The effect of ICER on screening methods involving CRE-mediated reporter gene expression [0.03%]
ICER对涉及CRE介导的报告基因表达的筛选方法的影响
Daniel M Kemp,Samantha E George,Toby C Kent et al.
Daniel M Kemp et al.
We describe a mechanism whereby increasing levels of cAMP in Chinese hamster ovary (CHO) and other cell lines lead to a significant repression in cAMP response element (CRE)-mediated luciferase reporter gene expression. This effect was show...
A colorimetric assay to quantify dehydrogenase activity in crude cell lysates [0.03%]
一种测定细胞裂解物中脱氢酶活性的比色法
Kimberly M Mayer,Frances H Arnold
Kimberly M Mayer
Nitroblue tetrazolium (NBT) in the presence of phenazine methosulfate (PMS) reacts with the NADPH produced by dehydrogenases to produce an insoluble blue-purple formazan. Endpoint assays taking advantage of this reaction have been successfu...
A luminescent Escherichia coli biosensor for the high throughput detection of beta-lactams [0.03%]
一种用于高通量检测β-内酰胺类的发光大肠杆菌生物传感器
Satu J Valtonen,Jussi S Kurittu,Matti T Karp
Satu J Valtonen
A group-specific bioluminescent Escherichia coli strain for studying the action of beta-lactam antibiotics is described. The strain contains a plasmid, pBlaLux1, in which the luciferase genes from Photorhabdus luminescens are inserted under...
An Escherichia coli biosensor strain for amplified and high throughput detection of antimicrobial agents [0.03%]
一种用于放大和高通量检测抗菌剂的大肠杆菌生物传感器菌株
Minna-Liisa Ankö,Jussi Kurittu,Matti Karp
Minna-Liisa Ankö
We report here the construction of a bacterial reporter system for high-throughput screening of antimicrobial agents. The test organism is the Escherichia coli K-12 strain carrying luciferase genes luxC, luxD, luxA, luxB, and luxE from the ...
Considerations for using fluorescence polarization in the screening of G protein-coupled receptors [0.03%]
G蛋白偶联受体筛选中荧光偏振法的使用及其考虑因素
Peter Banks,Michael Harvey
Peter Banks
Fluorescence polarization must be classified as a unique detection method relative to other intensity-based methods because both bound and unbound tracer is measured. Other fluorescence techniques require either physical removal of unbound ...
High throughput screening for cyanovirin-N mimetics binding to HIV-1 gp41 [0.03%]
用于筛选HIV-1gp41与拟似物结合的高通量药物筛选模型的研究
John A Beutler,James B McMahon,Tanya R Johnson et al.
John A Beutler et al.
The human immunodeficiency virus type-1 (HIV-1) envelope glycoprotein gp41 is an important mediator of viral entry into host cells. Previous studies showed that the virucidal protein cyanovirin-N (CV-N) bound to both gp120 and gp41, and tha...
An effective tool for homogeneous assays using a simple robot arm with Stackers-TRISTAN [0.03%]
一种有效均相检测方法及其采用的简单机械手Stacker-TRISTAN
Kazuyoshi Yajima,Takanori Ohgaru,Yoshifumi Hashimoto et al.
Kazuyoshi Yajima et al.
The Twister & RapidPlate Integrated System by TANabe, or TRISTAN, consists of a 96-channel dispenser (RapidPlate 96), a plate reader (V-MAX), and a simple robot arm (Twister). We developed TRISTAN for effectively conducting a homogeneous as...
A novel membrane potential-sensitive fluorescent dye improves cell-based assays for ion channels [0.03%]
一种新型膜电位敏感荧光染料可改善离子通道的细胞检测法
Deborah F Baxter,Martin Kirk,Amy F Garcia et al.
Deborah F Baxter et al.
The study of ion channel-mediated changes in membrane potential using the conventional bisoxonol fluorescent dye DiBAC(4)(3) has several limitations, including a slow onset of response and multistep preparation, that limit both the fidelity...
Comparison of high throughput screening technologies for luminescence cell-based reporter screens [0.03%]
高通量筛选技术在发光细胞基报告因子筛选中的比较研究
Gregory F Davis,Thomas R Downs,Julie A Farmer et al.
Gregory F Davis et al.
As higher density formats become more and more common in HTS labs, the expectations for maintaining faster, lower cost screens puts great pressure on traditional 96-well screens. In some cases higher density formats are not compatible with ...
Comparative Study
Journal of biomolecular screening. 2002 Feb;7(1):67-77. DOI:10.1177/108705710200700109 2002
Emmanuel Le Poul,Sunao Hisada,Yoshinori Mizuguchi et al.
Emmanuel Le Poul et al.
AequoScreen, a cellular aequorin-based functional assay, has been optimized for luminescent high-throughput screening (HTS) of G protein-coupled receptor (GPCRs). AequoScreen is a homogeneous assay in which the cells are loaded with the apo...