Microplate screening for apoptosis with antibody to single-stranded DNA distinguishes anticancer drugs from toxic chemicals [0.03%]
利用单链DNA抗体筛选微板鉴定抗癌药物和有毒化学物的区别
Oskar S Frankfurt,Awtar Krishan
Oskar S Frankfurt
The effect of anticancer drugs and toxic compounds on cultures of human leukemic cells was evaluated by an enzyme-linked immunosorbent assay (Apoptosis ELISA) that uses a monoclonal antibody against single-stranded DNA to quantitate the apo...
Evaluation of fluorescent compound interference in 4 fluorescence polarization assays: 2 kinases, 1 protease, and 1 phosphatase [0.03%]
四种荧光偏振分析法中荧光化合物干扰情况的评价：两种激酶、一种蛋白酶和一种磷酸酶
Tammy C Turek-Etienne,Eliza C Small,Sharon C Soh et al.
Tammy C Turek-Etienne et al.
With the increasing use of fluorescence-based assays in high-throughput screening (HTS), the possibility of interference by fluorescent compounds needs to be considered. To investigate compound interference, a well-defined sample set of bio...
A homogeneous fluorescence polarization assay adaptable for a range of protein serine/threonine and tyrosine kinases [0.03%]
一种适于多种蛋白丝/苏氨酸和酪氨酸激酶的均相荧光偏振检测法
Elizabeth A Gaudet,Kuo-Sen Huang,Yan Zhang et al.
Elizabeth A Gaudet et al.
Recently, a new technology for high-throughput screening has been developed, called IMAP(patent pending). IMAP technology has previously been implemented in an assay for cyclic nucleotide phosphodiesterases (PDE). The authors describe the d...
Development and use of a high-throughput bacterial DNA gyrase assay to identify mammalian topoisomerase II inhibitors with whole-cell anticancer activity [0.03%]
开发和应用一种高效的大肠杆菌DNA回旋酶测定方法，以鉴定具有全细胞抗癌活性的哺乳动物拓扑异构酶II抑制剂
Siddhartha Roychoudhury,Kelly M Makin,Tracy L Twinem et al.
Siddhartha Roychoudhury et al.
A high-throughput screen (HTS) was developed and used to identify inhibitors of bacterial DNA gyrase. Among the validated hits were 53 compounds that also inhibited mammalian topoisomerase II with IC(50) values of 50% for all 3 cell lines t...
Development of a high-throughput screening assay for inhibitors of aggrecan cleavage using luminescent oxygen channeling (AlphaScreen ) [0.03%]
用于抑制软骨糖蛋白聚糖降解的高通量筛选分析的方法的研究——发光氧通道(AlphaScreen)
Jane Peppard,Fraser Glickman,Yang He et al.
Jane Peppard et al.
Aggrecan is one of the most important structural components of joint cartilage, and members of the metalloprotease (MMP) and ADAM (a disintegrin and metalloproteinase) protease families have been shown to degrade aggrecan in vivo. A robust ...
Development of a new approach for screening combinatorial libraries using MALDI-TOF-MS and HPLC-ESI-MS/MS [0.03%]
一种新的使用基质辅助激光解析离子化飞行时间质谱和高效液相色谱-电喷雾串联质谱筛选组合库的方法的研究与发展
Sonal Mathur,Marc Hassel,Frank Steiner et al.
Sonal Mathur et al.
Mass spectrometric techniques play a prominent role in the rapidly expanding field of high-throughput screening (HTS). In this paper, the authors present a novel qualitative approach for the screening of a small library of compounds using M...
High-content profiling of drug-drug interactions: cellular targets involved in the modulation of microtubule drug action by the antifungal ketoconazole [0.03%]
高内涵筛选药物相互作用：抗真菌药酮康唑调节微管靶点的细胞机制研究
Kenneth A Giuliano
Kenneth A Giuliano
Drug-drug interactions play an important role in the discovery and development of therapeutic agents. High-content profiling was developed to unravel the complexity of these interactions by providing multiparameter measurements of target ac...
Mary Catherine Dinunzio,Amy E Wilson,Steven B Pokotilow
Mary Catherine Dinunzio
Development of a high throughput screening assay for mitochondrial membrane potential in living cells [0.03%]
一种检测活细胞中线粒体膜电位的高通量筛选方法的研发
Shu-Gui Huang
Shu-Gui Huang
The mitochondrion plays a pivotal role in energy metabolism in eukaryotic cells. The electrochemical potential across the mitochondrial inner membrane is regulated to cope with cellular energy needs and thus reflects the bioenergetic state ...
Optimizing higher throughput methods to assess drug-drug interactions for CYP1A2, CYP2C9, CYP2C19, CYP2D6, rCYP2D6, and CYP3A4 in vitro using a single point IC(50) [0.03%]
优化体外药物相互作用高通量筛选方法用于评估CYP1A2、CYP2C9、CYP2C19、CYP2D6、rCYP2D6和CYP3A4酶抑制率
Feng Gao,Diane L Johnson,Sean Ekins et al.
Feng Gao et al.
Drug-drug interactions involving cytochrome P(450) (CYP) are an important factor in whether a new chemical entity will survive through to the development stage. Therefore, the identification of this potential as early as possible in vitro c...