Development of a homogeneous high throughput fluorescence polarization assay for G protein-coupled receptor binding [0.03%]
均一高通量荧光偏振检测技术在G蛋白偶联受体结合试验中的应用和发展
P H Lee,D J Bevis
P H Lee
Traditional methods that follow receptor ligand interactions are competitive assays in which the test compound displaces a radiolabeled molecule. These assays require either a time-consuming step for separation of free ligands from bound li...
Development of a facile method for high throughput screening with reporter gene assays [0.03%]
简易高效筛选报告基因的检测方法及其应用
A S Goetz,J L Andrews,T R Littleton et al.
A S Goetz et al.
This report describes a facile methodology for high throughput screening with stable mammalian cell reporter gene assays. We have adapted a 96-well adherent cell method to an assay in which cells propagated in suspension are dispensed into ...
Evaluation of electrochemiluminescent technology for inhibitors of granulocyte colony-stimulating factor receptor binding [0.03%]
人粒细胞集落刺激因子受体结合抑制剂的电化学发光技术评价
S M Gopalakrishnan,U Warrior,D Burns et al.
S M Gopalakrishnan et al.
An electrochemiluminescent (ECL) assay was developed to identify compounds that inhibit the interaction of granulocyte colony-stimulating factor (GCSF) with its recombinant human receptor. The ECL technology uses a tris-(bipyridine) chelate...
Comparative Study
Journal of biomolecular screening. 2000 Oct;5(5):369-76. DOI:10.1177/108705710000500509 2000
High-capacity screening of arylalkylamine N-acetyltransferase inhibitors using a high-performance liquid chromatography system [0.03%]
采用高效液相色谱系统筛选高通量的芳基烷胺N-乙酰转移酶抑制剂
G Ferry,J A Boutin
G Ferry
Systematic screening is a natural development of any pharmacological program. Most enzyme inhibitor screens use indirect or "aspecific" methods, such as colorimetric or fluorimetric ones. These screening methods cause quite a few false-posi...
Characterization of a set of HIV-1 protease inhibitors using binding kinetics data from a biosensor-based screen [0.03%]
利用生物传感器筛选结合动力学数据表征一系列HIV-1蛋白酶抑制剂
M D Hämäläinen,P O Markgren,W Schaal et al.
M D Hämäläinen et al.
The interaction between 290 structurally diverse human immunodeficiency virus type 1 (HIV-1) protease inhibitors and the immobilized enzyme was analyzed with an optical biosensor. Although only a single concentration of inhibitor was used, ...
Application of QuantiGene nucleic acid quantification technology for high throughput screening [0.03%]
Quantigene核酸定量技术在高通量药物筛选中的应用研究
U Warrior,Y Fan,C A David et al.
U Warrior et al.
To identify inhibitors of interleukin-8 (IL-8) production, a high throughput assay was developed using the QuantiGene nucleic acid quantification kit that employs branched-chain DNA (bDNA) technology to measure the mRNA directly from cells....
Pattern recognition analysis of endogenous cell metabolites for high throughput mode of action identification: removing the postscreening dilemma associated with whole-organism high throughput screening [0.03%]
高效识别作用模式的内源细胞代谢物模式识别分析：消除与整体高通量筛选相关的确证筛查两难问题
S J Hole,P W Howe,P D Stanley et al.
S J Hole et al.
Although whole-organism HTS can give clear indications of in vivo activity, typically few clues are given as to the mechanism of action (MOA), and determining the MOA for large numbers of active compounds can be costly and complex-an altern...
Impact of a red-shifted dye label for high throughput fluorescence polarization assays of G protein-coupled receptors [0.03%]
一种红偏移染料标记对G蛋白偶联受体的高通量荧光极化测定的影响研究
P Banks,M Gosselin,L Prystay
P Banks
High throughput screening fluorescence polarization assays using G protein-coupled receptors (GPCRs) as targets have been compared using fluorescein and BODIPY TMR-labeled peptides. The red-shifted BODIPY TMR dye exhibits improved assay per...
Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer [0.03%]
基于荧光共振能量转移的泛素转移反应测定法的建立及高通量筛选应用研究
M D Boisclair,C McClure,S Josiah et al.
M D Boisclair et al.
An assay based on fluorescence resonance energy transfer (FRET) has been developed to screen for ubiquitination inhibitors. The assay measures the transfer of ubiquitin from Ubc4 to HECT protein Rsc 1083. Secondary reagents (streptavidin an...
Development and application of a GFP-FRET intracellular caspase assay for drug screening [0.03%]
药物筛选用的GFP-FRET细胞内半胱天冬酶检测试剂盒的研发及应用
J Jones,R Heim,E Hare et al.
J Jones et al.
Apoptosis is a crucial biological process, and activation of caspase endoproteases is essential for proper regulation and execution of apoptosis. Because caspases also appear to be central players in several pathological states, there is a ...