A novel high-throughput screening assay for sickle cell disease drug discovery [0.03%]
一种新型的镰状细胞病药物筛选方法
Eszter Pais,John S Cambridge,Cage S Johnson et al.
Eszter Pais et al.
Although the pathophysiology and molecular basis of sickle cell disease (SCD) were described more than half a century ago, an effective and safe therapy is not yet available. This may be explained by the lack of a suitable high-throughput t...
Identification of small-molecule modulators of mouse SVZ progenitor cell proliferation and differentiation through high-throughput screening [0.03%]
通过高通量筛选鉴定调节小鼠SVZ干细胞增殖和分化的small-molecule调制剂
Yaping Liu,Raul Lacson,Jason Cassaday et al.
Yaping Liu et al.
Adult mouse subventricular zone (SVZ) neural stem/progenitor cells are multipotent self-renewing cells that retain the capacity to generate the major cell types of the central nervous system in vitro and in vivo. The relative ease of expand...
Automated panning and screening procedure on microplates for antibody generation from phage display libraries [0.03%]
用于从噬菌体展示文库生成抗体的微孔板自动扫描和筛选程序
Laura Turunen,Kristiina Takkinen,Hans Söderlund et al.
Laura Turunen et al.
Antibody phage display technology is well established and widely used for selecting specific antibodies against desired targets. Using conventional manual methods, it is laborious to perform multiple selections with different antigens simul...
An AlphaScreen-based high-throughput screen to identify inhibitors of Hsp90-cochaperone interaction [0.03%]
一种基于AlphaScreen的高通量筛选技术 用于识别Hsp90-cochaperone相互作用抑制剂
Fang Yi,Pingjun Zhu,Noel Southall et al.
Fang Yi et al.
Hsp90 has emerged as an important anticancer drug target because of its essential role in promoting the folding and maturation of many oncogenic proteins. The authors describe the development of the first high-throughput screen, based on Al...
Xiaohua Douglas Zhang,Shane D Marine,Marc Ferrer
Xiaohua Douglas Zhang
For hit selection in genome-scale RNAi research, we do not want to miss small interfering RNAs (siRNAs) with large effects; meanwhile, we do not want to include siRNAs with small or no effects in the list of selected hits. There is a strong...
Evaluating cellular impedance assays for detection of GPCR pleiotropic signaling and functional selectivity [0.03%]
评估GPCR多效信号和功能选择性检测的细胞阻抗测定法
Matthew F Peters,Clay W Scott
Matthew F Peters
G-protein-coupled receptors can couple to different signal transduction pathways in different cell types (termed cell-specific signaling) and can activate different signaling pathways depending on the receptor conformation(s) stabilized by ...
Gene expression profiling of functional murine embryonic stem cell-derived cardiomyocytes and comparison with adult heart: profiling of murine ESC-derived cardiomyocytes [0.03%]
小鼠胚胎干细胞源性心肌细胞的功能基因表达谱及其与成年心脏的比较
Tadahiro Shinozawa,Akiko Tsuji,Kenichi Imahashi et al.
Tadahiro Shinozawa et al.
Although embryonic stem cell (ESC)-derived cardiomyocytes may be a powerful tool in drug discovery, their potential has not yet been fully explored. Nor has a detailed comparison with adult heart tissue been performed. We have developed a m...
SCAN--a high-throughput assay for detecting small molecule binding to RNA targets [0.03%]
一种检测小分子与RNA靶点结合的高通量筛选方法(SCAN)
Chris Baugh,Shaohui Wang,Bin Li et al.
Chris Baugh et al.
A novel optical-based high-throughput screening technology has been developed for increasing the rate of discovering chemical leads against RNA targets. SCAN ( Screen for Compounds with Affinity for Nucleic Acids) is an affinity-based assay...
Monitoring protein kinase activity in cell lysates using a high-density peptide microarray [0.03%]
采用高密度肽芯片检测细胞裂解物中的蛋白激酶活性
Xiaoming Han,Go Yamanouchi,Takeshi Mori et al.
Xiaoming Han et al.
Monitoring and targeting protein kinases is widely accepted as a promising approach for disease diagnosis and drug discovery. For this purpose, the authors have developed an original type of peptide array as a high-throughput screening assa...
HA-pseudotyped retroviral vectors for influenza antagonist screening [0.03%]
假型逆转录病毒载体在流感拮抗剂筛选中的应用研究
Shi-Yun Wang,Ching-Yao Su,Mengi Lin et al.
Shi-Yun Wang et al.
Influenza infections are initiated by the binding of the influenza hemagglutinin (HA) and the cellular receptor sialic acids. The binding is followed by internalization, endocytosis, and uncoating to release the influenza genome to the cyto...