Fluorescence polarization and anisotropy in high throughput screening: perspectives and primer [0.03%]
HTS中的荧光偏振和各向异性：透视与入门指导
J C Owicki
J C Owicki
Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to co...
Development of a 5-hydroxytryptamine(2A) receptor binding assay for high throughput screening using 96-well microfilter plates [0.03%]
一种用于高通量筛选的5-羟色胺（2A）受体结合试验的开发使用96孔微滤板
A Harms,D Gündisch,C E Müller et al.
A Harms et al.
A high throughput screening method for the analysis of 5-hydroxytryptamine(2A) (5-HT(2A)) receptor binding parameters has been developed, using 96-well filter plates of the Millipore MultiScreen system in combination with a MicroBeta PLUS m...
Comparative Study
Journal of biomolecular screening. 2000 Aug;5(4):269-78. DOI:10.1177/108705710000500410 2000
High throughput screening for human interferon-gamma production inhibitor using homogenous time-resolved fluorescence [0.03%]
利用均相时间分辨荧光进行高通量筛选人γ-干扰素生成抑制剂的研究
K Enomoto,Y Aono,T Mitsugi et al.
K Enomoto et al.
An immunoassay for interferon-gamma (IFN-gamma) using homogeneous time-resolved fluorescence (HTRF) has been developed. In this assay, IFN-gamma can be detected by simply adding a mixture of three reagents-biotinylated polyclonal antibody, ...
Comparative Study
Journal of biomolecular screening. 2000 Aug;5(4):263-8. DOI:10.1177/108705710000500409 2000
Development of a functional reporter gene HTS assay for the identification of mGluR7 modulators [0.03%]
功能性报告基因HTS测定法的开发用于识别mGluR7调节物
G C Terstappen,A Giacometti,E Ballini et al.
G C Terstappen et al.
For the identification of modulators of the metabotropic glutamate receptor mGluR7, a functional cell-based high throughput screening (HTS) assay was developed. This assay utilizes the signal transduction pathway of mGluR7, which is negativ...
A fluorescent cell-based assay for cytochrome P-450 isozyme 1A2 induction and inhibition [0.03%]
一种用于细胞色素P-450同工酶1A2诱导和抑制的荧光细胞测定法
J H Kelly,N L Sussman
J H Kelly
High throughput lead optimization requires simple, homogeneous cell-based assays capable of defining the druglike properties of first-round screening hits. Induction and inhibition of the Phase I drug-metabolizing enzymes are central to thi...
A novel high throughput chemiluminescent assay for the measurement of cellular cyclic adenosine monophosphate levels [0.03%]
一种新型的化学发光高通量细胞内环腺苷酸检测方法
A C Chiulli,K Trompeter,M Palmer
A C Chiulli
The second messenger 3', 5'-cyclic AMP (cAMP) is a highly regulated molecule that is governed by G protein-coupled receptor activation and other cellular processes. Measurement of cAMP levels in cells is widely used as an indicator of recep...
A novel and robust homogeneous fluorescence-based assay using nanoparticles for pharmaceutical screening and diagnostics [0.03%]
一种新颖且稳健的基于荧光和纳米颗粒的均相检测方法用于药物筛选和诊断
S Schaertl,F J Meyer-Almes,E Lopez-Calle et al.
S Schaertl et al.
We have established a new type of homogeneous immunoassay based on nanoparticles (nanoparticle immunoassay, or NPIA) being analyzed using fluorescence intensity distribution analysis (FIDA). This method allows the characterization of single...
Comparative Study
Journal of biomolecular screening. 2000 Aug;5(4):227-38. DOI:10.1177/108705710000500405 2000
Automated assay optimization with integrated statistics and smart robotics [0.03%]
集成了统计分析和智能机器人的自动滴定试验优化方法
P B Taylor,F P Stewart,D J Dunnington et al.
P B Taylor et al.
The transition from manual to robotic high throughput screening (HTS) in the last few years has made it feasible to screen hundreds of thousands of chemical entities against a biological target in less than a month. This rate of HTS has inc...
Comparative Study
Journal of biomolecular screening. 2000 Aug;5(4):213-26. DOI:10.1177/108705710000500404 2000
Detection of p56(lck) kinase activity using scintillation proximity assay in 384-well format and imaging proximity assay in 384- and 1536-well format [0.03%]
使用闪烁接近法（384孔板）和影像接近分析法（384孔板及1536孔板）检测p56(lck)激酶活性
M Beveridge,Y W Park,J Hermes et al.
M Beveridge et al.
p56(lck) is a lymphocyte-specific tyrosine kinase that plays an important role in both T-cell maturation and activation. We have developed a homogeneous assay in which p56(lck) catalyzes the transfer of the gamma-phosphate group from [gamma...
Comparative Study
Journal of biomolecular screening. 2000 Aug;5(4):205-12. DOI:10.1177/108705710000500403 2000
Ultra-high throughput screen of two-million-member combinatorial compound collection in a miniaturized, 1536-well assay format [0.03%]
一百五十万种化合物文库在微型化、一万五千三百八十进制测定模式中的极高通量筛选
D Dunn,M Orlowski,P McCoy et al.
D Dunn et al.
Results of a complete survey of the more than 2-million-member Pharmacopeia compound collection in a 1536-well microvolume screening assay format are reported. A complete technology platform, enabling the performance of ultra-high throughpu...