Development of SPC-ELISA: a new assay principle for the study of sulfated polysaccharide-protein interactions [0.03%]
新型SPC-ELISA测定法的研究及糖蛋白之间相互作用的作用机制研究
S Alban,R Gastpar
S Alban
A sulfated polysaccharide-coating enzyme-linked immunosorbent assay (SPC-ELISA), a new screening assay for the study of interactions between sulfated polysaccharides and proteins, has been developed. Fibrinogen was used as representative fo...
High throughput cell-based assay of hematopoietic progenitor differentiation [0.03%]
高通量的细胞基质分化测定法在造血干细胞中的应用研究
D E Greenwalt,J Szabo,I Manchel
D E Greenwalt
The in vitro efficacy of drug candidates relative to hematopoietic stem cell proliferation and differentiation is currently assayed through use of the clonogenic "colony assay." The extremely low throughput of this assay precludes its use i...
C Giordano
C Giordano
A simple automated solution for removing and applying sealing microplate lids [0.03%]
一种简单的自动化解决方案，用于移除和安装密封微孔板盖子
G A Clarke,M N Feiglin,G W King et al.
G A Clarke et al.
With the improved reliability and efficiency of automation, there has been an increased desire to integrate automated sample management with automated screening systems. In order to store samples "on line" for an extended period of time, an...
Development and evaluation of high throughput functional assay methods for HERG potassium channel [0.03%]
高效的功能检测方法的开发和评价用于HERG钾离子通道
W Tang,J Kang,X Wu et al.
W Tang et al.
Three functional hERG channel assay methods have been developed and evaluated. The methods were tested against five known hERG channel inhibitors: dofetilide, terfenadine (Seldane), sertindole (Serdolect), astemizole (Hismanal), and cisapri...
Comparative Study
Journal of biomolecular screening. 2001 Oct;6(5):325-31. DOI:10.1177/108705710100600506 2001
M E Schurdak,M J Voorbach,L Gao et al.
M E Schurdak et al.
Gel permeation methods have been commonly used to screen combinatorial libraries synthesized on a solid support. We report here three screens of combinatorial libraries using gel permeation assays. These include a simple enzymatic assay to ...
Validation of FLIPR membrane potential dye for high throughput screening of potassium channel modulators [0.03%]
FLIPR膜电位染料验证钾通道调节剂高通量筛选的有效性研究
K L Whiteaker,S M Gopalakrishnan,D Groebe et al.
K L Whiteaker et al.
A fluorescence-based assay using the FLIPR Membrane Potential Assay Kit (FMP) was evaluated for functional characterization and high throughput screening (HTS) of potassium channel (ATP-sensitive K+ channel; K(ATP)) modulators. The FMP dye ...
High resolution screening of plant natural product extracts for estrogen receptor alpha and beta binding activity using an online HPLC-MS biochemical detection system [0.03%]
基于在线HPLC-MS生化检测系统的高分辨率筛选植物天然产物提取物的雌激素受体α和β结合活性
U Schobel,M Frenay,D A van Elswijk et al.
U Schobel et al.
A new screening technology that combines biochemical analysis with the resolution power of high-performance liquid chromatography (HPLC), referred to here as high-resolution screening (HRS) technique, is described. The capability of the HRS...
Real experiences of uHTS: a prototypic 1536-well fluorescence anisotropy-based uHTS screen and application of well-level quality control procedures [0.03%]
uHTS的实际经验：基于荧光偏振的1536孔板的uHTS筛选及应用单孔水平质量控制方法
S Turconi,K Shea,S Ashman et al.
S Turconi et al.
This paper describes, for the first time, a true ultra-high throughput screen (uHTS) based upon fluorescence anisotropy and performed entirely in 1536-well assay plates. The assay is based upon binding and displacement of a BODIPY-FL-labele...
A cGMP-dependent protein kinase assay for high throughput screening based on time-resolved fluorescence resonance energy transfer [0.03%]
一种基于时间分辨荧光共振能量转移的cGMP依赖型蛋白激酶检测方法及高通量筛选中的应用研究
B Bader,E Butt,A Palmetshofer et al.
B Bader et al.
Activation of cyclic GMP-dependent protein kinase (cGK) is an important event in the regulation of blood pressure and platelet function. Upstream signals are the generation of nitric oxide (NO) by NO synthases and the subsequent rise in cyc...