Primary cultures of embryonic chicken neurons for sensitive cell-based assay of botulinum neurotoxin: implications for therapeutic discovery [0.03%]
用于检测肉毒杆菌素的鸡胚胎神经元原代培养细胞基底测定及其在药物发现中的应用
Andrea M Stahl,Gordon Ruthel,Edna Torres-Melendez et al.
Andrea M Stahl et al.
Botulinum toxin is an exceedingly potent inhibitor of neurotransmission across the neuromuscular junction, causing flaccid paralysis and death. The potential for misuse of this deadly poison as a bioweapon has added a greater urgency to the...
Panning and identification of a colon tumor binding peptide from a phage display peptide library [0.03%]
结肠肿瘤靶向肽的筛选及鉴定
Yangde Zhang,Jiji Chen,Yanqiong Zhang et al.
Yangde Zhang et al.
Tumor-targeting therapy can be an efficacious way to cure a malignant tumor in clinical trials. Phage display is a molecular diversity technology that allows the presentation of a large number of peptides or proteins on the surface of filam...
Claudia C Quentmeier,Axel Wehling,Peter J Walla
Claudia C Quentmeier
The capability of using ultrafast detection technologies for a fast analysis of biomolecular reactions has been explored. As an example, the ultrafast response of tetramethylrhodamine (TMR)-labeled bovine serum albumin (BSA) as a function o...
Evaluation of cellular dielectric spectroscopy, a whole-cell, label-free technology for drug discovery on Gi-coupled GPCRs [0.03%]
评估用于Gi偶联G蛋白耦合受体药物发现的全细胞无标记技术 cellular dielectric spectroscopy 的效果
Matthew F Peters,Katharine S Knappenberger,Deidre Wilkins et al.
Matthew F Peters et al.
Cellular dielectric spectroscopy (CDS) is an emerging technology capable of detecting a range of whole-cell responses in a label-free manner. A new CDS-based instrument, CellKey, has been developed that is optimized for G-protein coupled re...
Identifying nonselective hits from a homogeneous calcium assay screen [0.03%]
基于均相钙释放试验的非特异性抑制剂筛选模型及应用
Kelly J Cassutt,Michael J Orsini,Mojgan Abousleiman et al.
Kelly J Cassutt et al.
The authors used a homogeneous calcium dye kit with a cell line transfected using a recombinant protein construct to screen a 50,000 compound library for G-protein coupled receptor (GPCR) agonists. Only 1 of the 365 primary hits activated G...
Comparative Study
Journal of biomolecular screening. 2007 Mar;12(2):285-7. DOI:10.1177/1087057106298538 2007
Using ligand-induced conformational change to screen for compounds targeting G-protein-coupled receptors [0.03%]
利用配体诱发的构象变化筛选G蛋白偶联受体靶向化合物
Brian F O&#x;Dowd,Mohammad Alijaniaram,Xiaodong Ji et al.
Brian F O&#x;Dowd et al.
The authors describe a novel drug strategy designed as a primary screen to discover either antagonist or agonist compounds targeting G-protein-coupled receptors (GPCRs). The incorporation of a nuclear localization sequence (NLS, a 5 amino a...
A simple strategy for mitigating the effect of data variability on the identification of active chemotypes from high-throughput screening data [0.03%]
一种简便策略：减轻数据变异性对高通量筛选数据中活性化学基因型鉴定的影响
Stephen R Johnson,Ramesh Padmanabha,Wayne Vaccaro et al.
Stephen R Johnson et al.
Among the several goals of a high-throughput screening campaign is the identification of as many active chemotypes as possible for further evaluation. Often, however, the number of concentration response curves (e.g., IC(50)s or K(i)s) that...
Comparative Study
Journal of biomolecular screening. 2007 Mar;12(2):276-84. DOI:10.1177/1087057106297826 2007
A luminescent oxygen channeling immunoassay for the determination of insulin in human plasma [0.03%]
一种用于测定人血浆中胰岛素的发光氧传递免疫测定法
Fritz Poulsen,Kirsten Borres Jensen
Fritz Poulsen
The authors describe a homogeneous, sensitive, and rapid bead-based sandwich immunoassay with a broad analytical range for quantifying insulin in human plasma. The assay was performed as a 2-step reaction by incubating the sample with a mix...
Comparative Study
Journal of biomolecular screening. 2007 Mar;12(2):240-7. DOI:10.1177/1087057106297566 2007
Turbo-mixing in microplates [0.03%]
微孔板中的涡混合法
Elisa Mitre,Margarete Schulze,Gerhard A Cumme et al.
Elisa Mitre et al.
How to effectively mix small volumes of liquids within microplate wells is a still underestimated and often neglected challenge. The method the authors introduce here relies on violent turbulent motion within a liquid caused by spotting an ...
Identification of novel human high-density lipoprotein receptor Up-regulators using a cell-based high-throughput screening assay [0.03%]
基于细胞的高通量筛选鉴定新型人类高密度脂蛋白受体表达诱导化合物
Yuan Yang,Zhongbing Zhang,Wei Jiang et al.
Yuan Yang et al.
Scavenger receptor class B type I (SR-BI) is the high-affinity high-density lipoprotein (HDL) receptor, and CLA-1 is the human homologue of the murine SR-BI. CLA-1/SR-BI receptor has been suggested as a new preventative and/or therapeutic t...