Efficient and effective compound management to support lead optimization [0.03%]
高效且有效的化合物管理以支持先导物优化
Chad W Johnson,Moneesh Chatterjee,Steve Kubala et al.
Chad W Johnson et al.
The introduction of lean thinking and Six Sigma methodologies into the drug discovery process has become an important approach for ensuring efficient workflows while containing costs. For the compound management department at Bristol-Myers ...
Best practices in compound management for preserving compound integrity and accurately providing samples for assays [0.03%]
化合物管理的最佳实践：保持化合物的完整性并准确提供用于分析的样品
Sandra L Matson,Moneesh Chatterjee,David A Stock et al.
Sandra L Matson et al.
Preserving the integrity of the compound collection and providing high-quality materials for drug discovery in an efficient and cost-effective manner are 2 major challenges faced by compound management (CM) at Bristol-Myers Squibb (BMS). Th...
COMDECOM: predicting the lifetime of screening compounds in DMSO solution [0.03%]
COMDECOM：预测药物筛选化合物在DMSO溶液中的半衰期
Emrin Zitha-Bovens,Peter Maas,Dick Wife et al.
Emrin Zitha-Bovens et al.
The technological evolution of the 1990s in both combinatorial chemistry and high-throughput screening created the demand for rapid access to the compound deck to support the screening process. The common strategy within the pharmaceutical ...
Innovation leading the way: application of lean manufacturing to sample management [0.03%]
创新引领未来：精益制造在样本管理中的应用
M Allen,M J Wigglesworth
M Allen
Historically, sample management successfully focused on providing compound quality and tracking distribution within a diverse geographic. However, if a competitive advantage is to be delivered in a changing environment of outsourcing, effic...
Detection of intracellular granularity induction in prostate cancer cell lines by small molecules using the HyperCyt high-throughput flow cytometry system [0.03%]
应用HyperCyt高通量流式细胞仪系统筛选前列腺癌细胞系中诱导胞质颗粒化的化合物
Mark K Haynes,J Jacob Strouse,Anna Waller et al.
Mark K Haynes et al.
Prostate cancer is a leading cause of death among men due to the limited number of treatment strategies available for advanced disease. Discovery of effective chemotherapeutics involves the identification of agents that inhibit cancer cell ...
Zoe Blaxill,Sue Holland-Crimmin,Rob Lifely
Zoe Blaxill
It is common knowledge in the pharmaceutical industry that the quality of a company's compound collection has a major influence on the success of biological screening in drug discovery programs. DMSO is the widely accepted solvent of choice...
Efficient elimination of nonstoichiometric enzyme inhibitors from HTS hit lists [0.03%]
从高通量筛选 hits 列表中有效剔除非计量比酶抑制剂的方法
Michael Habig,Anke Blechschmidt,Sigmar Dressler et al.
Michael Habig et al.
High-throughput screening often identifies not only specific, stoichiometrically binding inhibitors but also undesired compounds that unspecifically interfere with the targeted activity by nonstoichiometrically binding, unfolding, and/or in...
Use of reduced temperature cell pausing to enhance flexibility of cell-based assays [0.03%]
利用降低温度的细胞暂停以增强基于细胞检测的灵活性
Helen Wise,Paul William Abel,Darren Cawkill
Helen Wise
Construction and supply of cell-based reagents for in vitro plate-based screens are often highlighted as a bottleneck within drug discovery. Recent years have seen the successful application of both cryopreservation and automation to increa...
Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands [0.03%]
用于检测小分子配体的疟原虫蛋白热熔分析缓冲液优化
Gregory J Crowther,Alberto J Napuli,Andrew P Thomas et al.
Gregory J Crowther et al.
In the past decade, thermal melt/thermal shift assays have become a common tool for identifying ligands and other factors that stabilize specific proteins. Increased stability is indicated by an increase in the protein's melting temperature...
Hans Eriksson,Johan Brengdahl,Petter Sandström et al.
Hans Eriksson et al.
Assay-ready compound plates (ARPs) are sealed assay plates that contain DMSO solutions of screening compounds predispensed for particular assays. Assays are started by adding assay buffer and reagents to the ARPs. This concept offers import...