An automated system for screening retroviral expression constructs in microplate format [0.03%]
一种在微孔板中筛选逆转录病毒表达构建体的自动化系统
Mark D Stump,Leonardo Di Sera,Matthew Rebentisch et al.
Mark D Stump et al.
Retroviruses are useful for genetics studies to deliver genes that express proteins, peptides, and RNAs. Several steps, including DNA preparation, transfection, packaging, transduction, and assay, are required to execute screens using retro...
M Préaudat,J Ouled-Diaf,B Alpha-Bazin et al.
M Préaudat et al.
Caspases are cysteine proteases presenting a conserved active site that cleaves protein substrates at a highly specific position. They are involved in different aspects of the active cell death pathway. Most of them act through proteolytic ...
Microarray compound screening (microARCS) to identify inhibitors of HIV integrase [0.03%]
微阵列化合物筛选（微孔板ARCS）鉴定HIV整合酶抑制剂
Caroline A David,Tim Middleton,Debra Montgomery et al.
Caroline A David et al.
A novel high-throughput strand transfer assay has been developed, using Microarray Compound Screening (microARCS) technology, to identify inhibitors of human immunodeficiency virus (HIV) integrase. This technology utilizes agarose matrices ...
Victor Barsky,Alexander Perov,Sergei Tokalov et al.
Victor Barsky et al.
A series of biochip readers developed for gel-based biochips includes three imaging models and a novel nonimaging biochip scanner. The imaging readers, ranging from a research-grade versatile reader to a simple portable one, use wide-field ...
Functional screening of G protein-coupled receptors by measuring intracellular calcium with a fluorescence microplate reader [0.03%]
应用荧光酶标仪检测内钙量变化进行功能性G蛋白偶联受体筛选
Matthias U Kassack,Barbara Höfgen,Jochen Lehmann et al.
Matthias U Kassack et al.
Ligand binding studies reveal information about affinity to G protein-coupled receptors (GPCRs) rather than functional properties. Increase in intracellular Ca(2+) appears to represent a universal second messenger signal for a majority of r...
Homogeneous time-resolved fluorescence quenching assay (LANCE) for caspase-3 [0.03%]
homogeneous时间Resolved Fluorescence淬灭法（LANCE）检测caspase-3
Jarkko Karvinen,Pertti Hurskainen,Sujatha Gopalakrishnan et al.
Jarkko Karvinen et al.
In addition to kinases and G protein-coupled receptors, proteases are one of the main targets in modern drug discovery. Caspases and viral proteases, for instance, are potential targets for new drugs. To satisfy the current need for fast an...
A fluorescence polarization assay for cyclic nucleotide phosphodiesterases [0.03%]
用于环核苷酸磷酸二酯酶的荧光偏振测定法
Wei Huang,Yan Zhang,J Richard Sportsman
Wei Huang
Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of the 3'-ester bond of cyclic AMP (cAMP) and cyclic GMP (cGMP), important second messengers in the transduction of a variety of extracellular signals. There is growing int...
Comparison of assay technologies for a tyrosine kinase assay generates different results in high throughput screening [0.03%]
用于酪氨酸激酶检测的高通量筛选生成不同的分析技术结果比较
Matthew A Sills,Donna Weiss,Quynhchi Pham et al.
Matthew A Sills et al.
In today's high-throughput screening (HTS) environment, an increasing number of assay detection technologies are routinely utilized in lead finding programs. Because of the relatively broad applicability of several of these technologies, on...
Comparative Study
Journal of biomolecular screening. 2002 Jun;7(3):191-214. DOI:10.1177/108705710200700304 2002
Paul Fogel,Pascal Collette,Alain Dupront et al.
Paul Fogel et al.
HTS data from primary screening are usually analyzed by setting a cutoff for activity, in order to minimize both false-negative and false-positive rates. An alternative approach, based on a calculated probability of being active, is present...
A collaborative screening program for the discovery of inhibitors of HCV NS2/3 cis-cleaving protease activity [0.03%]
丙型肝炎病毒NS2/3裂解酶抑制剂的筛选项目
Mike Whitney,Jeffrey H Stack,Paul L Darke et al.
Mike Whitney et al.
This report describes the development of a cell-based assay for high-throughput screening and detection of small-molecule inhibitors for hepatitis C virus (HCV) NS2/3 protease. The HCV NS2/3 protease is essential for the normal infectious c...