino,J J Hemperly et al.
M Wodnicka et al.
We have developed a novel fluorescent Oxygen BioSensor technology platform adaptable to many applications in the area of drug discovery and development, particularly cell-based assays. This biosensor technology requires no additional reagen...
Large-scale screening assay to measure epidermal growth factor internalization [0.03%]
大规模筛选测定表皮生长因子内吞量的方法
R de Wit,C M Hendrix,J Boonstra et al.
R de Wit et al.
Recently, we showed that the internalization of the epidermal growth factor (EGF) receptor is inhibited by hydrogen peroxide (H(2)O(2)) in human fibroblasts. In order to test the effect of various stress conditions on receptor internalizati...
J R Lakowicz,I Gryczynski,Z Gryczynski
J R Lakowicz
We describe two new methods of fluorescence sensing for use in high throughput screening (HTS). Modulation sensing transforms analyte-dependent intensity changes into a change in the low-frequency modulation signal. Polarization sensing tra...
D Dunnington
D Dunnington
T Mander,R Turner
T Mander
Implementing multilevel dynamic scheduling for a highly flexible 5-rail high throughput screening system [0.03%]
实现五轨高通量筛选系统的多级动态调度
M N Feiglin,S Skwish,M Laab et al.
M N Feiglin et al.
As automation solves the bottleneck involved in drug screening, new bottlenecks present themselves. Some of these bottlenecks include sample management, hit picking and confirmation, and reagents lost as a result of incomplete runs. To keep...
An ultra-high throughput screening approach for an adenine transferase using fluorescence polarization [0.03%]
一种使用荧光偏振对腺嘌呤转移酶进行超高通量筛选的方法
Z Li,S Mehdi,I Patel et al.
Z Li et al.
We have developed a novel assay for measuring the activity of an enzyme that transfers multiple adenine-containing groups to an acceptor protein. The assay is based on fluorescence polarization (FP) technology in a 1536-well plate format. I...